Browse > Article

mRNA Expression of Thrombospondin-1 and -2 in Severe Endometriosis Patients in Korean Women  

Hur, Sung Eun (Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University)
Lee, Ji Young (Department of Obstetrics and Gynecology, ollege of Medicine, KonKuk University)
Moon, Hye-Sung (Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University)
Chung, Hye Won (Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.32, no.3, 2005 , pp. 253-259 More about this Journal
Abstract
Objective: We investigated the expression of TSP-1 and -2 in eutopic endometrium of advanced endometriosis and control patients. Methods: The 33 endometriosis patients and 32 controls were enrolled. Endometrial samples were obtained from 65 premenopausal women aged 29-44 years, undergoing laparoscopic surgery or hysterectomy for non-malignant lesions. Sufficient samples were collected from 33 patients with endometriosis stage III and IV and 32 controls without endometriosis confirmed by laparoscopic surgery. The mRNA expression from eutopic endometrium for TSP-1 and -2 were analyzed by RT-QC PCR. Results: The mRNAs of TSP-1 and -2 were expressed in eutopic endometrium from endometriosis and normal controls throughout the menstrual cycle. There were no significant differences in expression of TSP-1 and TSP-2 in eutopic endometrium between controls and endometriosis patients. Conclusion: Our results indicated that TSP-1 and -2 had no crucial role compared to other molecules in the regulation of angiogenesis. These findings also suggest that dysregulation of other angiogenic regulators would be concerned in pathophysiologic role in endometriosis development.
Keywords
Thrombospondin; Endometriosis; Angiogenesis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Baenziger NL, Brodie GN, Majerus PW. A thrombinsensitive protein of human platelet membranes. Proc Natl Acad Sci USA 1971; 68: 240-243
2 Kyriakides TR, Zhu YH, Smith LT, Bain SD, Yang Z, Lin MT, et al. Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis. J Cell Biol 1998; 140: 419-30   DOI   ScienceOn
3 Iruela-Arispe ML, Bomstein P, Sage H. Thrombospondin exerts an antiangiogenic effect on cord formation by endothelial cells in vitro. Proc Natl Acad Sci USA 1991; 88: 5026-5030
4 Taylor RN, Mueller MD. Anti-angiogenic treatment of endometriosis: biochemical aspects. Gynecol Obstet Invest 2004; 57: 54-6
5 Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ, Holash J. Vascular-specific growth factors and blood vessel formation. Nature 2000; 407: 242-8   DOI   ScienceOn
6 Harada H, Nakagawa K, Saito M, Kohno S, Nagato S, Furukawa K, et al. Introduction of wild-type p53 enhances thrombospondin-1 expression in human glioma cells. Cancer Lett 2003; 191: 109-19   DOI   ScienceOn
7 Nap AW, Dunselman GA, Griffioen AW, Mayo KH, Evers JL, Groothuis PG. Angiostatic agents prevent the development of endometriosis-like lesions in the chicken chorioallantoic membrane. Fertil Steril 2005; 83: 793-5   DOI   ScienceOn
8 Sampson JA. Peritoneal endometriosis due to menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol 1927; 14: 422-69
9 Zhang X, Galardi E, Duquette M, Delic M, Lawler J, Parangi S. Antiangiogenic treatent with the three thrombospondin-l type 1 repeats recombinant protein in an orthotopic human pancreatic cancer model. Clin Cancer Res 2005; 11: 2337-44   DOI   ScienceOn
10 Simpson JL, Elias S, Malinak LR, ButtramVC Jr. Heritable aspects of endometriosis. I. Genetic studies. Am J Obstet Gynecol 1980; 137: 327-31
