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http://dx.doi.org/10.6116/kjh.2020.35.6.11.

The Comparative Study of Immunomodulatory Effect by Glycyrrhiza New Varieties and Official Compendia  

Kang, Yun-Mi (Department of Pharmacology, College of Korean Medicine, Sangji University)
Kim, Wonnam (Division of Pharmacology, College of Korean Medicine, Semyung University)
Jin, Jong-Sik (Department of Oriental Medicine Resources, Jeonbuk National University)
Lee, Jong-Hyun (Department of Pharmacy, College of Pharmacy, Dongduk Women's University)
Chang, Jae Ki (Herbal Crop Research Division, NIHHS, RDA)
Lee, Jeonghoon (Herbal Crop Research Division, NIHHS, RDA)
An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
Publication Information
The Korea Journal of Herbology / v.35, no.6, 2020 , pp. 11-19 More about this Journal
Abstract
Objective : The genus Glycyrrhiza has been used in food and traditional herbal medicine. Glycyrrhiza new varieties Wongam and Sinwongam have been developed by Korea Rural Development Administration and investigated to register on Korean Pharmacopoeia of the Ministry of Food and Drug Safety. The aim of this study is to investigate the immunomodulatory effect of Wongam and Sinwongam comparing with listed Glycyrrhiza species (Glycyrrhiza uralensis Fischer and G. glabra Linne) for evaluations about pharmacological effect of Glycyrrhiza new varieties. Methods : We studied the immunomodulatory effect of Wongam and Sinwongam compared with G. uralensis and G. glabra using THP-1 cell in vitro model. The cells were treated with phorbol 12-myristate 13-acetate (PMA) for differentiation and stimulated with lipopolysaccharides (LPS) to induce immune activation. We analyzed and compared the effects Glycyrrhiza new varieties and listed Glycyrrhiza species using nitric oxide (NO) assay, western blot, and reverse transcription-quantitative polymerase chain reaction analysis. 1) Results : Wongam and Sinwongam showed no cytotoxicity in THP-1 cells. Wongam and Sinwongam, and listed Glycyrrhiza species increased NO production, and cyclooxygenase (COX)-2 expression with or without LPS in differentiated THP-1 macrophages. Furthermore, Wongam and Sinwongam and listed Glycyrrhiza species upregulated the mRNA expressions of T helper type 1 (Th 1)-associated cytokines in LPS-stimulated THP-1 macrophages. Conclusion : These results indicated that Wongam and Sinwongam would have effect of enhancing immune response through the increase of NO and COX-2 expression, and activate Th1-associated cytokines. The findings of this study suggest the wide applicability of Glycyrrhiza new varieties.
Keywords
Immunomodulatory effect; Glycyrrhiza new varieties; Wongam; Sinwongam; immune response;
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1 Gandhi, J., L. Khera, N. Gaur, C. Paul, and R. Kaul, Role of Modulator of Inflammation Cyclooxygenase-2 in Gammaherpesvirus Mediated Tumorigenesis. Front Microbiol. 2017;8:538.
2 Kim, A.J., Y.O. Kim, J.S. Shim, and J.K. Hwang, Immunostimulating activity of crude polysaccharide extract isolated from Curcuma xanthorrhiza Roxb. Biosci Biotechnol Biochem. 2007;71:1428-38.   DOI
3 Leung, S., X. Liu, L. Fang, X. Chen, T. Guo, and J. Zhang, The cytokine milieu in the interplay of pathogenic Th1/Th17 cells and regulatory T cells in autoimmune disease. Cell Mol Immunol. 2010;7: 182-9.   DOI
4 Holley, M.M. and T. Kielian, Th1 and Th17 cells regulate innate immune responses and bacterial clearance during central nervous system infection. J Immunol. 2012;188:1360-70.   DOI
5 Steen, E.H., X. Wang, S. Balaji, M.J. Butte, P.L. Bollyky, and S.G. Keswani, The Role of the Anti-Inflammatory Cytokine Interleukin-10 in Tissue Fibrosis. Adv Wound Care (New Rochelle). 2020;9:184-198.   DOI
6 Calder, P.C. and S. Kew, The immune system: a target for functional foods? Br J Nutr. 2002;88 Suppl 2:S165-77.   DOI
7 Juan-Manuel Anaya, Yehuda Shoenfeld, Adriana Rojas-Villarraga, Roger A. Levy, Ricard Cervera, editors. Autoimmunity: From Bench to Bedside, First edition. Bogota (Colombia): El Rosario University Press; 2013. Available from:URL:https://pubmed.ncbi.nlm.nih.gov/29087650.
