Browse > Article

Immunohistochemical Study on the Effect of Dexamethasone on the Luteolysis of Corpus Luteum of the Rat  

Park, Sun-Hee (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University)
Ko, Young-Bok (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University)
Rhee, Yun-Ee (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University)
Noh, Heung-Tae (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University)
Kim, Won-Sik (Depatment of Anatomy, College of Medicine, Chungnam National University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.35, no.1, 2008 , pp. 61-67 More about this Journal
Abstract
Objective: This study was attempted to look at the effect of dexamethasone on the luteolysis of corpus luteum in rats by immunohistochemical study. Methods: Counting with an optical microscope was conducted to make a comparison on difference in luteolysis and penetration of macrophage into three groups: control group of 30 female rats at 8 weeks of age, dexamethasone 0.1 mg administered group, and dexamethasone 1mg administered group. Results: As a result of TUNEL immunostaining, the percentage of luteolysis was significantly reduced in both dexamethasone 0.1 mg administered group and 1 mg administered group, and after ED1 immunostaining, macrophage invasion was reduced in dexamethasone 1 mg administered group. As a consequence of ED1 immunostaining, the immune response of macrophage was much decreased in dexamethasone 1 mg administered group than control group. Conclusion: Dexamethasone works on luteal cell, so it can suppress apoptosis. It can suppress luteolysis by suppression macrophage invasion into corpus luteum or suppress macrophage activation in corpus luteum.
Keywords
Dexamethasone; Corpus luteum; Luteolysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Niswender GD, Nett TM. Corpus luteum and its control in infraprimate species. In: Knobil E, Neill JD(eds.), The Physiology of Reproduction. Vol 1, 2nd ed. New York: Raven Press; 1994: 781-816
2 Parker CW. Neutrophil mechanisms. Am Rev Respir Dis 1991; 143: 59-60   DOI
3 Sasson R, Amsterdam A. Stimulation of apoptosis in human granulosa cells from in vitro fertilization patients and its prevention by dexamethasone: involvement of cell contact and bcl-2 expression. J Clin Endocrinol Metab 2002; 87: 3441-51   DOI
4 Sawyer HR, Niswender KD, Braden TD, Niswender GD. Nuclear changes in ovine luteal cells in response to PGF2 alpha. Domest Anim. Endocrinol 1990; 7: 229-37   DOI   ScienceOn
5 Kraal G, Shiamatey-Koolma R, Hoffer M, Baker D, Scheper R. Histochemical identification of guinea-pig macrophages by monoclonal antibody MR-1. Immunology 1988; 65: 523-8   PUBMED
6 Messmer UK, Winkel G, Briner VA, Pfeilschifter J. Glucocorticoids potently block tumour necrosis factor-alpha- and lipopolysaccharide-induced apoptotic cell death in bovine glomerular endothelial cells upstream of caspase 3 activation. Br J Pharmacol 1999; 127: 1633-40   DOI   ScienceOn
7 Messmer UK, Winkel G, Briner VA, Pfeilschifter J. Suppression of apoptosis by glucocorticoids in glomerular endothelial cells: effects on proapoptotic pathways. Br J Pharmacol 2000; 129: 1673-83   DOI   ScienceOn
8 Niswender GD, Juengel JL, McGuire WJ, Belfiore CJ, Wiltbank MC. Luteal function: The estrous cycle and early pregnancy. Biol Reprod 1994; 50: 239-47   DOI   ScienceOn
9 Yamamoto M, Fukuda K, Miura N, Suzuki R, Kido T, Komatsu Y. Inhibition by dexamethasone of transforming growth factor beta1-induced apoptosis in rat hepatoma cells: a possible association with Bcl-xL induction. Hepatology 1998; 27: 959- 66   DOI   ScienceOn
