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Mitochondrial DNA Copy Number in the Patients of Korean Polycystic Ovary Syndrome (PCOS)  

Park, Ji-Eun ("Genome Research Center for Infertility and Reproductive Medicine" of Korea Ministry of Health & Welfare)
Jang, Min-Hee ("Genome Research Center for Infertility and Reproductive Medicine" of Korea Ministry of Health & Welfare)
Cho, Sung-Won ("Genome Research Center for Infertility and Reproductive Medicine" of Korea Ministry of Health & Welfare)
Kim, Yoo-Shin (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Won, Hyung-Jae (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Cho, Jung-Hyun (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Baek, Kwang-Hyun (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Lee, Sook-Hwan (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.33, no.4, 2006 , pp. 245-251 More about this Journal
Abstract
Objective: We analyzed quantification of mitochondria DNA (mtDNA) to investigate the relationship of mitochondria and pathogenesis of PCOS. Materials and Methods: Peripheral blood samples were collected from 28 patients with PCOS who were under the inclusion criteria for PCOS and from 28 healthy controls. Genomic DNA was used to analyze real-time PCR for mtDNA copy number quantification. The mtDNA copy number was compared between the control and PCOS groups. All data was expressed as mean ${\pm}$ SD. Statistical analysis was assessed by t-test. Results: In this study, the mtDNA $C_T$ was $11.67{\pm}0.422$ in PCOS patients and $11.51{\pm}0.722$ in control group, respectively. The mtDNA copy number was $1726410.71{\pm}407858.591$ the patients of in PCOS and $2167887.51{\pm}252459.28$ in control group (p=0.08), respectively. Conclusion: In our study, using real-time PCR, there was a tendency of lower mtDNA copy number in the patients of PCOS when comparing to the control group even though statistical difference was not significant. However, more extensive analysis is required to clarity relationship between mtDNA copy number and pathogenesis of PCOS.
Keywords
PCOS; Mitochondria; Real-time PCR;
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