Browse > Article

Expression of doc-1 in Pregnant Uterus of the Mouse  

Cheon, Yong-Pil (Department of Biosciences, College of Veterinary Medicine, University of Illinois)
Publication Information
Clinical and Experimental Reproductive Medicine / v.29, no.4, 2002 , pp. 295-302 More about this Journal
Abstract
Uterine cells carry out proliferation and differentiation for preparation the embryonic implantation during pregnancy. Therefore regulation of the cell proliferation is an essential step for uterine preparation, but there is not much information about the proliferation related genes in pregnant uterus. To identify these implantation specific genes, a PCR-select cDNA subtraction method was employed and got a few genes. One of the identified genes is a novel gene encoding oral tumor suppressor doc-1. To detect the doc-1 expression on the pregnant uterus, dot blotting, RT-PCR, and in situ hybridization were employed. Dot blotting revealed that doc-1 mRNA expression increase after implantation. During normal pregnancy, doc-1 mRNA expression was detected as early as day 1 of pregnancy with RT-PCR. Its expression was increased about 15 times after embryonic implantation. doc-1 transcript was localized in luminal epithelial cells but it was very faint during preimplantation. After starting the implantation, it localized in the stromal cells; heightened expression of doc-1 correlates with intense stromal cell proliferation surrounding the implanting blastocyst on day 6 morning. However in the decidualized cells, the intensity of localized doc-1 mRNA was weak. From those results, it is revealed that doc-1 express at pregnant uterus of the mouse. In addition it is suggested that doc-1 is the gene regulating the proliferation of the luminal epithelial cells and stromal cells during early implantation and decidualization.
Keywords
Doc-1; Decidualization; Implantation; Proliferation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Croy BA, Chankakru S, Esadeg S, Ashkar AA, Wei Q. Decidual natural killer cells: key regulators of placental development (a review). J Reprod Immun 2002;57: 151-68   DOI   ScienceOn
2 Lim H, Paria BC, Das SK, Dinchuk JE, Langenbach R, Trzaskos JM, et al. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell 1997;91: 197-208   DOI   ScienceOn
3 Gordon HM, Kucera G, Salvo R, Boss JM. Tumor necrosis factor induces genes involved in inflammation, cellular and tissue repair, and metabolism in murine fibroblasts. J Immunol 1992; 148:4021-7   PUBMED
4 Chen D, Xu X, Zhu LJ, Angervo M, Li Q, Bagchi MK, et al. Cloning and uterus/oviduct-specific expression of a novel estrogen-regulated gene (ERGI). J BioI Chem 1999;274: 32215-24   DOI
5 Ansell JD, Barlow PW, McLaren A. Binucleate and polyploid cells in the deciduas of the mouse. J Embryol Experi Morphol 1974;31: 223-7
6 Chantakru S, Miller C, Roach LE, Kuziel WA, Maeda N, Wang WC, et al. Contri- butions from self-renewal and trafficking to the uterine NK cell population of earlypregnancy. J Immun 2002; 168: 22-8   DOI
7 Rider Y, Jones SR, Foster RT, ImakawaK. Changes in the temporal and spatial expression of H$\beta$58 during formation and maturation of the chorioallantoic placenta in the rat. BioI Reprod 2000; 63: 1735-46   DOI   ScienceOn
8 Tan J, Raja S, Davis MK, Tawfik O, Dey SK, Das SK. Evidence for coordinated interaction of cyclin D3 with p21 and cdk6 in directing the development of uterine stromal cell decidualization and polyploidy during implantation. Mech Devel 2002; 111:99-113   DOI   ScienceOn
9 Hatakeyama S, Jensen JP, Weissman AM. Subcellular localization and ubiquitin-conjugation enzyme (E2) interactions of mammalian HECT family ubiquitin protein. J BioI Chem1997; 272: 15085-92   DOI   ScienceOn
10 Xiao LJ, Chang H, Ding NZ, Ni H, Kadomatsu K, Yang ZM. Basigin expression and hormonal regulation in mouse uterus during peri-implantation period. Mol Reprod Dev 2002;63: 47-54   DOI   ScienceOn
11 Murray AW. Creative blocks: cell-cycle checkpoints and feedback controls. Nature 1992; 359:599-604   DOI   ScienceOn
