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http://dx.doi.org/10.6116/kjh.2021.36.1.1.

Protective effect of Citri Unshius Pericarpium against cadmium-induced liver damage in mice  

Noh, Gyu Pyo (College of Korean Medicine, Daegu Haany University)
Lee, Jong Rok (Department of Pharmaceutical Engineering, Daegu Haany University)
Kim, Jae Kwang (Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM))
Park, Sang Mi (College of Korean Medicine, Daegu Haany University)
Park, Sook Jahr (Department of Pharmaceutical Engineering, Daegu Haany University)
Kim, Sang Chan (College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.36, no.1, 2021 , pp. 1-8 More about this Journal
Abstract
Objective : Citri Unshius Pericarpium (Citrus unshiu peel) has been used in Korean medicine to treat indigestion, vomiting, coughing and phlegm. This study investigated the hepatoprotective effect of ethanol extract of Citrus unshiu peel (CEE) in cadmium (CdCl2)-treated mouse model. Methods : CEE was dissolved in water and administered orally to mice once a day for 7 consecutive days. The mice were then exposed to a single intraperitoneal (i.p.) injection of cadmium (4 mg/kg body weight) to induce acute hepatotoxicity. At the end of the experiment, blood and liver tissue samples were collected, analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), and histopathological evaluation. Liver damage was assessed as the percentage of degenerative areas of the hepatic parenchyma, the number of degenerative hepatocytes, and the number of infiltrated inflammatory cells. Results : In cadmium-treated rats, pretreatment with CEE significantly reduced the serum ALT and AST levels associated with liver damage. Histopathologically, CEE prevented degenerative changes on the hepatic tissues including confluent necrosis, congestions and infiltration of inflammatory cells. CEE also reduced the elevation of oxidative stress markers (nitrotyrosine and 4-hydroxynonenal) and apoptosis markers (cleaved caspase-3 and cleaved PARP) positive cells. PARP protein expression in liver tissue was also restored by CEE. Conclusion : This study showed that CEE exerted antioxidant and anti-apoptotic effects against cadmium-induced liver injury. Thus, it can be concluded that CEE can be used to prevent liver damage caused by cadmium.
Keywords
Citri Unshius Pericarpium; cadmium; nitrotyrosine; 4-hydroxynonenal; caspase-3; PARP;
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