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Efficacy of Assisted Hatching by Laser in Human IVF-ET Program  

Lee, Jung-Hyun (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Han, Ji-Eun (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Kim, You-Shin (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Won, Hyung-Jae (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Cho, Chung-Hyun (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Kwak, In-Pyung (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Eum, Jin-Hee (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Park, Eun-A (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Choi, Yoon-Jung (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Lee, Dong-Ryul (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Yoon, Tae-Ki (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.35, no.3, 2008 , pp. 193-202 More about this Journal
Abstract
Objective: To evaluate efficacy of assisted hatching by laser (AHL) and acidified Tyrode solution (AHA) in selected groups of IVF-ET patients who have a poor prognosis. Methods: From February 2006 to September 2006, total of 328 IVF-ET cycles with advanced female age (${\geq}38$ years), recurrent implantation failure (${\geq}3$ cycles), thick zona (${\geq}17{\mu}m$), and/or poor quality of embryo were randomly divided into assisted hatching by acidified Tyrode solution (AHA, n=180) and the assisted hatching using the ZILOS-tk laser (AHL, n=148) groups. Clinical outcomes were analyzed and compared between AHA and AHL group based on the patient characteristics. Results: In all AHL and AHA group, there were no significant differences in pregnancy (42.6%, 63/148 vs. 33.3%, 60/180) and implantation rates (17.4%, 82/470 vs. 16.0%, 89/556) However, in advanced female age group (Group 1), pregnancy (37.0%, 20/54 vs. 18.7%, 14/75) and implantation rates (14.4%, 23/160 vs. 7.1%, 15/210) in AHL group were significantly (p<0.05) higher than those of AHA, although there was no difference in patient parameters of both groups. And, the clinical outcome of groups with recurrent implantation failure (Group 2), thick zona pellucida (Group 3) and poor quality embryo (Group 4) were improved in AHL compared to those of AHA: 43.8% (21/48) and 31.6% (25/79) in Group 2, 43.8% (32/73) and 34.1% (28/82) in Group 3, 25.0% (7/28) and 14.6% (6/41) in Group 4, but no significance. Conclusion: The AHL improved the pregnancy and implantation rates in patients with advanced female age and recurrent implantation failure when compared to outcomes achieved from AHA. Therefore, this AHL technique may be a efficient and safe method for patients with poor prognosis.
Keywords
Assisted hatching; Laser; Acidified Tyroid's solution; IVF-ET; Clinical outcome;
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1 Edwards RG, Fishel SB, Cohen J. Factors influencing the success of in-vitro fertilization for alleviating human infertility. J In Vitro Fert Embryo Trans 1986; 1: 3-23   과학기술학회마을
2 Blake DA, Forsberg AS, Johansson BR, Wikland M. Laser zona pellucida thinning- an alternative approach to assisted hatching. Hum Reprod 2001; 16: 1959-64   DOI   ScienceOn
3 Cohen J. Assisted hatching of human embryo J In Vitro Fertil Embryo Transfer 1991; 8: 179-90   DOI
4 Stein A Rufas O, Amit S, Averech O, Pinkas H, Ovadia J. Assisted hatching by partial zona dissection of human preembryo in patients with recurrent implantation failure after in vitro fertilization. Fertil Steril 1995; 63: 838-41   DOI   PUBMED
5 Makrakis E, Angeli I, Agapitou K, Pappas K, Dafereras A, Panos K. Laser versus mechanical assisted hatching; a prospective study of clinical outcomes Fertil Steril 2006; 86: 1596-9   DOI   ScienceOn
6 Al-Nuaim LA, Jenkins JM. Assisted hatching in assisted reproduction. An International Journal of Obst Gynaecol 2002; 109: 856-62   DOI
7 Stromer H, Feichting W. Sucessful clinical application of laser for micromanipulation in an in vitro fertilization program. Fertil Steril 1992; 58: 212-4   DOI   PUBMED
8 Veeck LL. Preembryo grading. In: Veeck LL, editor. Atlas of human oocyte and early conceptus. Baltimore: Williams & Wikins; 1991. p.121-49
9 Balanban B, Urman B, Alatas C, Mercan R, Mumcu A, Isiklar A. A comparison of four different techniques of assisted hatching. Hum Reprod 2002; 17: 1239-43   DOI   ScienceOn
