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http://dx.doi.org/10.5653/cerm.2012.39.2.73

Effects of hypoxia inducible factors-$1{\alpha}$ on autophagy and invasion of trophoblasts  

Choi, Jong-Ho (Department of Biomedical Science, CHA University)
Lee, Hyun-Jung (CHA Placenta Institute, CHA University)
Yang, Tae-Hyun (Department of Biomedical Science, CHA University)
Kim, Gi Jin (Department of Biomedical Science, CHA University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.39, no.2, 2012 , pp. 73-80 More about this Journal
Abstract
Objective: This study was undertaken to determine the effect of hypoxia inducible factor (HIF)-$1{\alpha}$ on the cell death, autophagy, and invasion of trophoblasts. Methods: To understand the effect of HIF-$1{\alpha}$, we inhibited HIF-$1{\alpha}$ using siRNA under normoxia and hypoxia conditions. Invasion assay and zymography were performed to determine changes in the invasion ability of HIF-$1{\alpha}$. Western blotting and immunofluorescence were performed to determine some of the signal events involved in apoptosis and autophagy. Results: There was no difference in cell death through the inhibition of HIF-$1{\alpha}$ expression by siRNA; however, the expression of LC3 and autophagosome formation increased. On the other hand, autophagy was increased, and the invasive ability of trophoblast cells decreased according to the inhibition of HIF-$1{\alpha}$ expression by siRNA. These experimental results mean that HIF-$1{\alpha}$ genes regulate the invasive ability of trophoblasts by increasing autophagy. Conclusion: This study contributes important data for understanding the mechanism of early pregnancy implantation and the invasive ability of trophoblasts by defining the relationship between the roles of HIF-$1{\alpha}$ and autophagy.
Keywords
Placenta; Trophoblasts; Hypoxia; Invasiveness; Programmed cell death type II;
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