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http://dx.doi.org/10.6116/kjh.2022.37.3.9.

Effect of Angelicae Gigantis Radix for Inflammatory Response in HaCaT Cells  

Huh, Jung (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Park, Hoyeon (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Eom Ji (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Eun-Young (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.37, no.3, 2022 , pp. 9-19 More about this Journal
Abstract
Objectives : Angelicae Gigantis Radix (AG) is a plant of the Ranunculus family. AG have been reported to have various pharmacological effects on human health which include uterine growth promotion, anti-inflammatory, analgesic, and immune enhancement. However, research on dermatitis disease is insufficient. Therefore, we investigated the effects of AG on tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ) stimulated HaCaT cell. Methods : To investigate the effect of AG on HaCaT cell, HaCaT cells were pre-treated with AG for 1 hour and then stimulated with TNF-α/IFN-γ. After 24 hours, media and cells were harvested to analyze the inflammatory mediators. Concentration of human interleukin-1beta (IL-1β), monocyte chemoattractant protein-1 (MCP-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), and TNF-α in the media were assessed by ELISA. mRNA expression of human thymus and activation-regulated chemokine (TARC), IL-6, and IL-8 were analyzed by RT-PCR. Additionally, the mechanisms of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway were investigated by Western blot. Results : The treatment of AG inhibited gene expression levels of IL-6, IL-8, and TARC and protein expression levels of IL-1β, MCP-1, and GM-CSF. Also, AG significantly reduced extracellular signal-regulated kinase (ERK) phosphorylation and NF-κB translocation in TNF-α/IFN-γ stimulated HaCaT cell. Conclusions : Taken together, these results demonstrate that AG can alleviate inflammatory diseases such as atopic dermatitis by regulating the expression of inflammatory cytokines. Also, it suggest that AG may a promising candidate drug for the treatment of inflammatory disease such as atopic dermatitis.
Keywords
Angelicae Gigantis Radix; HaCaT cell; Atopic dermatitis; Cytokine; MAPKs;
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Times Cited By KSCI : 3  (Citation Analysis)
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