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http://dx.doi.org/10.6116/kjh.2022.37.5.17.

Combined Treatment of Silymarin and Jakyakgamcho-tang Suppresses Hepatic Lipid Accumulation and Inflammation in C57BL/6 Mice  

Choi, Jeong Won (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Cho, Su-Jung (Gyeongbuk Institute for Regional Program Evaluation)
Shin, Mi-rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Hae-Jin (DHU Bio Convergence Testing Center, Daegu Hanny University Republic of Korea)
Publication Information
The Korea Journal of Herbology / v.37, no.5, 2022 , pp. 17-26 More about this Journal
Abstract
Objective : The aim of the present study is to examine hepatic lipid-lowering and anti-inflammatory effects of silymarin combined with Jakyakgamcho-tang on non-alcoholic fatty liver disease in a high fat diet-induced obese mice model. Methods : C57BL/6 mice were divided into four dietary groups: (1) Normal, (2) Control (60% high-fat diet), (3) Control + silymarin 50 mg/kg/day (Silymarin), (4) Control + Silymarin 50 mg/kg/day + Jakyakgamcho-tang 100 mg/kg/day (SPG). After 12 weeks administration, mice were sacrificed and lipids and inflammation-related biomarkers were analyzed liver and plasma. Results : Silymarin and SPG treatments significantly lowered body and liver weights compared to the Control. Serumlipids (triglyceride (TG), total cholesterol) and pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin 1𝛽, and IL-6) concentrations were significantly lowered in the Silymarin and SPG groups than the Control group. Silymarin and SPG treatments suppressed hepatic TG level and hepatic lipid droplets compared to the Control. Theses two treatments significantly increased hepatic kinase B1 and AMP-activated protein kinase protein levels, and significantly decreased hepatic key lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase and stearyl coenzyme A desaturase 1) protein levels than the Control. SPG also significantly increased hepatic fatty acid oxidation-related protein (peroxisome proliferator-activated receptor alpha and uncoupling protein 2) levels than the Control. Conclusions: Silymarin and SPG suppressed hepatic lipid accumulation by regulating hepatic protein expression, and lowered blood pro-inflammatory cytokines concentrations though the synergic effect of silymarin and Jakyakgamchotang was not clear.
Keywords
silymarin; Jakyakgamcho-tang; hepatic lipid accumulation; inflammation; C57BL/6 mice;
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1 Guo Y, Wang S, Wang Y, Zhu T. Silymarin improved diet-induced liver damage and insulin resistance by decreasing inflammation in mice. Pharmaceutical Biology. 2016;54(12):2995-3000.   DOI
2 Yoon HJ, Park YS. Effects of Scutellaria baicalensis Water Extract on Lipid Metabolism and Antioxidant Defense System in Rats Fed High Fat Diet. J Korean Soc Food Sci Nutr, 2010;39(2):219-226   DOI
3 Jeong CS, Jung KW, Jeong JS. Hepatoprotective Effect of Subfractions of Carthamus tinctorius L. Semen on the Reversal of Biotransformation Enzyme Activities in CCl4-induced Hepatotoxic Rats. J. Fd Hyg. Safety, 1999;14(2):172-178.
4 Gou S, He M, Li B, Zhu N, Ni J. Hepatoprotective effect of total flavonoids from Glycyrrhiza uralensis Fisch in liver injury mice. Natural product research. 2021;35(24):6083-6087.   DOI
5 Park SH, Plank LD, Suk KT, Park YE, Lee J, Choi JH, Heo NY, Park J, Kim TO, Moon YS, Kim HK, Jang HJ, Park HY, Kim DJ. Trends in the prevalence of chronic liver disease in the Korean adult population, 1998-2017. Clin Mol Hepatol. 2020;26(2):209-215.   DOI
6 Ni X, Wang H. Silymarin attenuated hepatic steatosis through regulation of lipid metabolism and oxidative stress in a mouse model of nonalcoholic fatty liver disease (NAFLD). Am J Transl Res. 2016;8(2):1073-1081.
7 Sung WY, Yoon GR, Jang SM. 2000. Comparison of effective constituents of Korean paeony roots (Paeoniae radix) cultivated in different regions. Kor Soc Postharvest Sci & Technol 7: 297-301.
