Browse > Article
http://dx.doi.org/10.6116/kjh.2022.37.3.29.

Respiratory Protective Effect of a RML on PM10D-induced Lung Injury Mouse Model  

Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University)
Park, Hae-Jin (DHU Bio Convergence Testing Center, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.37, no.3, 2022 , pp. 29-39 More about this Journal
Abstract
Objective : This study is aimed to evaluate the protective effects of Rehmanniae Radix, Mori Folium, and Liriopie Tuber mixture (RML) on lung injury of Particulate matter less than 10 um in diameter and diesel exhaust particles (PM10D) mice model. Methods : To investigate the anti-inflammatory activity of RML, PM10D was diluted in aluminum hydroxide (Alum) in 7-week-old male mice and induced by Intra-Nazal-Tracheal (INT) injection method. Animal experiments were divided into 5 groups. Nor (normal mice), CTL (PM10D-induced mice with the administration of distilled water), DEXA (PM10D-induced mice with the administration of 3 mg/kg Dexamethasone), RML 100 (PM10D-induced mice treated with RML 100 mg/kg weight), and RML 200 (PM10D-induced mice treated with RML 200 mg/kg body weight). After 11 days administration, mice were sacrificed and inflammation-related immune cells in broncho-alveolar lavage fluid (BALF) were analyzed. Inflammation-related biomarkers were also analyzed in blood and lungs. Lung tissue was observed through histological examination. Results : In the PM10D induced model, the PML showed decreases in CXCL-1 and IL-17A in BALF. Expression of inflammatory cytokines and cough-related mRNA genes was significantly decreased in serum and lung tissue. The mixture treatment of RML significantly improved the immune related cells in the serum. In addition, histological observations showed a tendency to decrease the severity of lung injury. Conclusions : Overall, these results confirmed the respiratory protective effect of the RML mixture in a model of lung injury induced by air pollution (PM10+DEP), suggesting that it is a potential treatment for respiratory damage.
Keywords
PM10-D; Lung Injury; Rehmanniae Radix; Mori Folium; Liriopis Tuber;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Ahn JH. Effect of Liriopis tuber on Liver function activity. Department of Pharmacy, Catholic University of Daegu. 2000.
2 Choi SB, Wha JD, Park S. The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway. Life Sci. 2004 ; 75(22) : 2653-64.   DOI
3 Yoshitaka I. Research on the quality distribution of JIS type-II fly ash in Japan. World of Coal Ash. www.flyash.info/2007/67ishikawa.pdf (cited by 2007 May 7).
4 Lim HB, Kim SH. The effect of crude saponins of Korean red ginseng against airway inflammation and airway hyperresponsiveness induced by diesel exhaust particles in mice. Korean Journal of Medicinal Crop Science. 2009 ; 17 : 90-6.
5 Grace MS, Baxter M, Dubuis E, Birrell MA, Belvisi MG. Transient receptor potential (TRP) channels in the airway: role in airway disease. Br J Pharmacol. 2014 ; 171 : 2593-607.   DOI
6 Park MK, Seong MG, Lee MK. Effects of TRPV1 in formalin-induced nociceptive behavior in theorofacial area of rats. Journal of the Korea Academia-Industrial cooperation Society. 2014 ; 15(1) : 316-22.   DOI
7 Traves SL, Donnelly LE. Th17 cells in airway diseases. Curr Mol Med. 2008 ; 8(5) : 416-26.   DOI
8 Son HJ, Kim YS, Misaka T, Noh BS, Rhyu MR. Activation of the Chemosensory Ion Channels TRPA1 and TRPV1 by Hydroalcohol Extract of Kalopanax pictus Leaves. Biomol Ther. 2012 ; 20(6) : 550-5.   DOI
9 Kwon YD, Song YS. Effects of Rhizoma rehmanniae adminstration on the Innate and Adap tive Responses in the Mouse. j. of Oriental Rehabilitation Medicine. 1998 ; 8(1) : 233-54.
