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http://dx.doi.org/10.5653/cerm.2013.40.2.67

Peroxisome proliferator-activated receptor ${\gamma}$ agonist suppresses human telomerase reverse transcriptase expression and aromatase activity in eutopic endometrial stromal cells from endometriosis  

Chang, Hye Jin (Health Promotion Center, Seoul National University Bundang Hospital, Seoul National University College of Medicine)
Lee, Jae Hoon (Department of Obstetrics and Gynecology, Ajou University School of Medicine)
Hwang, Kyung Joo (Department of Obstetrics and Gynecology, Ajou University School of Medicine)
Kim, Mi Ran (Department of Obstetrics and Gynecology, Ajou University School of Medicine)
Yoo, Jung Hyun (Department of Obstetrics and Gynecology, Bundang Jesaeng Hospital)
Publication Information
Clinical and Experimental Reproductive Medicine / v.40, no.2, 2013 , pp. 67-75 More about this Journal
Abstract
Objective: To investigate the effect of peroxisome proliferator activated receptor ${\gamma}$(PPAR${\gamma}$) agonist on the cell proliferation properties and expression of human telomerase reverse transcriptase (hTERT) and aromatase in cultured endometrial stromal cell (ESC) from patients with endometriosis. Methods: Human endometrial tissues were obtained from women with endometriosis and healthy women (controls) using endometrial biopsy. Isolated ESCs were cultured and the cell proliferation was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and expression of hTERT, aromatase, and cyclooxygenase (COX)-2 by western blotting according to the addition of rosiglitazone (PPAR${\gamma}$ agonist). Results: We demonstrate that the cultured ESCs of endometriosis showed hTERT protein overexpression and increased cellular proliferation, which was inhibited by rosiglitazone, in a dose-dependent manner. At the same time, PPAR${\gamma}$ agonist also inhibited aromatase and COX-2 expression, resulting in decreased prostaglandin $E_2$ production in the ESCs of endometriosis. Conclusion: This study suggests that PPAR${\gamma}$ agonist plays an inhibitory role in the proliferative properties of eutopic endometrium with endometriosis by down-regulation of hTERT and COX-2 expression; this could be a new treatment target for endometriosis.
Keywords
Endometriosis; Peroxisome proliferator activated receptor ${\gamma}$; Rosiglitazone; Human telomerase reverse transcriptase; Aromatase; Endometrium;
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