12 Tsuji T, Duh FM, Latif F, Popescu NC, Zimonjic DB, McBride J, et al. Cloning, mapping, expression, function, and mutation analysis of the human ortholog of the hamster putative tumor suppressor gene doc-I. J BioI Chem 1998;2733: 6704-9
13 Ying Y, Zhao GQ. Detection of multiple bone morphogenetic protein messenger ribonucleic acidsand their signal transducer, Smad1, during mouse decidualization. BioI Reprod 2000; 63: 1781-9   DOI   ScienceOn
14 Shintani S, Ohyarna H, Zhang X, McBride J, Matsuo K, Tsuji T, et al. $p12^{DOC-1}$ is a novel cyclin-dependent kinase 2-associated protein. Mol Cell BioI 2000; 20: 6300-7   DOI
15 Ferenczy A. Regeneration of the human endometrium. In: Genoglio CM, Wolff LM, editor. Progress in surgical pathology. New York: Masson; 1980. p.157-77
16 Ben-Zimra M, Koler M, Melamed-Book N, Arensburg J, Payne AH, Orly J. Uterine and placental expression of steroidogenic genes during rodent pregnancy. Mol Cell Endo 2002; 187: 223-31   DOI   ScienceOn
17 Das SK, Lim H, Paria BC, Dey SK. Cyclin D3 in the mouse uterus is associated with the dediualization process during early pregnancy. J Mol Endocrinol 1999;22: 91-101   DOI   ScienceOn
18 Matsumoto H, Ma W, Dailoku T, Zhao X, Paria BC, Das SK, et al. Cyclooxygenase-2 differentially directs uterine angiogenesis during implantation in mice. J BioI Chem 2002; 277: 29260-7   DOI   ScienceOn
19 Benson GV, Lim H, Paria BC, Satokata I, Dey SK, Maas RL. Mechanisms of reduced fertility in Hoxa-10 mutant mice: uterine homeosis and loss of maternal Hoxa-10 expression. Development 1996; 122:2687-96   PUBMED
20 Robb L, Li R, Hartley L, Nandurkar HH, Koentgen F, Begley CG. Infertility in female mice lacking the receptor for interleukin 11 is due to a defective uterine response to implantation. Nat Med 1998;4:303-8   DOI   ScienceOn
21 Hunter T, Pines J. Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age. Cell 1994; 79:573-82   DOI   ScienceOn
22 Abrahamsohn PA, Zorn TMT. Implantation and decidualization in rodents. J Exp Zool 1993; 266: 603-28   DOI   ScienceOn
23 Reese J, Brown N, Das SK, Dey SK. Expression of neu differentiation factor during the periimplantation period in the mouse uterus. BioI Reprod 1998; 58: 719-27   DOI   ScienceOn
24 King RW, Deshaies RJ, Reters JM, Kirschner MW. How proteolysis drivesthe cell cycle. Science 1996;274: 1652-9   DOI   ScienceOn
25 Sherr CJ, Roberts JM. CDK inhibitors: positive and negative regulation of Gl-phase progression. Genes Dev 1999; 13: 1501-12   DOI   ScienceOn
26 Morgan DO. Principles of CDK regulation. Nature 1995;374: 131-4   DOI   ScienceOn
27 Leavitt WW. Cell biology of the endometrium. In: Wynn RM, Jollie WP, editor. Biology of the uterus. New York: Plenum Medical Book Company; 1989. p. 131-73
28 Sachs L, Shelesnyak MC. The development and suppression of polyploidy in the developing and suppressed deciduoma in the rat. J Endocrinol 1995; 12: 146-51   DOI   ScienceOn
29 Dey SK. Implantation. In: Adashi EY, Rock JA, Rosenwaks Z, editor. Reproductive endocrinology, surgery and technology. New York: Lippincott-Raven Publishers; 1996. p. 421-34
30 Pedykula HA, Coles LG, McCracken JA, King NW, Jr Longcope C, Kaiserman-Abramof IR. A zonal pattern of cell proliferation and differentiation in the rhesus endometrium duringthe estrogen surge. BioI Reprod 1984; 32: 1103-18
31 Shintani S, Mihara M, Terakado N, NakaharaY, Matsumura T, Kohno Y, et al. Reduction of $p12^{DOC-1}$ expression is a negative prognostic indicator in patients with surgically resected oral squamous cell carcinoma. Clin Cancer Res 2001; 7: 2776-82   PUBMED
32 Sherr CJ. Cancer cell cycles. Science 1996; 274:1672-7   DOI   ScienceOn
33 Finn CA, McLaren A. A study of the early stages of implantation in mice. J Reprod Fert 1967; 13: 259-67   DOI
34 Todd R, McBride J, Tsuji T, Donoff RB, Nagai M, Chou MY, et al. Deleted in oral cancer-I (doc-I), a novel oral tumor suppressor gene. FASEB J 1995; 9: 1362-70   DOI   PUBMED
35 Bany B, Schultz GA. Peiotrophin messenger ribonucleic acid levels in mouse endometrial stromal cells during decidualizaiton. Endocronology 2001; 142:955-8   DOI   ScienceOn