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http://dx.doi.org/10.22643/JRMP.2018.4.2.45

Synthesis of oleyl-4[131I]-iodobenzoate for long-term cell trafficking  

Jeon, Hak Rim (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University)
Lee, Woonghee (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University)
Oh, Jieun (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University)
Lee, Yong Jin (Department of RI-Convergence Research, Korea Institute of Radiological and Medical Sciences)
Yoo, Jeongsoo (Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University)
Publication Information
Journal of Radiopharmaceuticals and Molecular Probes / v.4, no.2, 2018 , pp. 45-50 More about this Journal
Abstract
Great efforts are currently devoted to the development of new approaches for the labeling of cells using appropriate radionuclides. While fluoride-18 and copper-64 have been extensively studied as short-term and intermediate-term trafficking agents, iodide was studied less intensely. Here, we report a new cell labeling agent labeled with $^{131}I$, $[^{131}I]$oleyl-4-iodobenzoate ($[^{131}I]$OIB) for long-term cell trafficking. A precursor of $[^{131}I]$OIB was obtained in two steps, with the yield of 35%. The radiochemical yield of $[^{131}I]$OIB was over 50%. While $[^{131}I]$OIB could label different cells, L6 cells showed the highest cell-labeling efficiency. The $[^{131}I]$OIB-labeled L6 cells were imprinted into a rat heart, and then monitored noninvasively for 2 weeks by gamma camera imaging. We conclude that $[^{131}I]$OIB is a good candidate molecule for a long-term cell trafficking agent.
Keywords
Radiotracer; Cell trafficking agent; I-131; Gamma camera imaging;
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