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Expression of Decidual Natural Killer (NK) Cells in Recurrent Abortion Patients with Increased Peripheral NK Cells  

Lee, Hyun-Joo (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
Kim, Sun-Haeng (Department of Obstetrics and Gynecology School of Medicine, Korea University)
Yang, Kwang-Moon (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
Park, Dong-Wook (Laboratory of Reproductive Biology & Infertility, Korea University)
Park, Chan-Woo (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
Hong, Sung-Ran (Department of Pathology, Korea University)
Cho, Dong-Hee (Department of Clinical Pathology, Korea University)
Song, In-Ok (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
Koong, Mi-Kyoung (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
Kang, Inn-Soo (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
Publication Information
Clinical and Experimental Reproductive Medicine / v.36, no.3, 2009 , pp. 199-207 More about this Journal
Abstract
Objectives: The purpose of this study was to compare the decidual NK cell populations between increased pre-conceptional peripheral NK cell population and normal pre-conceptional peripheral NK cell population in women with a history of recurrent abortion. Methods: Fourteen women with history of recurrent abortion and elevated pre-conceptional peripheral NK cell, above 15% of peripheral lymphocyte population were included in this study. As a control, twelve women with history of recurrent abortion and their peripheral NK cell percentage showed below 15% were included. Distribution of $CD56^+$ and $CD16^+$ NK cells in paraffin embedded decidual tissues including implantation sites were examined by immunohistochemical staining using anti-CD56, 16 monoclonal antibodies. After immuohistochemical staining, the numbers of decidual NK cells were counted and compared these results between study and control groups. Results: There was significant difference in decidual $CD56^+$ NK cell count ($170.1{\pm}132.1$ vs. $68.3{\pm}66.1$, p=0.02) between increased peripheral $CD56^+$ NK cell group and control group. But, there showed no statistically significant correlation between decidual $CD56^+$ NK cell count and peripheral $CD56^+$ NK cell percentage (r=0.229, p=0.261). Also there was no statistically difference decidual $CD16^+$ NK cell count between study and control group ($25.70{\pm}11.72$ vs. $31.17{\pm}22.67$), and no correlation between decidual $CD16^+$ NK cell and peripheral $CD16^+$ NK cell percentage (r=-1.40, p=0.535). Conclusions: This study shows that decidual $CD56^+$ NK cell are significantly increased in decidua of women exhibiting a history of recurrent abortion with increased $CD56^+$ peripheral NK cell. This study suggests that the percentage of peripheral NK cell reflect the expression of decidual NK cell. Consequently, pre-conceptional peripheral blood NK cell population can be the useful marker for detecting the risk of subsequent miscarriage.
Keywords
Peripheral $CD56^+$ NK cell; Decidual $CD56^+$ NK cell; Recurrent abortion;
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