10 Park YB, Rhee CK, Yoon HK, Oh YM, Lim SY, Lee JH, Yoo KH, Ahn JH. COPD clinical practice guideline of the Korean Academy of Tuberculosis and Respiratory Disease: a summary. Tuberc Respir Dis (Seoul). 2018 ; 81(4) : 261-73.   DOI
11 Doeing DC, Solway J. Airway smooth muscle in the pathophysiology and treatment of asthma. Journal of Applied Physiology. 2013 ; 114 : 834-43.   DOI
12 Hoffmann B, Weinmayr G, Hennig F, Fuks K, Moebus S, Weimar C, Dragano N, Hermann DM, Kalsch H, Mahabadi AA, Erbel R, Jockel KH. Air quality, stroke, and coronary events: Results of the heinz nixdorf recall study from the ruhr region. Deutsches Arzteblatt International. 2015 ; 112 : 195-201.
13 Bohadana AB, Massin N, Wild P, Toamain JP, Engel S, Goutet P. Symptoms, airway responsiveness, and exposure to dust in beech and oak wood workers. Occupational and Environmental Medicine. 2000 ; 57 : 268-73.   DOI
14 Kim JS. Effect of Rhemanniae Radix on the Hyperglycemic Mice Induced with Streptozotocin. The Korean Society of Food Science and Nutrition. 2004 ; 33(7) : 1133-8.   DOI
15 Kim TH, Yang WK, Lee SW, Kim SH, Lyu YR, Park YC. Inhibitory Effects of GGX on Lung Injury of Chronic Obstructive Lung Disease (COPD) Mice Model. J Korean Med. 2021 ; 42(3) : 56-71.   DOI
16 Chae BS, Shin TY. Effect of Fresh Rehmanniae Radix Methanol Extracts on the Production of Cytokines. Yakhak Hoeji. 2006 ; 50(1) : 184-90.
17 Herbology editorial committee of Korean medicine schools. Herbology [Boncho-hak]. Seoul : Young-Lim Press. 2016 : 232, 633.
18 Bae HK, Seo BI. Inhibitory Activities of Rehmanniae Radix 30% Ethanol Extract on Acute Gastritis and Peptic Ulcers. Kor. J. Herbol. 2019 ; 34(2) : 1-14.   DOI
19 Ju MJ, Kwon JH, Kim HK. Physiological activities of mulberry leaf and fruit extracts with different extraction conditions. Korean J Food Preserv. 2009 ; 16 : 442-8.
20 Song HW, Ji KY, Kim BK, Yang WK, Han CK, Shin HJ, Park YC, Hwang JS, Kang HS, Kim SH. Respiratory Protective Effect of Salvia plebeia R. Br. Extracts against Ambient Particulate Matter-induced Airway Inflammation. Korean J. Medicinal Crop Sci. 2017 ; 25(5) : 269-81.   DOI
21 Editorial Committee of Oriental Medicine and Herbal Medicine. Herbal medicine. Seoul : Younglimsa. 2005 : 647-48.
22 Ge Y, Huang M, Yao YM. Biology of Interleukin-17 and Its Pathophysiological Significance in Sepsis. Front. Immunol. 2020 ; doi.org/10.3389/fimmu.2020.01558.   DOI
23 McCreanor J, Cullinan P, Nieuwenhuijsen MJ, Stewart-Evans J, Malliarou E, Jarup L, Harrington R, Svartengren M, Han IK, Ohman-Strickland P, Chung KF, Zhang J. Respiratory effects of exposure to diesel traffic in persons with asthma. N Engl J Med 2007 ; 357(23) : 2348-58.   DOI
24 Byun SH, Park SM, Kim SC, Cho IJ. Anti-fibrotic Effect of Mori Folium Extract in Hepatic Stellate Cells. Kor J Herbology. 2013 ; 28(4) : 49-55.   DOI
25 Hunyadi A, Veres K, Danko B, Kele Z, Weber E, Hetenyi A, Zupko I, Hsieh T. In vitro anti-diabetic activity and chemical characterization of an apolar fraction of Morus alba leaf water extract. Phytother Res. 2013 ; 27(6) : 847-51.   DOI
26 Naowaboot J, Pannangpetch P, Kukonqviriyapan V, Kongyingyoes B, Kukonqviriyapan U. Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocininduced chronic diabetic rats. Plant Foods Hum Nutr. 2009 ; 64 : 116-21.   DOI
