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http://dx.doi.org/10.6116/kjh.2022.37.5.27.

3-Acetyl-11-Keto-Beta-Boswellic Acid from Boswellia serrata Attenuates Monosodium Iodoacetate-induced Osteoarthritis by Chondroprotective and Anti-inflammatory Effects  

Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Choi, Hak Joo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Hae-Jin (DHU Bio Convergence Testing Center)
Choi, Hwang-Yong (Ju Yeong NS Co. Ltd.)
Kim, Hwa-Young (Ju Yeong NS Co. Ltd.)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.37, no.5, 2022 , pp. 27-35 More about this Journal
Abstract
Objectives : 3-Acetyl-11-keto-𝛽-boswellic acid (AKBA) is a major active compound in Boswellia serrata. We investigated the arthritic changes following AKBA administration in monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : All rats were randomly divided into five groups: Normal, Control, INDO (indomethacin 2 mg/kg treated), AKBA30 (AKBA 30 mg/kg treated), and AKBA60 (AKBA 60 mg/kg treated); drugs were given 2 weeks before MIA injection. For all groups except the normal group, 50 µL of sterile saline with MIA (80 mg/mL) was injected into the right knee joint 2 weeks after drug administration. The drug administration was continued for 4 weeks from 1 week after osteoarthritis induction. The histomorphological changes of knee joint cartilage were observed by H&E staining. Also, the levels of glycosaminoglycan (GAG), cartilage oligomeric matrix protein (COMP), 5-lipoxygenase (5-LOX), 5-LOX-activating protein (FLAP), and leukotriene B4 (LTB4) in the knee joint were determined by the ELISA kits. The expressions of mitogen-activated protein kinases (MAPKs), inflammatory cytokines, and matrix metalloproteinases (MMPs) in knee joint were detected by Western blot. Results : Data show that levels of 5-LOX, FLAP, LTB4, and COMP were downregulated significantly in the AKBA treated groups when compared to those in the Control group. On the other hand, GAG levels were significantly elevated. As a result of Western blot, the AKBA-treated groups significantly inhibited phosphorylation of MAPKs. In addition, significant downregulation of the expression of inflammatory cytokines and MMPs was found in the AKBA-treated groups. Conclusion : Our findings suggest that administration of AKBA could exert better chondroprotective and anti-inflammatory effects for MIA-induced osteoarthritis rats.
Keywords
Boswellia serrata; 3-Acetyl-11-keto-${\beta}$-boswellic acid; Monosodium iodoacetate; Osteoarthritis;
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