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http://dx.doi.org/10.5653/cerm.2016.43.3.146

Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders  

Hur, Yong Soo (Maria Fertility Hospital)
Ryu, Eun Kyung (Maria Fertility Hospital)
Yoon, San Hyun (Maria Fertility Research Center)
Lim, Kyung Sil (Maria Fertility Hospital)
Lee, Won Don (Maria Fertility Hospital)
Lim, Jin Ho (Maria Fertility Hospital)
Publication Information
Clinical and Experimental Reproductive Medicine / v.43, no.3, 2016 , pp. 146-151 More about this Journal
Abstract
Objective: This study was conducted to compare the effects of static culture, dynamic culture, and the combination of dynamic culture with specialized surfaces involving co-culture on human embryonic development. Embryos cultured using conventional static culture (SC) techniques served as a control group. We compared dynamic culture using micro-vibration culture (MVC) and micro-vibration with co-culture (MCoC), in which autologous cumulus cells were used as a specialized surface. Methods: We conducted a chart review of patients who were treated between January 2011 and November 2014 in order to compare embryonic development rates and pregnancy rates among the groups. Zygotes were cultured in micro-droplets, and embryos were subsequently selected for transfer. Some surplus embryos were cryopreserved, and the others were cultured for blastocyst development. A micro-vibrator was set at the frequency of 42 Hz for duration of 5 seconds per 60 minutes to facilitate embryo development. Results: No significant differences among the groups were present in patient's characteristics. However, the clinical pregnancy rates were significantly higher in the MVC group and the MCoC group than in the SC group. No significant differences were found in the blastocyst development rate between the SC group and the MVC group, but the blastocyst development rate in the MCoC group was significantly higher than in the SC and MVC groups. Conclusion: The clinical pregnancy rate was significantly increased by the application of micro-vibration to the embryonic cultures of poor responders. The blastocyst development rate was significantly increased by the application of MCoC to surplus embryos.
Keywords
Blastocyst; Coculture techniques; Embryo culture techniques;
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  • Reference
1 Swain JE. Decisions for the IVF laboratory: comparative analysis of embryo culture incubators. Reprod Biomed Online 2014;28:535-47.   DOI
2 Ali J. Continuous ultra micro-drop culture yields higher pregnancy and implantation rates than either large-drop culture or fresh-medium replacement. Clin Embryol (Online) 2004;7:1-23.
3 Gopichandran N, Leese HJ. The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos. Reproduction 2006;131:269-77.   DOI
4 Melin J, Lee A, Foygel K, Leong DE, Quake SR, Yao MW. In vitro embryo culture in defined, sub-microliter volumes. Dev Dyn 2009;238:950-5.   DOI
5 Rieger D, Schimmel T, Cohen J, Cecchi M. Comparison of GPS and standard dishes for embryo culture: set-up and observation times, and embryo development. Proceedings of the 14th World Congress on In Vitro Fertilization; 2007 Sep 15-19; Montreal, Canada.
6 Vajta G, Peura TT, Holm P, Paldi A, Greve T, Trounson AO, et al. New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system. Mol Reprod Dev 2000;55:256-64.   DOI
7 Vajta G, Korosi T, Du Y, Nakata K, Ieda S, Kuwayama M, et al. The Well-of-the-Well system: an efficient approach to improve embryo development. Reprod Biomed Online 2008;17:73-81.   DOI
8 Anand S, Guha SK. Mechanics of transport of the ovum in the oviduct. Med Biol Eng Comput 1978;16:256-61.   DOI
9 Fauci LJ, Dillon R. Biofluidmechanics of reproduction. Annu Rev Fluid Mech 2006;38:371-94.   DOI
10 Lyons RA, Saridogan E, Djahanbakhch O. The reproductive significance of human Fallopian tube cilia. Hum Reprod Update 2006;12:363-72.   DOI
11 Zervomanolakis I, Ott HW, Hadziomerovic D, Mattle V, Seeber BE, Virgolini I, et al. Physiology of upward transport in the human female genital tract. Ann N Y Acad Sci 2007;1101:1-20.   DOI
12 Raty S, Walters EM, Davis J, Zeringue H, Beebe DJ, Rodriguez-Zas SL, et al. Embryonic development in the mouse is enhanced via microchannel culture. Lab Chip 2004;4:186-90.   DOI
13 Cabrera LM, Heo YS, Ding J, Takayama S, Smith GD. Improved blastocyst development with microfluidics and Braille pin actuator enabled dynamic culture. Fertil Steril 2006;86:S43.
14 Mizobe Y, Yoshida M, Miyoshi K. Enhancement of cytoplasmic maturation of in vitro-matured pig oocytes by mechanical vibration. J Reprod Dev 2010;56:285-90.   DOI
15 Isachenko E, Maettner R, Isachenko V, Roth S, Kreienberg R, Sterzik K. Mechanical agitation during the in vitro culture of human pre-implantation embryos drastically increases the pregnancy rate. Clin Lab 2010;56:569-76.
