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http://dx.doi.org/10.6116/kjh.2021.36.2.31.

Protective Effect of Spatholobi Caulis in Thioacetamide induced Acute Liver Injury of Rat  

Oh, Min Hyuck (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.36, no.2, 2021 , pp. 31-42 More about this Journal
Abstract
Objectives : This study was undertaken to investigate the hepatoprotective effect of Spatholobi Caulis water extract (SC) to thioacetamide (TAA)-induced acute liver injury (ALI) in rats. Methods : The rats were injected intraperitoneally with TAA (200 mg/kg body weight) and orally administered SC (100 or 200 mg/kg b.w.) daily for 3 days. Liver biomarkers were assessed by serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and ammonia levels. Malondialdehyde (MDA) was measured both serum and liver tissue. In addition, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, anti-oxidant, and inflammation-related proteins were investigated by western blot analysis. Histological examination further confirmed though hematoxylin and eosin stain. Results : The SC treatment reduced liver function markers like GOT and GPT and also remarkably decreased ammonia level. Moreover, the elevated MDA level in TAA-induced group was significantly reduced by SC treatment. NADPH oxidase expression associated with oxidative stress including NOX2, NOX4, and p47phox markedly inhibited by SC administration. SC treatment exerted anti-oxidant effect through the increase of anti-oxidant enzyme including superoxide dismutase (SOD), Catalase, and heme oxygenase-1 (HO-1). The protein expressions of inflammatory cytokines such as tumor necrosis factor-�� (TNF-��), IL-6, and IL-1�� induced by nuclear factor-kappa B (NF-��B) activation were modulated through blocking the phosphorylation of inhibitor of nuclear factor ��B�� (I��B)��. SC treatment also improved histological alterations. Conclusion : These findings suggested that SC administration may be a potential candidate for the prevention or treatment of ALI.
Keywords
Acute liver injury; Spatholobi Caulis water extract; Thioacetamide; Oxidative stress; Anti-oxidant; Anti-inflammation;
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1 Korean statistical Information Service. Official Website. Retrieved Nov. 16, 2020, from http://kostat.go.kr/assist/synap/preview/skin/doc.html?fn=synapview385219_1&rs=/assist/synap/preview.
2 Mansour HH, Hafez HF, Fahmy NM. Silymarin ModulatesCisplatin-Induced Oxidative Stress and Hepatotoxicity in Rats. Int J Biochem Mol Biol 2006 ; 39 (6): 656-661.
3 Jo WS, Nam BH, Oh SJ, Choi YJ, Kang EY, Hong SH, Jeong MH. Hepaticprotective effect and single-dose toxicity study of water extract of Cordyceps militaris grown upon Protaetia dreujtarsis. KOREAN J. FOOD SCI. TECHNOL. 2008 ; 40 (1): 106-110.
4 Ashkani-Esfahani S, Bagheri F, Emami Y, Esmaeilzadeh E, Azarpira N, Hassanabadi N, Keshtkar M, Farjam M, Koohi-Hosseinabadi O, Noorafshan A. Protective Effects of Co-Enzyme Q10 on Thioacetamide-Induced Acute Liver Damage and ItsCorrelation With Behavioral, Biochemical, and Pathological Factors. Iran Red Crescent Med J. 2016 ; 18(8) : e29166.   DOI
5 Kim OK. Protective Effects of Extracts of Diospyruskaki Folium against Hepatotoxicity in Carbon Tetrachloride Intoxicated Rats. J Korean Soc Food Sci Nutr. 2001 ; 30(1) : 97-101.
6 Jeong CH, Choi GN, Kim JH,, Kwak JH, Kim DO, Kim YJ, Heo HJ. Antioxidant activities from the aerial parts of platy codon grandiflorum. Food Chemistry. 2010 ; 118(2) : 278-282.   DOI
7 Pellegrini M. Baldari CT. Apoptosis and oxidative stress-related disease: the p66shc connection. Curr Mol Med. Medicine. 2009 ; 9(3) : 392-398.   DOI
8 Byers T, Perry G. Dietary carotenes, vitamin C, andvitamin E as protective antioxidants in human cancers. Annu. Rev. Nutr. 1992 ; 12 : 135-159.