8 Arango Duque, G. and A. Descoteaux, Macrophage cytokines: involvement in immunity and infectious diseases. Front Immunol. 2014;5:491.
9 Locati, M., A. Mantovani, and A. Sica, Macrophage activation and polarization as an adaptive component of innate immunity. Adv Immunol. 2013;120:163-84.   DOI
10 Elhelu, M.A., The role of macrophages in immunology. J Natl Med Assoc. 1983;75:314-7.
11 Sedighinia, F., A. Safipour Afshar, S. Soleimanpour, R. Zarif, J. Asili, and K. Ghazvini, Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna J Phytomed. 2012;2: 118-24.
12 Pereira, A.C.A., R.J. Silva, P.S. Franco, A. de Oliveira Gomes, G. Souza, I.C.B. Milian, M. Ribeiro, A.M. Rosini, P.M. Guirelli, E.L.P. Ramos, T.W.P. Mineo, J.R. Mineo, N.M. Silva, E.A.V. Ferro, and B.F. Barbosa, Cyclooxygenase (COX)-2 Inhibitors Reduce Toxoplasma gondii Infection and Upregulate the Pro-inflammatory Immune Response in Calomys callosus Rodents and Human Monocyte Cell Line. Front Microbiol. 2019;10:225.   DOI
13 Leopold Wager, C.M. and F.L. Wormley, Jr., Classical versus alternative macrophage activation: the Ying and the Yang in host defense against pulmonary fungal infections. Mucosal Immunol. 2014;7:1023-35.   DOI
14 Fiore, C., M. Eisenhut, E. Ragazzi, G. Zanchin, and D. Armanini, A history of the therapeutic use of liquorice in Europe. J Ethnopharmacol. 2005;99:317-24.   DOI
15 Fiore, C., M. Eisenhut, R. Krausse, E. Ragazzi, D. Pellati, D. Armanini, and J. Bielenberg, Antiviral effects of Glycyrrhiza species. Phytother Res. 2008;22:141-8.   DOI
16 Furuhashi, I., S. Iwata, S. Shibata, T. Sato, and H. Inoue, Inhibition by licochalcone A, a novel flavonoid isolated from liquorice root, of IL-1beta-induced PGE2 production in human skin fibroblasts. J Pharm Pharmacol. 2005;57:1661-6.   DOI
17 Pastorino, G., L. Cornara, S. Soares, F. Rodrigues, and M. Oliveira, Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytother Res. 2018;32:2323-2339.   DOI
18 Dong, S., A. Inoue, Y. Zhu, M. Tanji, and R. Kiyama, Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root. Food Chem Toxicol. 2007;45:2470-8.   DOI
19 Lateef, M., L. Iqbal, N. Fatima, K. Siddiqui, N. Afza, M. Zia-ul-Haq, and M. Ahmad, Evaluation of antioxidant and urease inhibition activities of roots of Glycyrrhiza glabra. Pak J Pharm Sci. 2012;25:99-102.
