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http://dx.doi.org/10.5653/cerm.2019.03496

Cichorium intybus L. extract ameliorates testicular oxidative stress induced by lead acetate in male rats  

Dorostghoal, Mehran (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz)
Seyyednejad, Seyyed Mansour (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz)
Nejad, Marzieh Noroozi Tabrizi (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz)
Publication Information
Clinical and Experimental Reproductive Medicine / v.47, no.3, 2020 , pp. 161-167 More about this Journal
Abstract
Objective: Oxidative stress has been suggested as a possible mechanism for the adverse effects of heavy metal toxicity on male reproduction. Cichorium intybus L. is used in Iranian folk medicine as a hepatoprotective agent as well as for its supposed fertility-enhancing properties. The present study was performed to investigate whether the ethanolic extract of C. intybus leaves could protect male rats against lead-induced testicular oxidative stress. Methods: In this experimental study, adult Wistar rats were treated with 0.1% lead acetate in drinking water alone or with 50, 100, or 200 mg/kg body weight of C. intybus extract via gavage once daily for 70 days. The weight of their reproductive organs, levels of serum hormones, histometric parameters of the seminiferous tubules, epidydimal sperm quality, and oxidative stress status were evaluated. Results: The testis weight, seminiferous tubule diameter, epididymal sperm count, serum testosterone level, and testicular levels of superoxide dismutase and glutathione peroxidase were significantly reduced (p< 0.05) in the lead-treated rats. Moreover, significantly (p< 0.05) higher levels of malondialdehyde were observed in the lead-exposed group compared to the control. However, the co-administration of C. intybus ethanolic extract in lead-treated rats was associated with a significant improvement in reproductive parameters. Conclusion: We conclude that C. intybus leaf extract has the potential to prevent lead-induced testicular toxicity and to suppress the adverse effects of lead on male reproductive health.
Keywords
Cichorium intybus L; Lead acetate; Oxidative stress; Reproductive health; Toxicity;
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1 Martinez-Haro M, Green AJ, Mateo R. Effects of lead exposure on oxidative stress biomarkers and plasma biochemistry in waterbirds in the field. Environ Res 2011;111:530-8.   DOI
2 Patrick L. Lead toxicity part II: the role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity. Altern Med Rev 2006;11:114-27.
3 Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health 2014;32:1-17.   DOI
4 Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl 2014;16:31-8.   DOI
5 Jurgonbski A, Milala J, Jusbkiewicz J, Zdunbczyk Z, Boguslaw Krol. Composition of chicory root, peel, seed and leaf ethanol extracts and biological properties of their non-inulin fractions. Food Technol Biotechnol 2011;49:40-7.
6 Jamshidzadeh A, Khoshnood MJ, Dehghani Z, Niknahad H. Hepatoprotective activity of Cichorium intybus L. leaves extract against carbon tetrachloride induced toxicity. Iran J Pharm Res 2006;5:41-6.
7 Gadgoli C, Mishra SH. Antihepatotoxic activity of Cichorium intybus. J Ethnopharmacol 1997;58:131-4.   DOI
8 Ahmed B, Al-Howiriny TA, Siddiqui AB. Antihepatotoxic activity of seeds of Cichorium intybus. J Ethnopharmacol 2003;87:237-40.   DOI
9 Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152-9.   DOI
10 Sakihama Y, Mano J, Sano S, Asada K, Yamasaki H. Reduction of phenoxyl radicals mediated by monodehydroascorbate reductase. Biochem Biophys Res Commun 2000;279:949-54.   DOI
11 Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 2003;91 Spec No(2):179-94.   DOI
12 Van den Ende W, Valluru R. Sucrose, sucrosyl oligosaccharides, and oxidative stress: scavenging and salvaging? J Exp Bot 2009;60:9-18.   DOI
13 Kumar S. Occupational and environmental exposure to lead and reproductive health impairment: an overview. Indian J Occup Environ Med 2018;22:128-37.
14 Jensen TK, Bonde JP, Joffe M. The influence of occupational exposure on male reproductive function. Occup Med (Lond) 2006;56:544-53.   DOI
15 Basnet P, Hansen SA, Olaussen IK, Hentemann MA, Acharya G. Changes in the semen quality among 5739 men seeking infertility treatment in Northern Norway over past 20 years (1993-2012). J Reprod Biotechnol Fertil 2016;5:1-7.