10 Lanzendorf SE, Ratts VS, Moley KH, Goldstein JS, Dahan MH, Odem RR. A randomized, prospective study comparing laser-assisted hatching and assisted hatching using acidified medium. Fertil Steril 2007; 87: 1450-6   DOI   ScienceOn
11 Montag M, Vander Ven H. Laser-assisted hatching in assisted reproduction. Croat Med J 1999; 40: 398-403   PUBMED
12 Yaron Y, Botchan A, Amit A. Endometrial receptivity in the light of modern assisted reproductive technologies. Fertil Steril 1994; 62: 225-32   DOI   PUBMED
13 Liu HC, Rosenwaks Z. Early pregnancy wastage in in vitro fertilization patients. J. In Vitro Fertil Embryo Transf 1990; 8: 65-72
14 Van Kooij RJ, Looman CW, Habbema JD, Dorland M, Te Velde ER. Age-dependent decrease in embryo implantation rate after in vitro fertilization. Fertil steril 1996; 66: 769-75   DOI   PUBMED
15 Liu hc, Cohen J, Alikani M, Noyes N, Rosenwaks Z. Assisted hatching facilitates earlier implantation. Fertil Steril 1993; 60: 871-5   DOI   PUBMED
16 Vosa D, Van steirteghem A. Zona harding, zona drilling and assisted hatching; new achievements in assisted reproduction. Cell Tissues Organs 2000; 166: 220-7   DOI   ScienceOn
17 Kanyo K, Konc J. A follow-up study of children born after diode laser assisted hatching. Eur J obstet Gynecol reprod biol 2003; 110: 117-80   DOI   ScienceOn
18 Douglas-Hamilton DH, Conia J, Thermal effects in laserassisted preembryo zona drilling. J. Biomed Optics 2001; 6: 205-13   DOI   ScienceOn
19 Rink k, Delarcretaz G, Salathe RP, Senn A, Nocera D, Germond M, et al. Non-contact microdrilling of mouse zona pellucida with an objective-delivered 1.48-microns diode laser. Laser Surg Med 1996; 18: 52-62   DOI   ScienceOn
20 Schiewe MC, Hazeleger NL, Sclimenti C, Balmaceda JP. Physiological characterization of blastocyst hatching mechanism by use of mouse anti-hatching mode. Fertil Steril 1995; 63: 288-94   DOI   PUBMED
21 Malter HE, Cohen J. Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryo. Gamete Res 1989; 24: 67-80   DOI
22 Khalifa EAM, Tucker MJ, Hunt P. Cruciate thinning of zona pellucida for more successful enhancement of blastocyst hatching in the mouse. Hum Reprod 1992; 7: 532-6   DOI   PUBMED
23 Hsieh YY, Huang CC, Cheng T, Chang CC, Tsai HD, Lee MS. Laser-assisted hatching of embryo is better than chemical method for enhancing the pregnancy rate in women with advanced age. Fertil Steril 2002; 78: 179-82   DOI   ScienceOn
24 Germond M, Nocera D, Senn A, Rink K, Delacretaz G, Fakan S. Microdissection of mouse and human zona pellucida using 1.48 ${\mu}m$ diode laser beam; efficacy and safety of the procedure Fertil Steril 1995; 64: 604-11   DOI
25 Cohen J, Elsner C, Kort H. Impairment of hatching process following IVF in the human and improvement of implantation by assisted hatching using micromanipulation. Hum Reprod 1990; 5: 7-13   PUBMED
26 Cohen J, Alikani M, Trowbridge J. Implantation enhancement by selected assisted hatching using zona drilling of human embryo with poor prognosis. Hum Reprod 1992; 7: 685-91   DOI   PUBMED
27 Gordon JW, Dapunt U. A new mouse model for embryo with a hatching deficiency and its use to elucidate the mechanism of blastocyst hatching. Fertil Steril 1993; 59: 1296-301   DOI   PUBMED
28 Wong BC, Boyd CA, Lanzendorf SE. Randomized controlled study of human zona pellucida dissection using the Zona Infrared Laser Optical System: evaluation of blastomere damage, embryo development, and subsequent hatching. 2003; 80: 1249-54   DOI   ScienceOn
29 Germond M, Nocera D, Senn A, Rink K, Delacretaz G, Pedrazzini T. Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 microm diode laser beam. Hum Reprod 1996; 11: 1043-8   DOI   ScienceOn
30 Magli MC, Gianaroli L, Ferraretti AP, Fortini D, Aicardi G, Montanaro N. Rescue of implanatation potential in embryo with poor prognosis by assisted zona hatching. Hum Reprod 1998; 13: 1331-5   DOI   ScienceOn
31 Primi M, Senn A, Montag M, Van der Ven H, Mandelbaum J, Veiga A. A European multicentre prospective randomized study to assess the use of assited hatching with diode laser and the benefit of an immunosuppressive/antibiotic treament in different patient population. Hum Reprod 2004; 19: 2325-33   DOI   ScienceOn