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Comparison of Sperm Morphology Evaluation Using Strict Criteria, Acrosome Reaction Following Ionophore Challenge and Zona-free Hamster Ova Sperm Penetration Assay as Prognostic Factors in Diagnosis of Male Infertility and In Vitro Fertilization  

Moon, Shin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Ryu, Buom-Yong (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
Pang, Myung-Geol (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
Oh, Sun-Kyung (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Lee, Jae-Hoon (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Suh, Chang-Suk (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Kim, Seok-Hyun (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Choi, Young-Min (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Kim, Jung-Gu (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Lee, Jin-Yong (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.29, no.1, 2002 , pp. 57-66 More about this Journal
Abstract
Objective : This study was designed to investigate the interrelationship and clinical usefulness of sperm morphology by strict criteria (SM), acrosome reaction following ionophore challenge test (ARIC) and sperm penetration assay (SPA) using zona-free hamster ova as prognostic factors in in vitro fertilization. Materials and Methods: Semen samples were provided by 83 patients undergoing IVF. We first evaluated the differences between normal fertilization group and poor fertilization group on three andrologic tests. Secondly, we analyzed the relationship between the three andrologic tests and in vitro fertilization on IVF settings. Finally, we evaluated the effectiveness of the three andrologic tests as the prognostic indicators for fertilizing ability. Results: The fertilization rate of all men in the poor fertilization group was less than 30%; but there was no evidence that this poor fertilization was due to oocyte defects. The results of three andrologic tests were significatly higher in normal fertilization group. Fertilization rate (%) in vitro was highly correlated (p<0.001) with % normal sperm by SM, ARIC value (%), and SPA result. By using Receiver-Operator-Characteristic curve (ROC), we evaluated the effectiveness of these three tests. The sensitivity and specificity of SM, ARIC test and SPA in predicting fertilization potential in IVF setting were 76% and 75%, 84% and 90%, and 76% and 95%, respectively. Conclusion: Our data suggest that the three andrologic tests can be reliable tools as prognostic factors of sperm fertilizing ability. Among these test, ARIC test and SPA gave more accurate information on fertilizing capacity. ARIC test was shown to have a predictive value for fertilizing ability comparable to that of SPA that appears to be a simple and cost-effective addition to current andrology laboratory. Combined application of these three tests may give more information on predicting sperm fertilizing capacity.
Keywords
Sperm; Strict morphology; ARIC; SPA; IVF;
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1 Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJD. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70: 219-28   DOI   PUBMED
2 신창재, 방명걸, 이진용, 장윤석, 정영채, 김창근. 햄스터 난자 침투 분석법에서 대조표준 정자로서 황소정자의 유용성에 관한 연구. 대한산부회지 1990; 33: 1758-63
3 Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehninger S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988; 49: 112-7   DOI   PUBMED
4 Fraser LR, Ahuja KK. Metabolic and surface events in fertilization. Gamete Res 1988; 491-519
5 Suarez SS, Wolf DP, Meizel S. Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid. Gamete Res 1986; 14: 107-21   DOI
6 Osman RA, Andria LA, Jones AD, Meizel S. Steroid-induced exocytosis: the human sperm acrosome reaction. Biochem Biophys Res Commun 1989; 160: 828-33   DOI   ScienceOn
7 Tesarik J. Comparison of acrosome reaction inducing activities of human cumulus oophorus, follicular fluid and ionophore A23187in human sperm populations of proven fertilizing ability in vitro. J Reprod Fertil 1985; 74: 383-8   DOI   PUBMED