16 Meeker JD, Rossano MG, Protas B, Diamond MP, Puscheck E, Daly D, et al. Cadmium, lead, and other metals in relation to semen quality: human evidence for molybdenum as a male reproductive toxicant. Environ Health Perspect 2008;116:1473-9.   DOI
17 Telisman S, Colak B, Pizent A, Jurasovic J, Cvitkovic P. Reproductive toxicity of low-level lead exposure in men. Environ Res 2007;105:256-66.   DOI
18 Wyrobek AJ, Schrader SM, Perreault SD, Fenster L, Huszar G, Katz DF, et al. Assessment of reproductive disorders and birth defects in communities near hazardous chemical sites. III. Guidelines for field studies of male reproductive disorders. Reprod Toxicol 1997;11(2-3):243-59.   DOI
19 Eibensteiner L, Del Carpio Sanz A, Frumkin H, Gonzales C, Gonzales GF. Lead exposure and semen quality among traffic police in Arequipa, Peru. Int J Occup Environ Health 2005;11:161-6.   DOI
20 Hernandez-Ochoa I, Garcia-Vargas G, Lopez-Carrillo L, Rubio-Andrade M, Moran-Martinez J, Cebrian ME, et al. Low lead environmental exposure alters semen quality and sperm chromatin condensation in northern Mexico. Reprod Toxicol 2005;20:221-8.   DOI
21 Bolin CM, Basha R, Cox D, Zawia NH, Maloney B, Lahiri DK, et al. Exposure to lead and the developmental origin of oxidative DNA damage in the aging brain. FASEB J 2006;20:788-90.   DOI
22 Marchlewicz M, Michalska T, Wiszniewska B. Detection of leadinduced oxidative stress in the rat epididymis by chemiluminescence. Chemosphere 2004;57:1553-62.   DOI
23 de Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod 1997;2:48-54.   DOI
24 Shalan MG, Mostafa MS, Hassouna MM, El-Nabi SE, El-Refaie A. Amelioration of lead toxicity on rat liver with Vitamin C and silymarin supplements. Toxicology 2005;206:1-15.   DOI
25 Ilaiyaraja N, Khanum F. Evaluation of antioxidant and toxicological properties of chicory leaves. IJPBA 2010;1:155-63.
26 Mirhaydar H. Plants sciences: application of plants in prophylaxisand treatment of diseases. Tehran: Daftar Nashr Farhang Islami; 1993.
27 Zargari A. Medicinal plants. Tehran: Tehran University Publication; 1996.
28 Behnam Rasouli M, Hosseinzade H, Ali Akbarpour A. The effects of Soxhlet aqueous extract of Chicory leaves on the blood pH and cations level & the sex of newborns in rat. Daneshvar Med 2000;27:57-64.
29 Dorostghoal M, Seyyednejad SM, Nejad MN. Beneficial effects of Cichorium intybus L. extract on oxidative status and reproductive parameters in male Wistar rats: An experimental study. Int J Reprod Biomed (Yazd) 2019;17:425-34.
30 Das S, Vasudeva N, Sharma S. Cichorium intybus: A concise report on its ethnomedicinal, botanical, and phytopharmacological aspects. Drug Dev Ther 2016;7:1-12.   DOI
31 Kim JH, Mun YJ, Woo WH, Jeon KS, An NH, Park JS. Effects of the ethanol extract of Cichorium intybus on the immunotoxicity by ethanol in mice. Int Immunopharmacol. 2002;2:733-44.   DOI
32 Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med 2000;29:927-45.   DOI
33 Hayes W. Principle and methods of toxicology. New York: Raven Press; 1989.
34 Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, et al. Evaluation of organ weights for rodent and non-rodent toxicity studies: a review of regulatory guidelines and a survey of current practices. Toxicol Pathol 2007;35:742-50.   DOI
35 El-Sayed YS, El-Neweshy MS. Impact of lead toxicity on male rat reproduction at hormonal and histopathological levels. Toxicol Environ Chem 2010;4:765-74.   DOI
36 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.   DOI
37 Rubio J, Riqueros MI, Gasco M, Yucra S, Miranda S, Gonzales GF. Lepidium meyenii (Maca) reversed the lead acetate induced--damage on reproductive function in male rats. Food Chem Toxicol 2006;44:1114-22.   DOI
38 Sainath SB, Meena R, Supriya Ch, Reddy KP, Reddy PS. Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. Environ Toxicol Pharmacol 2011;32:146-54.   DOI
39 Anjum MR, Madhu P, Reddy KP, Reddy PS. The protective effects of zinc in lead-induced testicular and epididymal toxicity in Wistar rats. Toxicol Ind Health 2017;33:265-76.   DOI
40 Hamadouche NA, Sadi N, Kharoubi O, Slimani M, Aoues A. The protective effect of vitamin E against genotoxicity of lead acetate intraperitoneal administration in male rat. Arch Biol Sci 2013;65:1435-45.   DOI
41 Mruk DD, Cheng CY. Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocr Rev 2004;25:747-806.   DOI
42 Yoshida M, Kitani T, Takenaka A, Kudoh K, Katsuda SI, Taya K, et al. Lack of effects of oxolinic acid on spermatogenesis in young adult and aged Wistar rats. Food Chem Toxicol 2002;40:1815-25.   DOI
43 Dobrakowski M, Pawlas N, Kasperczyk A, Kozlowska A, Olewinska E, Machon-Grecka A, et al. Oxidative DNA damage and oxidative stress in lead-exposed workers. Hum Exp Toxicol 2017;36:744-54.   DOI
44 Chandra AK, Ghosh R, Chatterjee A, Sarkar M. Effects of vanadate on male rat reproductive tract histology, oxidative stress markers and androgenic enzyme activities. J Inorg Biochem 2007;101:944-56.   DOI
45 Uzunhisarcikli M, Kalender Y, Dirican K, Kalender S, Ogutcu A, Buyukkomurcu F. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pestic Biochem Physiol 2007;87:115-22.   DOI
46 Raji Y, Udoh US, Mewoyeka OO, Ononye FC, Bolarinwa AF. Implication of reproductive endocrine malfunction in male antifertility efficacy of Azadirachta indica extract in rats. Afr J Med Sci 2003;32:159-65.