Browse > Article

Comparative Results of Embryo Development and Clinical Pregnancy using Sperm Retrieved from Fresh and Frozen-thawed Testicular Tissue from Patients with Obstructive and Non-obstructive Azoospermia  

Park, Yong-Seog (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Choi, Su-Jin (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Lee, Sun-Hee (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Park, Dong-Wook (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Lim, Chun-Kyu (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Jun, Jin-Hyun (Laboratory of Reproductive Biology and Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Koong, Mi-Kyoung (Department of Obstetrics and Gynecology, Kwandong University)
Park, Chan-Woo (Department of Obstetrics and Gynecology, Kwandong University)
Song, In-Ok (Department of Obstetrics and Gynecology, Kwandong University)
Seo, Ju-Tae (Department of Urology, Kwandong University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.36, no.4, 2009 , pp. 301-310 More about this Journal
Abstract
Objective: To compare the embryonic development and pregnancy results using sperms retrieved from fresh and frozen-thawed testicular tissue in patients with obstructive (OA) and non-obstructive azoospermia (NOA). Methods: A total two hundred twenty-two cycles of TESE-ICSI were performed in OA and NOA. Sperms were retrieved from fresh and frozen-thawed testicular tissue. ICSI was performed patient's own sperm. Fertilization was assessed 16~18 hrs after ICSI. Embryo development and pregnancy rates were analysed. Results: The fertilization rates were significantly different between OA and NOA patients (75.2% vs. 56.7%, p<0.05), however, embryo development did not differ between the groups (96.9% vs. 98.0%). Likewise, OA and NOA groups had no differences in their clinical pregnancy and delivery rates, 33.9% vs. 36.0% and 28.1% vs. 28.0%, respectively. With regard to sperm retrieved from fresh testicular tissue, fertilization rates were significantly different between the OA and NOA groups (76.4% vs. 52.9%, p<0.05); however, embryo development, clinical pregnancy and delivery rates were not different. For sperm retrieved from thawed testicular tissue, the fertilization rates were significantly different between the two groups (74.7% OA group vs. 65.6% NOA group, p<0.05); however, embryo development, clinical pregnancy and delivery rates were not different. Conclusions: Embryo development and clinical pregnancy did not differ in patients with obstructive and non-obstructive azoospermia, whether sperm retrieved from fresh and thawed testicular tissue were used, although the fertilization rates were different. Therefore, ICSI with sperm retrieved from fresh and thawed testicular tissue could achieve relevant clinical pregnancy results in patients with azoospermia.
Keywords
Obstructive azoospermia; Non-obstructive azoospermia; Cryopreservation; Testicular sperm; TESE-ICSI; Embryo development;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Devroey P, Liu J, Nagy PZ, Goossens A, Tournaye H, Camus M, et al. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod 1995; 10: 1457-60   PUBMED   ScienceOn
2 Romero J, Remohi J, Minguez Y, Rubio C, Pellicer A, Gil-Salom M. Fertilization after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Fertil Steril 1996; 65: 877-9   PUBMED   ScienceOn
3 Oates RD, Mulhall J, Burgess C, Cunningham D, Carson R Fertilization and pregnancy using intentionally cryopreserved testicular tissue as the sperm source for intracytoplasmic sperm injection in 10 men with nonobstructive azoospermia. Hum Reprod 1997; 12: 734-9   DOI   ScienceOn
4 Park YS, Lee SH, Song SJ, Jun JH, Koong MK, Seo JT. Influence of motility on the outcome of in vitro fertilization/ intracytoplasmic sperm injection with fresh vs. frozen testicular sperm from men with obstructive azoospermia. Fertil Steril 2003; 80: 526-30   DOI   ScienceOn
5 Tesarik J, Greco E. The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology. Hum Reprod 1999; 14: 1318-23   DOI   ScienceOn
6 Friedler S, Raziel A, Soffer Y, Strassburger D, Komarovsky D, Ron-El R. Intracytoplasmic injection of fresh and cryopreserved testicular spermatozoa in patients with nonobstructive azoospermia-a comparative study. Fertil Steril 1997; 68: 892-7   DOI   ScienceOn
