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http://dx.doi.org/10.6116/kjh.2020.35.6.21.

Improvement Effect of Sprout of Coix lacryma-jobi var. mayuen Stapf Water Extract on DSS-Induced Ulcerative Colitis in Mice.  

Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Soon-Ae (Department of Herbal Food Cuisine & Nutrition, Daegu Haany University)
Park, Hae-Jin (DHU Bio Convergence Testing Center)
Lee, Jeong Hoon (Department of Herbal Crop Reasearch, NIHHS, RDA)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.35, no.6, 2020 , pp. 21-28 More about this Journal
Abstract
Objectives : The objective of this study was to investigate the improvement effect of Sprout of Coix lacryma-jobi var. mayuen Stapf water extract (SC) on the dextran sulfate sodium (DSS)-induced ulcerative colitis mice. Methods : The antioxidant activity of SC was measured through total polyphenol and total flavonoid content in vitro. The experiment was conducted with seven-week-old male Balb/c mice. After 1 week adaptation, acute colitis was induced by oral administration of 5% DSS dissolved in drinking water, for 7 days. And normal mice received drinking water without DSS throughout the entire experimental period. For each experiment, the mice were divided into 4 groups and 24 colitis mice were arbitrarily allocated into 3 groups (n = 8/group); Normal group, Control group, SC 100 mg/kg treated group (SCL), SC 200 mg/kg treated group (SCH). Serum and colon tissues were collected after one weeks of drug administration. Results : ROS levels, ONOO- levels, AST, and ALT in serum were decreased in SC treated groups compared to the control group. Western blotting measurements of Nrf2, HO-1, SOD, catalase, GPx-1/2, IL-4, IL-10, and Bcl2 showed that the SC treated groups was increased compared to the Control group. Also, western blot measurements of NF-κBp65, p-IκBα, COX-2, iNOS, TNF-α, IL-1β, Bax, and Caspase-3 showed that the SC treated groups was reduced compared to the Control group. Conclusion : Taken together, these results suggest that SC treatment can attenuate the DSS-induced colitis though inhibiting NF-κB pathway and enhancing Nrf2 pathway. Therefore, SC was the potential to be used as a natural therapeutic drug.
Keywords
Sprout of Coix lacryma-jobi var. mayuen Stapf; Colitis; Dextran sulfate sodium (DSS); Anti-oxidant; Inflammation; Anti-inflammation; Necrosis;
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Times Cited By KSCI : 8  (Citation Analysis)
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