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http://dx.doi.org/10.6116/kjh.2021.36.1.67.

Development of tablets and evaluation of ingredient content and pharmacological effects of Yukgunja-tang  

Kim, Myoung-Jin (National Institute for Korean Medicine Development)
Choi, Hye-min (National Institute for Korean Medicine Development)
Yu, Byung-Woo (National Institute for Korean Medicine Development)
Hong, Young-Ju (National Institute for Korean Medicine Development)
Ra, Chae-Suk (National Institute for Korean Medicine Development)
Kim, Min-Ju (National Institute for Korean Medicine Development)
Kim, Jung-Ok (National Institute for Korean Medicine Development)
Publication Information
The Korea Journal of Herbology / v.36, no.1, 2021 , pp. 67-76 More about this Journal
Abstract
Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development. Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 mg/kg) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment. Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured. Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines. Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.
Keywords
Yukgunja-tang tablet (YGJT-T); lipopolysaccharide (LPS); pro-inflammatory cytokines; acute gastritis; anti-inflammatory; anti-gastritis;
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