16 Hunter SK, Scott JR, Hull D, Urry RL. The gamete and embryo compatibility of various synthetic polymers. Fertil Steril 1988;50:110-6.   DOI
17 Hunter RH. Modulation of gamete and embryonic microenvironments by oviduct glycoproteins. Mol Reprod Dev 1994;39:176-81.   DOI
18 Franks F. Solute-water interactions: do polyhydroxy compounds after the properties of water? Cryobiology 1983;20:335-45.   DOI
19 Gardner DK, Rodriegez-Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer. Hum Reprod 1999;14:2575-80.   DOI
20 Thompson JG. Culture without the petri-dish. Theriogenology 2007;67:16-20.   DOI
21 Villa-Diaz LG, Nandivada H, Ding J, Nogueira-de-Souza NC, Krebsbach PH, O’Shea KS, et al. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat Biotechnol 2010;28:581-3.   DOI
22 Hur YS, Park JH, Ryu EK, Park SJ, Lee JH, Lee SH, et al. Effect of micro-vibration culture system on embryo development. J Assist Reprod Genet 2013;30:835-41.   DOI
23 Lee SW, Yoon SH, Yoon HG, Cho HJ, Heo YS, Yoon HJ, et al. The studies on the development of human blastocyst embryos in IVF-ET Program. II: the development of human blastocyst embryos by co-culture with cumulus cells. Korean J Fertil Steril 1998;25:35-42.
24 Goto K, Kajihara Y, Kosaka S, Koba M, Nakanishi Y, Ogawa K. Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes. J Reprod Fertil 1988;83:753-8.   DOI
25 Matsuura K, Hayashi N, Kuroda Y, Takiue C, Hirata R, Takenami M, et al. Improved development of mouse and human embryos using a tilting embryo culture system. Reprod Biomed Online 2010;20:358-64.   DOI
26 Goovaerts IG, Leroy JL, Van Soom A, De Clercq JB, Andries S, Bols PE. Effect of cumulus cell coculture and oxygen tension on the in vitro developmental competence of bovine zygotes cultured singly. Theriogenology 2009;71:729-38.   DOI
27 Benkhalifa M, Demirol A, Sari T, Balashova E, Tsouroupaki M, Giakoumakis Y, et al. Autologous embryo-cumulus cells co-culture and blastocyst transfer in repeated implantation failures: a collaborative prospective randomized study. Zygote 2012;20:173-80.   DOI
28 Swain JE, Smith GD. Advances in embryo culture platforms: novel approaches to improve preimplantation embryo development through modifications of the microenvironment. Hum Reprod Update 2011;17:541-57.   DOI
29 Jenkins JM, Davies DW, Devonport H, Anthony FW, Gadd SC, Watson RH, et al. Comparison of ‘poor’ responders with ‘good’ responders using a standard buserelin/human menopausal gonadotrophin regime for in-vitro fertilization. Hum Reprod 1991;6:918-21.   DOI
30 Keay SD, Liversedge NH, Mathur RS, Jenkins JM. Assisted conception following poor ovarian response to gonadotrophin stimulation. Br J Obstet Gynaecol 1997;104:521-7.   DOI
31 Ferraretti AP, La Marca A, Fauser BC, Tarlatzis B, Nargund G, Gianaroli L, et al. ESHRE consensus on the definition of ‘poor response’ to ovarian stimulation for in vitro fertilization: the Bologna criteria. Hum Reprod 2011;26:1616-24.   DOI
32 Kang SM, Lee SW, Jeong HJ, Yoon SH, Koh MW, Lim JH, et al. Clinical outcomes of elective single morula embryo transfer versus elective single blastocyst embryo transfer in IVF-ET. J Assist Reprod Genet 2012;29:423-8.   DOI
33 Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod 1997;12:1545-9.   DOI
34 Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril 2000;73:1155-8.   DOI
35 Hickman DL, Beebe DJ, Rodriguez-Zas SL, Wheeler MB. Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos. Comp Med 2002;52:122-6.
36 Isachenko V, Maettner R, Sterzik K, Strehler E, Kreinberg R, Hancke K, et al. In-vitro culture of human embryos with mechanical micro-vibration increases implantation rates. Reprod Biomed Online 2011;22:536-44.   DOI
37 Blake JR, Vann PG, Winet H. A model of ovum transport. J Theor Biol 1983;102:145-66.   DOI
38 Heo YS, Cabrera LM, Bormann CL, Shah CT, Takayama S, Smith GD. Dynamic microfunnel culture enhances mouse embryo development and pregnancy rates. Hum Reprod 2010;25:613-22.   DOI