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http://dx.doi.org/10.6116/kjh.2022.37.6.1.

Study on Chondro-protective and Anti-inflammatory Effects of Caraganae Sinicae Flos Extract  

Park, Dongjun (LG Household and Healthcare ltd)
Lee, Hong Gu (LG Household and Healthcare ltd)
Ko, Chung Ho (Garden and Plant Resources Division, Korea National Arboretum)
Park, Hyoungkook (LG Household and Healthcare ltd)
Jin, Mu Hyun (LG Household and Healthcare ltd)
Cho, Ho Song (LG Household and Healthcare ltd)
Publication Information
The Korea Journal of Herbology / v.37, no.6, 2022 , pp. 1-8 More about this Journal
Abstract
Objectives : This research aimed to investigate chondro-protective and anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract and its compound, tilianin. Methods : Caraganae Sinicae Flos was extracted with 50% ethanol. Tilianin in Caraganae Sinicae Flos 50% ethanol extract was quantified by HPLC analysis method. To investigate chondro-protective effects of Caraganae Sinicae Flos 50% ethanol extract, ATDC5 chondrogenic cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and tumor necrosis factor-𝛼 (TNF𝛼) for 24 hours. After treatement for 24 hours, media supernatant was used for quantifying protein level of matrix metalloproteinase-3 (MMP3) by ELISA and harvested cells were used for analyzing mRNA expression level of matrix metalloproteinase-13 (MMP13) by reverse transcription PCR. To identify anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract, RAW 264.7 macrophage cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and lipopolysaccharide (LPS) for 24 hours. media was used for quantifying the level of prostaglandin E2 (PGE2), interleukin-6 (IL6) by ELISA and nitric oxide by Griess reagent asssay. Results : Caraganae Sinicae Flos 50% ethanol extract and tilianin attenuated protein level of MMP3 and mRNA expression level of MMP13 in TNF𝛼-activated ATDC5 cells. Caraganae Sinicae Flos 50% ethanol extract inhibited the level of PGE2, IL6 and NO in LPS-activated RAW 264.7 cells in dose dependent manner, though tilianin inhibited PGE2 only. Conclusions : These results presented that Caraganae Sinicae Flos 50% ethanol extract could be used as natural medicines for osteoarthritis.
Keywords
Caraganae Sinicae Flos; Tilianin; Chondro-protective effects; Anti-inflammatory Activities;
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1 Lu J, Zhang H, Cai D, Zeng C, Lai P, Shao Y, et al. Positive feedback regulation of subchondral H-type vessel formation by chondrocyte promotes osteoarthritis development in mice. J Bone Miner Res. 2018 ; 33 : 909-20.   DOI
2 Kim TY, Kim JH, Park JH, Lee JK, Han C, Kum CJ, Oh JW. The Domestic Trends of Traditional Korean Medicine Treatments on Degenerative Knee Arthritis. Journal of Korea CHUNA Manual Medicine. 2014 ; 9(2) : 69-79.
3 Jang JH, Kim KH. A Study on Osteoarthritis of the Knee. J Acupunct Res. 1998 ; 15(2) : 493-509.
4 Bae GC, Bae DY. The anti-inflammatory effects of ethanol extract of Allium Hookeri cultivated in South Korea. Kor. J. Herbol. 2012 ; 27(6) : 55-61.   DOI
5 Yoo JH, Doh ES, Chang JP, Kil KJ. Antioxidant Activities of Hot Water and Ethanol Extracts from the stem of Sorbus commixta Heal. Kor. J. Herbol. 2017；32(3):29-36.   DOI
6 KIM TJ. Korean resources plants II. Seoul : Seoul National University press. 1997 : 11.
7 Ma CW, Ham I, Whang WK. The flavonoids from the Caragana chamlagu leaves. Yakhak Hoechi. 1999 ; 43(2) : 143-9.
8 Qi JB, Shu N, Ma DY, Hu CQ. Isoflavonoid compounds from roots of Caragana sinica. Zhongguo Tianran Yaowu. 2007 ; 5 : 101-4.
