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Role of Integrin, FAK (Focal Adhesion Kinase) and ERK (Extracellular Signal Regulated Kinase) on the Suppressed Cell Proliferation of Endometrial Cancer Cells by GnRH (Gonadotropin-Releasing Hormone)  

Choi, Jong Rak (Department of Clinical Pathology, Yonsei University)
Park, Dong Wook (Department of Molecular Science and Technology, Ajou University)
Choi, Dong Soon (Department of Molecular Science and Technology, Ajou University)
Min, Churl K. (Department of Molecular Science and Technology, Ajou University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.33, no.2, 2006 , pp. 115-123 More about this Journal
Abstract
Objective: To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II. Method: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [$^3H$] thymidine incorporation assay. Immunoblotting was utilized to detect proteins. Results: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin ${\beta}3$. Phosphorylation of FAK and ERK were induced by GnRH-I and -II. Conclusion: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.
Keywords
GnRH; Integrin; FAK; ERK; Phosphorylation;
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