7 Kupker W, Schlegel PN, Al-Hasani S, Fornara P, Johannisson R, Sandmann J, et al. Use of frozen-thawed testicular sperm for intracytoplasmic sperm injection. Fertil Steril 2000; 73: 453-8   DOI   ScienceOn
8 Mercan R, Urman B, Alatas C, Aksoy S, Nuhoglu A, Isiklar A et al. Outcome of testicular sperm retrieval procedures in non-obstructive azoospermia: percutaneous aspiration versus open biopsy. Hum Reprod 2000; 15: 1548-51   DOI   ScienceOn
9 Kahraman S, Ozgur S, Alatas C, Aksoy S, Tasdemir M, Nuhoglu A, et al. Fertility with testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermic men. Hum Reprod 1996; 11: 756-60   DOI   PUBMED
10 Ben-Yosef D, Yogev L, Hauser R, Yavetz H, Azem F, Yovel I, et al. Testicular sperm retrieval and cryopreservation prior to initiating ovarian stimulation as the first line approach in patients with non-obstructive azoospermia. Hum Reprod 1999; 14: 1794-801   DOI   PUBMED
11 Prabakaran SA, Agarwal A, Sundaran A, Thomas AJ, Sikka S. Cryosurvival of testicular spermatozoa from obstructive patients: The Cleveland Clinical Experience. Fertil Steril 2006; 86: 1789-91   DOI   ScienceOn
12 Kahraman S, Kumtepe Y, Sertyel S, Donmez E, Benkhalifa M, Findikli N, et al. Pronuclear morphology scoring and chromosomal status of embryos in severe male infertility. Hum Reprod 2002; 17: 3193-200   DOI   ScienceOn
13 Wood S, Thomas K, Schnauffer K, Troup S, Kingsland C, Lewis-Jones I. Reproductive potential of fresh and cryopreserved epididymal and testicular spermatozoa in consecutive intracytoplasmic sperm injection cycles in the same patients. Fertil Steril 2002; 77: 1162-6   DOI   ScienceOn
14 Marques de Oliveira N, Sanchez RV, Fiesta SR, Salgado TL, Rodriguez R, Bethencourt JCA, et al. Pregnancy with frozenthawed and fresh testicular biopsy after motile and immotile sperm microinjection, using the mechanical touch technique to assess viability. Hum Reprod 2004; 19: 262-5   DOI   ScienceOn
15 Shoukir Y, Chardonnens D, Campana A, Sakkas D. Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence? Hum Reprod 1998; 13: 1632-7   DOI   ScienceOn
16 Schill T, Bals-Pratsch M, Kupker W, Sandmann J, Johannisson R, Diedrich K. Clinical and endocrine follow-up of patients after testicular sperm extraction. Fertil Steril 2003; 79: 281-6   DOI   ScienceOn
17 Rosenlund B, Kvist U, Ploen L, Lundh Rozell B, Sjoblom P, Hillensjo T. A comparison between open and percutaneous needle biopsies in men with azoospermia. Hum Reprod 1998;13: 1266-71   DOI   ScienceOn
18 Kamal A, Fahmy I, Mansour RT, Abou-Setta AM, Serour GI, Aboulghar MA. Selection of individual testicular tubules from biopsied testicular tissue with a stereomicroscope improves sperm retrieval rate. J Androl 2004; 25: 123-7   PUBMED   ScienceOn
19 Vernaeve V, Tournaye H, Osmanagaoglu K, Verheyen G, Van Steirteghem A, Devroey P. Intracytoplasmic sperm injection with testicular spermatozoa is less successful in men with nonobstructive azoospermia than in men with obstructive azoospermia. Fertil Steril 2003; 79: 529-33   DOI   ScienceOn
20 Tournaye H, Verheyen G, Nagy P, Ubaldi F, Goossens A, Silber S, et al. Are there any predictive factors for successful testicular sperm recovery in azoospermic patients? Hum Reprod 1997; 12: 80-6   DOI   PUBMED   ScienceOn
21 Nicopoullos JDM, Gilling-Smith C, Almeida PA, Ramsay JWA. The results of 154 ICSI cycles using surgically retrieved sperm from azoospermic men. Hum Reprod 2004; 19: 579-85   DOI   ScienceOn
22 Kone J, Kanyo K, Cseh S. Deliveries from embryos fertilized with spermatozoa obtained from cryopreserved testicular tissue. J Assisted Reprod Genet 2006; 23: 247-52   DOI   ScienceOn
23 Plas E, Riedl CR, Engelhardt PF, Muhlbauer H, Pfluger H. Unilateral or bilateral testicular biopsy in the era of intracytoplasmic sperm injection. J Urol 1999; 162: 2010-3   DOI   ScienceOn
24 Park YS, Lee HS, Byun HK, Youm HW, Song SJ, Lim CK, et al. Influence of motility of fresh and frozen testicular spermatozoa on the pregnancy rate in obstructive azoospermic patients. Kor J Fertil Steril 2001; 28: 155-60   과학기술학회마을   ScienceOn
