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Expression of Endometriosis Related Genes in the Shed Endometrial Tissues from Menstrual Blood  

Park, Chan-Woo (Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Cheil General Hospital Kwandong University College of Medicine)
Jun, Jin-Hyun (Laboratory of Reproductive Biology & Infertility Cheil General Hospital Kwandong University College of Medicine)
Koong, Mi-Kyoung (Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Cheil General Hospital Kwandong University College of Medicine)
Song, In-Ok (Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Cheil General Hospital Kwandong University College of Medicine)
Publication Information
Clinical and Experimental Reproductive Medicine / v.34, no.4, 2007 , pp. 275-283 More about this Journal
Abstract
Objective: This study was performed to investigate the expressions of endometriosis related genes in shed endometrial tissues from menstrual blood of patients with or without endometriosis. Methods: The shed endometrial tissues were collected on 2$^{nd}$ or 3$^{rd}$ day of menstrual cycle with Wallace catheter in patients with endometriosis (n=16) and without endometriosis (n=26). The mRNA expressions of twelve kinds of endometriosis related genes were compared between two groups using semi-quantitative RT-PCR. Results: The collected shed endometrium was confirmed by histological observation. Expressions of telomerase, c-kit and aromatase mRNA were not detected by RT-PCR in shed endometrial tissues. The mRNA expressions of apoptosis related genes (fas, fas ligand, bcl-2, bax), stem cell factor, estrogen receptor-$\alpha$/$\alpha$, endometriosis protein-I and secretory leukocyte protease inhibitor gene were similar between shed endometrial tissues with endometriosis and without endometriosis. Conclusion: We could not find the difference of mRNA expressions of tested endometriosis related genes between shed endometrial tissues with or without endometriosis by semi-quantitative RT-PCR analysis. It may be related to the dynamical changes of gene expressions in the endometrium with menstrual cycle.
Keywords
Endometriosis; Shed endometrial tissues; mRNA expression; Semi-quantitative RT-PCR;
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