9 Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 1976 ; 71(4) : 952-958.   DOI
10 Lu SC, Mato JM. S-Adenosylmethionine in cell growth, apoptosisand liver cancer. J Gastroenterol Hepatol. 2008 ; 23 (1) : s73-s77.   DOI
11 Lee YG, Kim SY, Kim HW. A literature study on dermatological efficacy and drug induced liver injury of Dictamnus dasycarpus Turcz. Kor. J. Herbology. 2018 ; 33 (1) : 9-15.
12 Youn DH, Kim WI, Na CS. Effects of combination pear extract with Daekumeumjagami medication on hepaticinjury induced by alcohol in mice. Kor. J. Herbology. 2015 ; 30 (1) : 51-57.   DOI
13 Colleges of Oriental Medicine. Herbologyprofessor's association. Herbology. Seoul : Young-Lim Publisher. 1992 : 445-446.
14 Brusilow SW. Hyperammonemic encephalopathy. Medicine (Baltimore). 2002 ; 81 : 240-249.   DOI
15 Fitzhugh OG, Nelson AA. Liver tumors in rats fed thiourea orthioacetamide. Science. 1948 ; 108(2014) : 626-628.   DOI
16 Hunter AL, Hosche MA, Neal RA. Thioacetamide-induced hepatic necrosis. Involvement of the mixed-function oxidase enzyme system. J Pharmacol Exp Ther. 1977 ; 200(2) : 439-448.
17 Satyabhama S, Padmanaban G. Effects of thioacetamide on cytochrome P450 synthesis in rat liver. Biochem J. 1984 ; 218(2) : 371-377.   DOI
18 KIM JH. Protective effect of purple sweet potato hot water extract added to Bacillus subtilis fermented soymilk against oxidative stress and cognition ability. Pusan National University Graduate School. Pusan. 2011.
19 Kim YY. Suppression of Reactive Oxygen Species by Inhibiting NADPH Oxidase Activity. University of Seoul Graduate School. Seoul. 2012.
20 Cho YS, Choi JH, Zhang SY, Lee JH, Hwang KK, Kim HS, Oh BH, Lee MM, Park YB, Choi YS. Relationship of Polymorphisms in the Oxidative Stress Related Genes - Paraoxonase and p22phox - to Variant Angina and Coronary Artery Stenosis in Korean. Korean Circulation J. 2003 ; 33(2) : 104-112.   DOI
21 K. A. Radermacher, K. Wingler, P. Kleikers, S. Altenhofer, J. J. Hermans, C. Kleinschnitz, S. H. Hhw, The 1027th target candidate in stroke: Will NADPH oxidase hold up?. Exp Transl Stroke Med. 2012 ; 4(1) : 11.   DOI
22 Kim JJ, Kim BW, Woo HZ, Kim DH, Cho SH. Eastern liver internal medicine. Seoul :Jipmoondang Press. 1986 : 27-36.
23 Kim JH, An CW, Kim YJ, Noh YJ, Kim SJ, HwangIH, Jeon H, Cha DS, Shin TY, Kim DK. Anti oxidative Effect of Epimedii Herba in Caenorhabditiselegans. Kor. J. Pharmacogn. 2017 ; 48(4) : 298-303.
24 Choi SI, Park SJ, Byun SH, Lee JR, Park MK, Kim SC. Effects of Spatholobi Caulis MeOH Extracton the Production of NO and Pro-inflammatory Cytokines in LPS-activated Raw 264.7 Cells. Kor. J. Herbology. 2009 ; 24(2) : 21-27.
25 Hu-Zhan Zheng(鄭虎占). Modern study of traditional chinese medicine(中藥現代硏究與應用). Beijing : xue-yuan Publisher(學苑出版社). 1998 : 2539-2546.