25 Dafopoulos K, Griesinger G, Schultz-Mosgau A, Orief Y, Schopper B, Nikolettos N, et al. Factors affecting outcome after ICSI with spermatozoa retrieved from cryopreserved testicular tissue in non-obstructive azoospermia. Reprod Bio- Med 2005; 10: 455-60   DOI   ScienceOn
26 Schlegel PN, Palermo GD, Goldstein M, Menendez S, Zaninovic N, Veeck LL. Testicular sperm extraction with intracytoplasmic sperm injection for nonobstructive azoospermia. Urol 1997; 49: 435-40   DOI   ScienceOn
27 Gianaroli L, Magli MC, Selman HA, Colpi G, Belgrano E, Trombetta C, et al. Diagnostic testicular biopsy and cryopreservation of testicular tissue as an alternative to repeated surgical openings in the treatment of azoospermic men. Hum Reprod 1999; 14: 1034-8   DOI   ScienceOn
28 Tesarik J, Junca AM, Hazout A, Aubriot FX, Nathan C, Cohen-Bacrie P, et al. Embryos with high implantation potential after intracytoplasmic sperm injection can be recognized by a simple, non-invasive examination of pronuclear morphology. Hum Reprod 2000; 15: 1396-9   DOI   ScienceOn
29 Song GJ, Lee HS, Park YS, Lee HJ, Lee YS, Seo JT, et al. Expression pattern of germ cell-specific genes in the testis of patients with nonobstructive azoospermia: usefulness as a molecular marker to predict the presence of testicular sperm. Fertil Steril 2000; 73: 1104-8   DOI   ScienceOn
30 Rhouma KB, Marrakchi H, Khouja H, Attalah K, Miled EB, Sakly M. Outcome of intracytoplasmic injection of fresh and frozen-thawed testicular spermatozoa. J Reprod Med 2003; 48: 349-54   PUBMED   ScienceOn
31 Habermann H, Seo R, Cieslak J, Niederberger C, Prins GS, Ross L. In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa. Fertil Steril 2000; 73: 955-60   DOI   ScienceOn
32 Nogueira D, Bourgain C, Verheyen G, Van Steirteghem AC. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies. Hum Reprod 1999; 14: 2041-9   DOI   PUBMED
33 Palermo GD, Schlegel PN, Hariprashad JJ, Ergun B, Mielnik A, Zaninovic N. Fertilization and pregnancy outcome with intracytoplasmic sperm injection for azoospermic men. Hum Reprod 1999; 14: 741-8   DOI   ScienceOn
34 De Croo I, Van der Elst J, Everaert K, De Sutter P, Dhont M. Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia. Hum Reprod 2000; 15: 1383-8   DOI   ScienceOn
35 Pasqualotto FF, Rossi-Ferragut LM, Rocha CC, Iaconelli A, Borges E. Outcome of in vitro fertilization and intracytoplasmic injection of epididymal and testicular sperm obtained from patients with obstructive and nonobstructive azoospermia. J Urol 2002; 167: 1753-6   DOI   ScienceOn
36 De Croo I, Van der Elst J, Everaert K, De Sutter P, Dhont M. Fertilization, pregnancy and embryo implantation rates after ICSI with fresh or frozen-thawed testicular spermatozoa. Hum Reprod 1998; 13: 1893-7   DOI   ScienceOn
37 Stalf T, Mehnert C, Hajimohammad A, Manolopoulos K, Shen Y, Schuppe H-C, et al. Influence of motility and vitality in intracytoplasmic sperm injection with ejaculated and testicular sperm. Andrologia 2005; 37: 125-30   DOI   ScienceOn
38 Park YS, Lee SH, Han SC, Choi SJ, Jun JH, Koong MK, et al. Fertilization, embryonic development, and pregnancy rate using fresh and frozen testicular sperm in hypospermatogenesis. Kor J Androl 2006; 24: 89-93
39 Balaban B, Urman B, Isiklar A, Alatas C, Mercan R, Aksoy S, et al. Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa. Hum Reprod 2001; 16: 125-9   DOI   ScienceOn
40 Gil-Salom M, Romero J, Minguez Y, Molero MD, Remohi J, Pellicer A. Testicular sperm extraction and intracytoplasmic sperm injection: a chance of fertility in nonobstructive azoospermia. J Urol 1998; 160: 2063-7   DOI   ScienceOn
41 Allan JA, Cotman AS. A new method for freezing testicular biopsy sperm: three pregnancies with sperm extracted from cryopreserved sections of seminiferous tubule. Fertil Steril 1997; 68: 741-4   DOI   ScienceOn
42 Harrington TG, Schauer D, Gilbert BR. Percutaneous testis biopsy: an alternative to open testicular biopsy in the evaluation of the subfertile man. J Androl 1996; 156: 1647-51
43 Verheyen G, Nagy Z, Joris H, Croo ID, Tournaye H, Van Steirteghem AC. Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro matured germinal vesicle stage oocytes. Fertil Steril 1997; 67: 74-80   DOI   ScienceOn