10 Nett TM, McClellan MC, Niswender GD. Effects of prostaglandins on the ovine corpus luteum: blood flow, secretion of progesterone and morphology. Biol Reprod 1976; 15: 66-78   DOI   ScienceOn
11 Hehnke KE, Christenson LK, Ford SP, Taylor M. Macrophage infiltration into the porcine corpus luteum during prostaglandin F2${\alpha}$ induced luteolysis. Biol Reprod 1994; 50: 10-5   DOI   ScienceOn
12 Niswender GD, Juengel JL, Silva PJ, Rollyson MK, McIntush ER. Mechanisms controlling the function and life span of the corpus luteum. Physiol Rev 2000; 80: 1-29   DOI   PUBMED
13 Schreiber JR, Nakamura K, Erickson GF. Rat ovary glucocorticoid receptor: identification and characterization. Steroids 1982; 39: 569-84   DOI   ScienceOn
14 Roby KF, Terranova PF. Localization of tumor necrosis factor (TNF) in the rat and bovine ovary using immunohistochemistry and cell blot: evidence for granulosa production. In: Hirshfield AN (ed.), Growth Factors and the Ovary. New York, Plenum Publishing Corporation 1989; 273-8
15 Rothchild I. The regulation of the mammalian corpus luteum. Recent Prog Horm Res 1981; 37: 183-298   PUBMED
16 Standaert FE, Zamora CS, Chew BP. Quantitative and qualitative changes in blood leukocytes in the porcine ovary. Am J Reprod Immunol 1991; 25: 163-8   DOI   PUBMED
17 Amsterdam A, Sasson R. The anti-inflammatory action of glucocorticoids is mediated by cell type specific regulation of apoptosis. Mol Cell Endocrinol 2002; 189: 1-9   DOI   ScienceOn
18 Kirsch TM, Friedman AC, Vogel RL, Flickinger GL. Macrophages in corpora lutea of mice: characterization and effects on steroid secretion. Biol Reprod 1981; 25: 629-38   DOI   ScienceOn
19 Bagavandoss P, Wiggins RC, Kunkel SL, Remick DG, Keyes PL. Tumor necrosis factor production and accumulation of inflammatory cells in the corpus luteum of pseudopregnancy and pregnancy in rabbits. Biol Reprod 1990; 42: 367-76   DOI   ScienceOn
20 Wen LP, Madani K, Fahrni JA, Duncan SR, Rosen GD. Dexamethasone inhibits lung epithelial cell apoptosis induced by IFN-gamma and Fas. Am J Physiol 1997; 273: 921-9
21 Nothnick WB, Pate JL. Interleukin-1 beta is a potent stimulator of prostaglandin synthesis in bovine luteal cells. Biol Reprod 1990; 43: 898-903   DOI   ScienceOn
22 Duncan WC, Rodger FE, Illingworth PJ. The human corpus luteum: reduction in macrophages during simulated maternal recognition of pregnancy. Hum Reprod 1998; 13: 2435-42   DOI   ScienceOn
23 Hurwitz A, Dushnik M, Solomon H, Ben-Chetrit A, Finci- Yeheskel Z, Milwidsky A, et al. Cytokine-mediated regulation of rat ovarian function: interleukin-1 stimulates the accumulation of a 92-kilodalton gelatinase. Endocrinology 1993; 132: 2709-14   DOI   ScienceOn
24 Zolti M, Meirom R, Shemesh M, Wollach D, Mashiach S, Shore L, et al. Granulosa cells as a source and target organ for tumor necrosis factor-$\alpha$. FEBS Lett 1990; 261: 253-5   DOI   ScienceOn
25 De Greef WJ, van der Schoot P. Effects of dexamethasone on ovarian activity in rats. Acta Endocrinol (Copenh) 1987; 116: 350-6   PUBMED
26 Hulboy DL, Rudolph LA, Matrisian LM. Matrix metalloproteinases as mediators of reproductive function. Mol Hum Reprod 1997; 3: 27-45   DOI   ScienceOn
27 Okuda K, Sakumoto R. Multiple roles of TNF super family members in corpus luteum function. Reprod Biol Endocrinol 2003; 95-104   DOI   ScienceOn
28 Gaytan F, Bellido C, Morales C, Sanchez-Criado JE. Both prolactin and progesterone are necessary for the induction of apoptosis in the regressing corpus luteum of the rat. Biol Reprod 1998; 59: 1200-6   DOI   ScienceOn
29 Azmi TI, O'Shea JD. Mechanism of deletion of endothelial cells during regression of the corpus luteum. Lab Invest 1984; 51: 206-17   PUBMED