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http://dx.doi.org/10.5653/cerm.2018.45.1.25

Effects of three-area laser-assisted zona thinning in 8-cell human embryos on pregnancy outcomes in vitro fertilization  

Jeong, Ju-Eun (Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University School of Medicine)
Joo, Bo-Sun (Healthy Aging Korean Medical Research Center, Pusan National University)
Kim, Chang-Woon (Department of Obstetrics and Gynecology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine)
Kim, Hwi-Gon (Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University School of Medicine)
Joo, Jong-Kil (Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University School of Medicine)
Lee, Kyu-Sup (Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University School of Medicine)
Publication Information
Clinical and Experimental Reproductive Medicine / v.45, no.1, 2018 , pp. 25-30 More about this Journal
Abstract
Objective: This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human in vitro fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos. Methods: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at $120^{\circ}$ intervals. The control group was embryos without LAZT. Of the 72 consecutive fresh cycles and the 28 FT embryo transfer cycles, the patients in 55 fresh cycles and 17 FT cycles declined LAZT, and those cycles were defined as the control group. Results: In the fresh cycles, the pregnancy rates were similar in the LAZT and control groups. However, in the FT cycles, the pregnancy rate was significantly higher in the LAZT group than in the control group (45.5% in the LAZT group vs. 23.5% in the control group, p< 0.05). Conclusion: These results show that multi-area LAZT resulted in significantly improved pregnancy outcomes in human 8-cell embryos compared to controls.
Keywords
Age; Frozen-thawed embryos; Laser- assisted hatching; Multiple thinning; Pregnancy outcome;
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