27 Kim HK, Lee JY, Han HS, Kim YJ, Kim HJ, Kim YS, Kim HM, Ko SG, An HJ, Lee YJ, Park W. Immunomodulatory Effects of Liriope Platyphylla Water Extract on Lipopolysaccharide-Activated Mouse Macrophage. Nutrients. 2012 ; 4(12) : 1887-97.   DOI
28 Huang TJ, Tsai YC, Chiang SY, Wang GJ, Kuo YC, Chang YC, Wu YY, Wu YC. Anti-viral effect of a compound isolated from Liriope platyphylla against hepatitis B virus in vitro. Virus Res. 2014 ; 192 : 16-24.   DOI
29 Fonio, A. Uber ein neues Verfahren der Blutplattchenzahlung. Dtsch. Z. Chir. 1912 ; 117, 176.   DOI
30 Organization for Economic Cooperation and Development (OECD). The economic consequences of outdoor air pollution. 2016 [cited 2019 Nov 26]. Available from:https://www.oecd.org/environment/indicators-modelling-outlooks/Policy-Highlights-Economic-consequences-of-outdoor-air-pollution-web.pdf
31 Peters S, Reid A, Fritschi L, de Klerk N, Musk AW. Long-term effects of aluminium dust inhalation. Occupational and Environmental Medicine. 2013 ; 70 : 864-8.   DOI
32 van Manen MJG, Birring SS, Vancheri C, Cottin V, Renzoni EA, Russell AM, Wijsenbeek MS. Cough in idiopathic pulmonary fibrosis. European Respiratory Review. 2016 ; doi:10.1183/16000617.0090-2015.   DOI
33 Lukacs NW, Hogaboam CM, Kunkel SL. Chemokines and their receptors in chronic pulmonary disease. Curr Drug Targets Inflamm Allergy. 2005 ; 4(3) : 313-7.   DOI
34 WHO. WHO Expert Consultation: Available evidence for the fu-ture update of the WHO global air quality guidelines (AQGs). 2016 [cited 2019 Nov 26].Available from:http://www.euro.-who.int/en/health-topics/environment-and-health/air-quality/publications/2016/who-expert-consultation-available-evidence-for-the-future-update-of-the-who-global-air-quality-guidelines- aqgs-2016.
35 Brandt EB, Kovacic MB, Lee GB, Gibson AM, Acciani TH, Le Cras TD, Ryan PH, Budelsky AL, Hershey GKK. Diesel exhaust particle induction of IL-17A contributes to severe asthma. Journal of Allergy and Clinical Immunology. 2013 ; 132 : 1194-204.   DOI
36 Fuks K, Moebus S, Hertel S, Viehmann A, Nonnemacher M, Dragano N, Mohlenkamp S, Jakobs H, Kessler C, Erbel R, Hoffmann B. Long-term urban particulate air pollution, traffic, noise and arterial blood pressure. Environmental Health Perspectives. 2011 ; 119 : 1706-11.   DOI
37 Ghio AJ, Smith CB, Madden MC. Diesel exhaust particles and airway inflammation. Current Opinion in Pulmonary Medicine. 2012 ; 18 : 144-50.   DOI
38 Jo HJ, Park SW, Lee HI, Lee SW. Health effects caused by partic-ulate matter and guidelines for health care. Public Health Weekly Report. 2018 ; 11 : 458-62.
39 Barnes PJ. Cytokines as mediators of chronic asthma. American Journal of Respiratory and Critical Care Medicine. 1994 ; 150 : S42-9.   DOI
40 Wang G, Xu Z, Wang R, Al-Hijji M, Salit J, Strulovici-Barel Y, Tilley AE, Mezey JG, Crystal RG. Genes associated with MUC5AC expression in small airway epithelium of human smokers and nonsmokers. BMC Med Genomics. 2012 ; 5 : 21.   DOI
41 Freidwald WT, Levy RI, Fredrickson DS. Estimation of concentration of the low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972 ; 18(6) : 499-02.   DOI
42 National College of Korean Medicine Textbook Compilation Committee. Herbology. Seoul : Youglimsa. 2008 : 644-55.
43 Kim H. Effect of Maekmoondong-Tang on the immunomodulatory action. Korean Journal of Oriental Physiology & Pathology. 2003 ; 17 : 946-51.