8 Yovich JM, Edirisinghe WR, Yovich JL. Use of the acrosome reaction to ionophore challenge test in managing patients in an assisted reproduction program: a prospective, double-blind, randomized controlled study. Fertil Steril 1994; 61: 902-10   DOI   PUBMED
9 Ombelet W, Fourie F, Vandeput H, Bosmans E, Cox A, Janssen M, Kruger T. Teratozoospermia and in vitro fertilization: A randomized prospective study. Hum Reprod 1994; 9: 1979-84   DOI
10 Tesarik J. Appropriate timing of the acrosome reaction is a major requirement for the fertilizing spermatozoon. Hum Reprod 1989; 4: 957-61   DOI   PUBMED
11 Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, van Zyl JA, Smith K. Sperm morphological features as a prognostic factor in in vitro fertilization. Fertil Steril 1986; 46: 1118-23   DOI   PUBMED
12 신창재, 장윤석. 인간정자의 수정능력 부여 및 햄스터 난자 침투에 영향을 주는 인자들에 관한 연구. 대한산부회지 1990; 33: 954-75
13 Yanagimachi R, Yanagimachi H, Rogers BJ. The use of zona-free animal ova as a test system for the assessment of the fertilizing capacity of human spermatozoa Biol Reprod 1976; 15: 471-6
14 Cohen JC, Edwards R, Fehilly S, Fishel S, Hewitt J, Purdy J, Rowland G, Steptoe P, Webster J. In vitro fertilization: a treatment for male infertility. Fertil Steri1 1985; 43: 422-32   DOI
15 Oehninger S, Coddington CC, Scott R, Franken DA, Burkman LJ, Acosta AA, Hodgen GD. Hemizona assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome. Fertil Steril 1989; 51: 665-70   DOI   PUBMED
16 Koukoulis GN, Vantman DJ, Dennison L, Banks SM, Sherins RJ. Low acrosin activity in a subgroup of men with idiopathic infertility does not correlate with sperm density, percent motility, curvilinear velocity, of linearity. Fertil Steril 1989; 52: 20-127
17 Henkel R, Muller C, Miska W, Gips H, Schill WB. Determination of the acrosome reaction in human spermatozoa is predictive of fertilization in vitro. Hum Reprod 1993; 8: 2128-32   DOI   PUBMED
18 Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde MP, Van Assche E, Devroey P. Higher success rate by intracytoplasmic sperm injection than by subzonal insemination: Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993; 8: 1055-60   DOI   PUBMED
19 방명걸, 오선경, 신창재, 문신용, 이진용, 장윤석. Sperm Penetration Assay의 임상적 타당성에 관한 연구. 대한불임학회지 1993; 20: 1-7   과학기술학회마을
20 Cockett ATK, Netto ICV, Dougherty KA, Urry RL. Semen analysis: a review of samples from 225 men seen at an infertility clinic. J Urol 1975; 114: 560-3   DOI
21 De Jonge CJ, Han HL, Lawrie H, Mack SR, Zaneveld LJD. Modulation of human sperm acrosome reaction by effectors of adenylate cyclase/cyclic AMP second-messenger pathway. J Exp Zool 1991; 258: 113-25   DOI   ScienceOn
22 Palermo G, Cohen J, Alikani M, Adler A, Rosenwaks Z. Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil Steril 1995; 63: 1231-40   DOI   PUBMED
23 Hargreave TB, Elton RA. Fecundability rates from an infertile population. Br J Uro 1986; 58: 194-7   DOI   ScienceOn
24 Calvo L, Vantman D, Banks SM, Tezon J, Koukoulis GN, Dennison L, Sherins RJ. Follicular fluid-induced acrosome reaction distinguishes a subgroup of men with unexplained infertility not identified by semen analysis. Fertil Steril 1989; 52: 1048-54.   DOI   PUBMED
25 Cross NL, Morales P, Overstreet JW, Hanson FW. Two simple methods for detecting acrosome-reacted human sperm. Gamete Res 1986; 15: 213-26. 20. Fraser LR, Ahuja KK. Metabolic and surface events in fertilization. Gamete Res 1988; 491-519
26 Johnson A, Bassham B, Lipshultz LI, Lamb DJ. Methodology for the optimized sperm penetration assay. In: Keel BA, Webster BW, eds. Handbook of the laboratory diagnosis and treatment of infertility. Boca Raton, Ann Arbor, Boston: CRC Press. 1990; 7: 135-47
27 이용빈. 소의 인공수정. 가축인공수정요론. 서울: 선진문화사, 1984; 4: 106
28 Calvo L, Dennison-Lagos L, Bank SM, Dorfmann A, Thorsell L, Bustillo M, Schulman JD, Sherins RJ. Acrosome reaction inducibility predicts fertilization success at in-vitro fertilization. Hum Reprod 1994; 9: 1880-6   DOI   PUBMED
29 Cummins JM, Pember SM, Jequier AM, Yovich JL. A test of the human sperm acrosome reaction following ionophore challenge. J Androl 1991; 12: 98-103   PUBMED
30 Liu DY, Baker HWG. Test of human sperm function and fertilization in vitro. Fertil Steril 1992; 58: 465-83   DOI   PUBMED