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Seminal Tumor Necrosis Factor-alpha Level and Sperm Nuclear DNA Integrity in Healthy Donors  

Kim, Hyun-Jun (Department of Obstetrics and Gynecology, School of Medicine, Konkuk University)
Jee, Byung-Chul (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
Moon, Jeong-Hee (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
Lee, Jung-Ryeol (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
Suh, Chang-Suk (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
Kim, Seok-Hyun (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.36, no.1, 2009 , pp. 35-43 More about this Journal
Abstract
Objectives: Seminal concentration of tumor necrosis factor-alpha (TNF-${\alpha}$) relevant to sperm nuclear DNA integrity has not been studied. The present study aimed to evaluate seminal concentration of TNF-${\alpha}$ in correlation with sperm parameters and nuclear DNA integrity in asymptomatic healthy donors. Methods: Semen samples were obtained by masturbation from forty-five healthy donors. Results: Sperm quality was assessed by computer-assisted semen analysis and nuclear DNA integrity measured by the TUNEL assay in raw semen. TNF-${\alpha}$ concentrations were measured by ELISA in frozen-thawed seminal plasmas. Sperm DNA fragmentation rates were ranged between 1.9% and 53.0% (mean${\pm}$SD, 12.4${\pm}$9.6%). Univariate analysis revealed that DNA fragmentation rate was not associated with sperm concentration or motility but had a correlation with linearity negatively (r=-0.325, p=0.03) and age positively (r=0.484, p=0.001). The mean seminal concentration of TNF-${\alpha}$ was 4.9 pg/mL with a range from 1.1 to 22.6 pg/mL. The TNF-${\alpha}$ concentration had no correlation with clinically relevant parameters of sperm quality or nuclear DNA fragmentation rate. Conclusion: Our results indicate that sperm nuclear DNA fragmentation may be not associated with seminal TNF-${\alpha}$ level or sperm quality in asymptomatic healthy donors.
Keywords
Sperm; Nuclear integrity; DNA fragmentation; TUNEL; Tumor necrosis factor-alpha;
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