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The Associtation between Methylenetetrahydrofolate Reductase Gene Polymorphisms and a Risk of Spontaneously Aborted Embryos  

Jeon, Nick-Beom (Department of Gynecology & Obstetrics, College of Medicine, Pochon CHA University)
Ji, Seung Il (Institute for Clinical Research, College of Medicine, Pochon CHA University)
Shin, Seung Joo (Department of Gynecology & Obstetrics, College of Medicine, Pochon CHA University)
Cha, Sun Hee (Department of Gynecology & Obstetrics, College of Medicine, Pochon CHA University)
Choi, Dong Hee (Department of Gynecology & Obstetrics, College of Medicine, Pochon CHA University)
Yim, Dong Jin (Institute for Clinical Research, College of Medicine, Pochon CHA University)
Park, Sang Hee (Genetics Lab. of Infertility Medical Center, CHA General Hospital)
Lee, Suman (Functional Genomics Lab. of CHA Research Institute, College of Medicine, Pochon CHA University)
Lee, Sang Hwa (Functional Genomics Lab. of CHA Research Institute, College of Medicine, Pochon CHA University)
Ko, Jung Jae (Institute for Clinical Research, College of Medicine, Pochon CHA University)
Kim, Nam Keun (Institute for Clinical Research, College of Medicine, Pochon CHA University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.33, no.1, 2006 , pp. 61-61 More about this Journal
Abstract
Objectives : This study was performed to understand the influence of the methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) genotypes on the spontaneously aborted embryos. Methods : DNA was extracted from tissue samples of 95 spontaneously aborted embryos and 100 samples of normal children randomly and 449 samples of normal adults were selected as the controls. MTHFR genotypes were determined by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results : The aborted embryo group had higher frequency of MTHFR 677CC type (p=0.014) and lower 677CT type (p=0.063) than the controlled child group. The frequency of MTHFR 677CT type was drastically lower than that of controlled adult group (p=0.032). In the MTHFR C677T/A1298C combination, 677CC/1298AC genotype of the aborted embryo was significantly higher (p=0.034) than that of controlled child group, but it was not statistically significant in controlled adult group (p=0.063). Conclusion : MTHFR 677CC and MTHFR 677CC/1298AC genotypes may represent genetic markers for the risk of spontaneously aborted embryos at least in Koreans.
Keywords
MTHFR; Abortus; Polymorphism; Homocysteine;
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