26 Yoon-JS. Flavonoids of spatholobus suberectus [master's thesis]. Seoul : Graduate school, Seoul University : 2003.
27 Zhu You Ping. Chinese materia medica. Harwoodacademic publisher. The Netherlands. 1998 : 468-470.
28 Cha BC, Lee EH, Noh MA. Antioxidant Activity of Spatholobus suberectus Dunn. Kor. J. Pharmacogn. 2005 ; 36(1) : 50-55.
29 Sim GS, Kim JH, Lee DH, Park SM, Pyo HB, Zhang YH, et al. Effects of the Spatholobi calulis extract on Anti oxidation and Inhibition of Matrix Metallo proteinase in Human Skin Fibroblasts. KSBB Journal. 2005 ; 20(1) : 40-45.
30 Lam TL, Lam ML, Au TK, Ip DT, Ng TB, Fong WP, Wan DC. A comparison of human imminodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs, Life Sci. 2000 ; 67(23) : 2889-2896.   DOI
31 Choi JS, Song TW, Kim DH. A Study on the effect Spatholobus suberectus Dunn on the inhibition of arthritis by collagen on the mouse. Kor. J. Herbology. 2003 ; 18 : 79-88.
32 So HG, Oh MS, Kim DH. Immunity Responses of the Spatholobus suberectus Dunn to Synovial Cells Isolated from Patients with Rheumatoid Arthritis, Korean J. Oriental Physiology & Patholog. 2003 ; 17 : 780-786.
33 Re R. Pellegrini N, Proteggente A, Pannala A,Yang M, Rice-Evans C. Antioxidant activity applyingan improved ABTS radical cation decolorizationassay. Free Radic. Biol. Med. 1999 ; 26(9-10) : 1231-1237.   DOI
34 National Law Information Center. Official Website. Retrieved Mar. 11, 2021, from https://www.law.go.kr/%ED%96%89%EC%A0%95%EA%B7%9C%EC%B9%99/%EB%8C%80%ED%95%9C%EB%AF%BC%EA%B5%AD%EC%95%BD%EC%A0%84%EC%99%B8%ED%95%9C%EC%95%BD(%EC%83%9D%EC%95%BD)%EA%B7%9C%EA%B2%A9%EC%A7%91.
35 Geng CA, Yang TH, Huang XY, Ma YB, Zhang XM, Chen JJ. Antidepressant potential of Uncaria rhynchophylla and its active flavanol, catechin, targeting melatonin receptors. J Ethnopharmacol. 2019 ; 232 : 39-46.   DOI
36 Blosis MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;26: 1199-200.   DOI
37 Folin O, Denis W. On phosphotungastic phosphomolybdic compounds as color reagent. J Biol Chem. 1912 ; 12 : 239-243.   DOI
38 Lister CE, Lancaster JE, Sutton KH, Walker JR. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. J. Sci. Food Agric. 1994 ; 64 (2) : 155-161.   DOI
39 Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978 ; 86(1) : 271-278.   DOI
40 Go MS. Clinical Application of Biochemical Liver Function Test. Dongguk Journal of Medicine. 2008 ; 15(2) :48-59.
41 Asagiri M, Takayanagi H. The molecular understanding of osteoclast differentiation. Bone. 2007 ; 40(2) : 251-264.   DOI
42 McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969 ; 244 : 6049-6055   DOI
43 Zhang D, Yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, Okada S. Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Free Radic Biol Med. 1996 ; 20 : 145-150.   DOI
44 Park SH, Lee HJ, Ryu JH, Lee SY, Shin HD, Hong JH, Seok JH, Lee CJ. Effects of Silibinin and Resveratrol on Degradation of IκB and Translocation of NF-κB p65 Induced by Tumor Necrosis Factor-α in Cultured Airway Epithelial Cells. J Pharm. 2014 ; 58(1) : 1-6.