20 Wang, W., X. Hu, Z. Zhao, P. Liu, Y. Hu, J. Zhou, D. Zhou, Z. Wang, D. Guo, and H. Guo, Antidepressant-like effects of liquiritin and isoliquiritin from Glycyrrhiza uralensis in the forced swimming test and tail suspension test in mice. Prog Neuropsychopharmacol Biol Psychiatry. 2008; 32:1179-84.   DOI
21 Kwon, Y.J., D.H. Son, T.H. Chung, and Y.J. Lee, A Review of the Pharmacological Efficacy and Safety of Licorice Root from Corroborative Clinical Trial Findings. J Med Food. 2020;23:12-20.   DOI
22 Dziewulska, D., T. Stenzel, M. Smialek, B. Tykalowski, and A. Koncicki, The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1. BMC Vet Res. 2018;14:148.   DOI
23 Guo, A., D. He, H.B. Xu, C.A. Geng, and J. Zhao, Promotion of regulatory T cell induction by immunomodulatory herbal medicine licorice and its two constituents. Sci Rep. 2015;5:14046.   DOI
24 Aly, A.M., L. Al-Alousi, and H.A. Salem, Licorice: a possible anti-inflammatory and anti-ulcer drug. AAPS PharmSciTech. 2005;6:E74-82.   DOI
25 Ayeka, P.A., Y. Bian, P.G. Mwitari, X. Chu, Y. Zhang, R. Uzayisenga, and E.O. Otachi, Immunomodulatory and anticancer potential of Gan cao (Glycyrrhiza uralensis Fisch.) polysaccharides by CT-26 colon carcinoma cell growth inhibition and cytokine IL-7 upregulation in vitro. BMC Complement Altern Med. 2016;16:206.   DOI
26 Lee, J.Y., J.H. Lee, J.H. Park, S.Y. Kim, J.Y. Choi, S.H. Lee, Y.S. Kim, S.S. Kang, E.C. Jang, and Y. Han, Liquiritigenin, a licorice flavonoid, helps mice resist disseminated candidiasis due to Candida albicans by Th1 immune response, whereas liquiritin, its glycoside form, does not. Int Immunopharmacol. 2009;9:632-8.   DOI
27 Y.M. Kang, W.K., J.S. Jin, J.H. Lee, J.K. Chang, J. Lee, H.J. An, The Comparative Study of Antiallergic Effect by Glycyrrhiza New Varieties and Official Compendia. Kor. J. Herbol. 2020;35:13-21.   DOI
28 Jacobs, F., D. Chaussabel, C. Truyens, V. Leclerq, Y. Carlier, M. Goldman, and B. Vray, IL-10 upregulates nitric oxide (NO) synthesis by lipopolysaccharide (LPS)-activated macrophages: improved control of Trypanosoma cruzi infection. Clin Exp Immunol. 1998;113:59-64.   DOI
29 Brubaker, J.O., C.M. Thompson, L.A. Morrison, D.M. Knipe, G.R. Siber, and R.W. Finberg, Th1-associated immune responses to beta-galactosidase expressed by a replication-defective herpes simplex virus. J Immunol. 1996;157:1598-604.
30 Huang, F.P., W. Niedbala, X.Q. Wei, D. Xu, G.J. Feng, J.H. Robinson, C. Lam, and F.Y. Liew, Nitric oxide regulates Th1 cell development through the inhibition of IL-12 synthesis by macrophages. Eur J Immunol. 1998;28:4062-70.   DOI
31 Hosseinzadeh, H. and M. Nassiri-Asl, Pharmacological Effects of Glycyrrhiza spp. and Its Bioactive Constituents: Update and Review. Phytother Res. 2015;29:1868-86.   DOI
32 Bordbar, N., M.H. Karimi, and Z. Amirghofran, The effect of glycyrrhizin on maturation and T cell stimulating activity of dendritic cells. Cell Immunol. 2012;280:44-9.   DOI
33 Peng, L.N., L. Li, Y.F. Qiu, J.H. Miao, X.Q. Gao, Y. Zhou, Z.X. Shi, Y.L. Xu, D.H. Shao, J.C. Wei, and Z.Y. Ma, Glycyrrhetinic acid extracted from Glycyrrhiza uralensis Fisch. induces the expression of Toll-like receptor 4 in Ana-1 murine macrophages. J Asian Nat Prod Res. 2011;13:942-50.   DOI
34 Kang, Y.J., B.A. Wingerd, T. Arakawa, and W.L. Smith, Cyclooxygenase-2 gene transcription in a macrophage model of inflammation. J Immunol. 2006;177:8111-22.   DOI
35 Britt, R.D., Jr., M.L. Locy, T.E. Tipple, L.D. Nelin, and L.K. Rogers, Lipopolysaccharide-induced cyclooxygenase-2 expression in mouse transformed Clara cells. Cell Physiol Biochem. 2012;29:213-22.   DOI