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http://dx.doi.org/10.6116/kjh.2020.35.3.1.

The effects of a Mixture of Puerariae Flos Extract and Citri Unshius Pericarpium Extract on Estrogenic Activities and Osteoclastogenesis  

Cho, Hosong (LG Household & Healthcare Research Park)
Lee, Boyoung (LG Household & Healthcare Research Park)
Lee, Won Kyung (LG Household & Healthcare Research Park)
Lee, Jun Ho (LG Household & Healthcare Research Park)
Park, Dongjun (LG Household & Healthcare Research Park)
Choi, Changil (LG Household & Healthcare Research Park)
Jin, Mu Hyun (LG Household & Healthcare Research Park)
Roh, Seok-Seon (Department of Korean Medicine, College of Korean Medicine, Daejeon University)
Ju, Young-Sung (Department of Herbology, College of Korean Medicine, Woosuk University)
Publication Information
The Korea Journal of Herbology / v.35, no.3, 2020 , pp. 1-8 More about this Journal
Abstract
Objectives : In this study, we examined the estrogenic activities and anti-osteo clastogenesis effects of PCE17, a mixture of PE (an extract of Puerariae Flos), and CE (an extract of Citri Unshius Pericarpium). Methods : The estrogenic effect of PCE17, PE and CE were examined by ER-β/ERE reporter gene assay and proliferation assay in 293 T and MCF-7 cells. The expression of estrogen-responsive gene and protein were checked by Real Time-PCR (RT-PCR) and Western blotting in MCF-7 cells. Inhibitory effect of PCE17, PE and CE on RANKL-induced osteoclast differentiation were evaluated by TRAP staining and RT-PCR in primary osteoclast precursors from rat bone marrow cells. Results : PCE17 and PE bind to ERs (estrogen receptors) and show estrogenic activities in 293T cells. They also stimulated the proliferation of MCF-7 cells and increased the expression of ER response gene, pS2. Tectorigenin, an active ingredient of PE, shows similar estrogenic activities in MCF-7 cells. PCE17 and CE inhibited RANKL-induced osteoclastogenesis in rat primary osteoclast precursor cells and down-regulated the osteoclast-specific genes of Nfatc1, Ctsk, and Acp5. Conclusions : In conclusion, PCE17 may have therapeutic potential in cases of menopause and osteoporosis.
Keywords
PCE17; Puerariae Flos; Citri Unshius Pericarpium; Estrogenic Activities; Anti-osteoclastogenesis;
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