8 Ro HS, Ko WK, Yang HO, Park KK, Cho YH, Lee YE, Park HS. 1999. Isolation of antihyperlipidemic substances from methanol extract of Paeoniae radix. J Kor Pharm Sci 29:55-60
9 Li YC, Qiao JY, Wang BY, Bai M, Shen JD, Cheng YX. Paeoniflorin ameliorates fructose-induced insulin resistance and hepatic steatosis by activating LKB1/AMPK and AKT pathways. Nutrients. 2018;10(8):1024.   DOI
10 Feher J, Lengyel G. Silymarin in the prevention and treatment of liver diseases and primary liver cancer. Current Pharmaceutical Biotechnology. 2012;13(1):210-217.   DOI
11 Kim CM. The Korean Association for the study of the Liver (KSAL). KASL clinical practice guidelines: Management of nonalcoholic fatty liver disease. Clin Mol Hepatol. 2013; 19(4):325-348.   DOI
12 Xiao J, So KF, Liong EC, Tipoe GL. Recent advances in the herbal treatment of non-alcoholic fatty liver disease. J Tradit Complement Med. 2013;3(2):88-94.   DOI
13 Shin SW, Yoon EK, Jo SH, Hawang JH. A Study on the 'Harmonizing All Medicinals' Property of Gancao. J Korean Med Classics 2020:33(1):179-196
14 Lee EH, Baek SY, Kim KY, Lee SG, Kim SC, Lee HS, Kim YW. Effect of Rheum undulatum Linne extract and Glycyrriza uralensis Fischer extract against arachidonic acid and iron-induced oxidative stress in HepG2 cell and CCl4-induced liver injury in mice. Herbal Formula Science. 2016;24(3):163-174.   DOI
15 Sung YY, Yuk HJ, Kim DS. Comparison of ingredient quantities and anti-fatigue effects of Jakyakgamcho-Tang according to extraction solvent. Kor J Herbol. 2020;35(2):31-38.
16 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199-1200.   DOI
17 Kim BW. Anti-inflammatory effect of Jakyakgamcho-tang. Korean J Orient Int Med. 2010;31(2):365-371.
18 Folch J, Mee L, Stanley GSH. A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem. 1975;226(1):497-509.   DOI
19 Lee SH, Jung JY, Park SM, Jegal KH, Byun SH, Cho IJ, Kim SC, Kim KJ, Kim YW. Hepatoprotective effect of Paeoniae radix via Nrf2 activation. Kor J Herbol. 2016;31(1):33-40.   DOI
20 Feng B, Meng R, Huang B, Shen S, Bi Y, Zhu D. Silymarin alleviates hepatic oxidative stress and protects against metabolic disorders in high-fat diet-fed mice. Free Radical Research. 2016; 50(3): 314-327.   DOI
21 Gu M, Zhao P, Huang J, Zhao Y, Wang Y, Li Y, Li Y, Fan S, Ma YM, Tong Q, Yang L, Ji G, Huang C. Silymarin ameliorates metabolic dysfunction associated with diet-induced obesity via activation of Farnesyl X Receptor. Front Pharmacol. 2016;7:345.
22 Dimiter A, Valentin AP, Kevin JT, Leonid P, Characterization of inflammation and insulin resistance in high-fat diet-induced male C57BL/6J mouse model of obesity. Animal Model Exp Med. 2019;2:252-258   DOI
23 Kim SB, Kang OH, Lee YS, Han SH, Ahn YS, Cha SW, Seo YS, Kong R, Kwon DY. Hepatoprotective effect and synergism of bisdemethoycurcumin against MCD diet-induced nonalcoholic fatty liver disease in mice. PLoS One. 2016;24(1):142-142.
24 Plaa GL, Charbonneau M. Detection and evaluation of chemically induced liver injury. In Principles and Methods of Toxicity, 4th ed. Philadelphia : Taylor and Francis. 2001:1145-98
25 Skottova N, Krecman V. Silymarin as a potential hypocholesterolamic drug. Physiol. Res. 1998;47:1-7.
26 Colica C, Boccuto L, Abenavoli L. Silymarin: An option to treat non-alcoholic fatty liver disease. World J Gastroenterol. 2017;23(47):8437-8438.   DOI
27 Feng R, Chen JH, Liu CH, Xia FB, Xiao Z, Zhang X, Wan JB. A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice. Phytomedicine. 2019;58:152824.   DOI
28 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231-1237.   DOI
29 Kim YS, Yuk HJ, Kim DS. Comparison of efficacies of different Jakyakgamcho-tang extracts on H2O2-induced C2C12 cell viability. Preprints. 2020; 080130.