9 Robinson WH, Lepus CM, Wang Q, Raghu H, Mao R, Lindstrom TM, Sokolove J. Low-grade inflammation as a key mediator of the pathogenesis of osteoarthritis. Nat Rev Rheumatol. 2016 ; 12(10) : 580-92.   DOI
10 Li T, Zou QP, Huang F, Cheng GG, Mao ZW, Wang T, Dong FW, Li BJ, He HP, Li YP. Flower extract of Caragana sinica. ameliorates DSS-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model. Iran J Basic Med Sci. 2021 ; 24(5) : 595-603.
11 Park YK. Inhibitory effects of Zanthoxylum piperitum on the LPS-induced production of nitric oxide and proinflammatory cytokines in RAW264.7 cells. Kor. J. Herbol. 2006 ; 21(4) : 69-76.
12 Donell S. Subchondral bone remodelling in osteoarthritis. EFORT Open Rev. 2019 ; 4(6) : 221-29.   DOI
13 Cui Z, Crane J, Xie H, Jin X, Zhen G, Li C, et al. Halofuginone attenuates osteoarthritis by inhibition of TGF-beta activity and H-type vessel formation in subchondral bone. Ann Rheum Dis. 2016 ; 75 : 1714-21.   DOI
14 Hugle T, Geurts J. What drives osteoarthritis - synovial versus subchondral bone pathology. Rheumatology. 2017 ; 56(9) : 1461-71.   DOI
15 Chae YA, et al. Medicinal plant biology. Seoul : Hyangmunsa CO. 2007 : 1.
16 Zhang LP, Ju MH, Hu CQ. Studies on isoflavones from Chinese peashrub (Caragana sinica). Chinese Traditional Herbal Drugs. 1994 ; 24 : 134-6.
17 Sokolove J, Lepus CM. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Ther Adv Musculoskelet Dis. 2013 ; 5(2) : 77-94.   DOI
18 Lees S, Golub SB, Last K, Zeng W, Jackson DC, Sutton P, Fosang AJ. Bioactivity in an Aggrecan 32-mer fragment is mediated via toll-like receptor 2. Arthritis Rheumatol. 2015 ; 67(5) : 1240-9.   DOI
19 Latourte A, Cherifi C, Maillet J, Ea HK, Bouaziz W, Funck-Brentano T, Cohen-Solal M, Hay E, Richette P. Systemic inhibition of IL-6/Stat3 signalling protects against experimental osteoarthritis. Ann Rheum Dis. 2017 ; 76(4) : 748-55.   DOI
20 Wang S, Ma D, Hu C. Three new compounds from the aerial parts of Caragana sinica. Helvetica Chimica Acta. 2005 ; 88 : 2315-21.   DOI
21 Klatt AR, Paul-Klausch B, Klinger G, Kuhn G, Renno JH, Banerjee M, Malchau G, Wielckens K. A critical role for collagen II in cartilage matrix degradation: collagen II induces pro-inflammatory cytokines and MMPs in primary human chondrocytes. J Orthop Res. 2009 ; 27 : 65-70.   DOI
22 Lambert C, Borderie D, Dubuc JE, Rannou F, Henrotin Y. Type II collagen peptide Coll2-1 is an actor of synovitis. Osteoarthr Cartil. 2019 ; 27 : 1680-91.   DOI
23 Rubartelli A, Lotze MT. Inside, outside, upside down: damage-associated molecular-pattern molecules (DAMPs) and redox. Trends immunol. 2007 ; 28(10) : 429-36.   DOI
24 Zhang H, Cai D, Bai X. Macrophages regulate the progression of osteoarthritis. Osteoarthritis Cartilage. 2020 ; 28(5) : 555-61.   DOI
25 Zhang Y, Fu Z. Role of tilianin against acute lung injury in In vitro LPS-induced alveolar macrophage cells and an in vivo C57BL/6 mice model. J Environ Pathol Toxicol Oncol. 2020 ; 39(4) : 335-44.   DOI
26 Zhang H, Wang H, Zeng C, Yan B, Ouyang J, Liu X, et al. mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis. Osteoarthritis Cartilage. 2017 ; 25(6) : 952-63.   DOI
27 Jeon YS, Jo BS, Park HJ, Kang SA, Cho YJ. Screening of Biological Activity of Caragana sinica Extracts. J Korean Soc Food Sci Nutr. 2012 ; 41(9) : 1211-9.   DOI