11 Li J, Zhou J, Tota B, Scalia G, Gattuso A. Regulating effects of vascular endothelial growth factor and ANG II on frog's pericardial stomata, mesothelium and angiogenesis. Chin Med Sci J 2001; 16: 23-8
12 Lawler J, Detrnar M. Tumor progression: the effects of thrombospondin-1 and -2 Int J Biochem Cell Biol 2004; 36: 1038-45   DOI   ScienceOn
13 Grossfeld GD, Ginsberg DA, Stein JP, Bochner BH, Esrig D, Groshen S, et al. Thrombospondin-l expression in bladder cancer: association with p53 alterations, tumor angiogenesis, and tumor progression. J Natl Cancer Inst 1997; 89: 219-27   DOI   ScienceOn
14 Sandberg T, Ehinger A, Casslen B. Paracrine stimulation of capillary endothelial cell migration by endometrial tissue involves epidermal growth factor and is mediated via up-regulation of the urokinase plasminogen activator receptor. J Clin Endocrinol Metab 2001; 86: 1724-30   DOI   ScienceOn
15 Kennedy S. Is therea genetic basisto endometriosis? Semin Reprod Endocrinol 1997; 15:309-18   DOI   PUBMED
16 Fontana A, Filleur S, Guglielmi J, Frappart L, BrunoBossio G, Boissier S, et al. Human breast tumors override the antiangiogenic effect of stromal thrombospondin-1 in vivo. Int J Cancer 2005; 116: 686-91   DOI   ScienceOn
17 Folkman J. Endogenous angiogenesis inhibitors. APMIS 2004; 112: 496-507   DOI   ScienceOn
18 Kyriakides TR, Zhu YH, Yang Z, Bomstein P. The distribution of the matricellular protein thrombospondin 2 in tissues of embryonic and adult mice. J Histochem Cytochem 1998; 46: 1007-15   DOI   ScienceOn
19 Kanda S, Shono T, Tomasini-Johansson B, Klint P, Saito Y. Role of thrombospondin-l-derived peptide, 4N1K, in FGF-2-induced angiogenesis. Exp Cell Res 1999; 252: 262-72   DOI   ScienceOn
20 Lobov IB, Brooks PC, Lang RA. Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo. Proc Natl Acad Sci USA 2002; 99: 11205-112010
21 Bums PA, Wilson DJ. Angiogenesis mediated by metabolites is dependent on vascular endothelial growth factor (VEGF). Angiogenesis 2003; 6: 73-7   DOI   ScienceOn
22 Esemuede N, Lee T, Pierre-Paul D, Sumpio BE, Gahtan V. The role of thrombospondin-l in human disease. J Surg Res 2004; 122: 135-42   DOI   ScienceOn
23 Rege TA, Fears CY, Gladson CL. Endogenous inhibitors of angiogenesis in malignant gliomas: nature's antiangiogenic therapy. Neuro-oncol 2005; 7: 106-21   DOI   ScienceOn
24 Adams RH, Wilkinson GA, Weiss C, Die1la F, Gale NW, Deutsch U, et al. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev 1999; 13: 295-306   DOI   ScienceOn
25 Iruela-Arispe ML, Dvorak HF. Angiogenesis: a dynamic balance of stimulator and inhibitors. Thrombo Haemost 1997; 78: 672-7
26 Nap AW, Griffioen AW, Dunselman GA, Bouma-Ter Steege JC, Thijssen VL, Evers JL, et al. Antiangiogenesis therapy for endometriosis. J Clin Endocrinol Metab 2004 Mar; 89: 1089-95   DOI   ScienceOn
27 Jung SP, Siegrist B, Wang YZ, Wade MR, Anthony CT, Homick C, et al. Effect of human Angiostatin protein on human angiogenesis in vitro. Angiogenesis 2003; 6: 233-40   DOI   ScienceOn
28 Thurston G. Role of Angiopoietins and Tie receptor tyrosine kinases in angiogenesis and lymphangiogenesis. Cell Tissue Res 2003; 314: 61-8   DOI   PUBMED   ScienceOn
29 American Society for Reproductive Medicine. Revised American Society for Reproductive Medicine classification of endometriosis. Fertil Steril 1997; 67: 817-21   DOI   PUBMED   ScienceOn
30 Berkkanoglu M, Arici A. Immunology and endometriosis. Am J Reprod Immunol 2003; 50: 48-59   DOI   PUBMED   ScienceOn
31 Noyes RW, Hertig AT, Rock J. Dating the endometrial biopsy. Am J Obstet Gynecol 1975; 122: 262-3