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http://dx.doi.org/10.6116/kjh.2019.34.6.25.

The Anatomical Identification-key of Ephedrae Herba and its adulterant  

Lee, Guemsan (Dept. of Herbology, College of Korean Medicine, Wonkwang Univ.)
Publication Information
The Korea Journal of Herbology / v.34, no.6, 2019 , pp. 25-32 More about this Journal
Abstract
Objectives : Ephedra regeliana, although similar to the other E. sinica, E. intermedia, and E. equisetina, is not authorized by The Korean Pharmacopoeia and yet has also been imported under the name of 'Ma-Hwang'. The aim of this study was to perform a comparative anatomical evaluation of Ephedrae Sinicae Herba (ESH), Ephedrae Intermedicae Herba (EIH), Ephedrae Equisetinae Herba (EEH) and Ephedrae Regelianae Herba (ERH). Methods : Permanent paraffin-embedded specimens were produced using the paraffin-tertiary butyl alcohol (TBA) method, to visualize their anatomical features via light microscopy. Results : ESH and ERH transverse sections were in the shape of a long ellipse, while those of EIH and EEH were in the shape of a circle. These anatomical characteristics substantiated the results of external morphological examination. The sections revealed that each of the four samples showed significantly different cortexes. The cortex of ESH was wider than two thirds of the section, unlike that of the other samples which was relatively narrow. The xylems of ERH, EIH, and EEH demonstrated interconnectivity with other adjacent xylems, contrary to those in ESH. Therefore, the characteristic development of the xylems over time was used to distinguish ERH from the other. Furthermore, parenchymal morphology in the central cylinder was also a good criterion to differentiate between EIH and EEH, since EIH demonstrated parenchymal cells that were shaped like a rounded-triangle, whereas EEH parenchymal cells were chrysanthemum-shaped. Conclusions : The identification-keys established in this study would facilitate the accurate identification of microscopic features of the four varieties of 'Ma-Hwang'.
Keywords
Ephedara sinica; Ephedara intermedia; Ehpedra equisetina; Ephedra regeliana; identification; microscopy;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Herbology editorial committee of Korean medicine schools. Herbology. Seoul : Yeonglimsa. 2016 : 152-4.
2 Ministry of Food and Drug Safety. The Korean Pharmacopoeia 11th edition, 2014. The KFDA Notification No. 2014-194. 2014 : 1799-1800.
3 Pharmacopoeia Commission of the People's Republic of China. Pharmacopoeia of the People's Republic of China, 2015 ed.. Beijing : China Medical Science and Technology Press. 2015 : 320-1.
4 Ministry of Health and Welfare Taiwan. Taiwan Herbal Pharmacopeia 2nd Edition English Version. Republic of China. 2016 : 234-6.
5 The Ministry of Health, Labour and Welfare. The Japanese Pharmacopoeia 17th edition. 2016 : 1916.
6 Pharmacopoeia Committee of the DPRK. The Pharmacopoeia of Democratic People's Republic of Korea 7th edition. 2011 : 346-7.
7 eFloras. Flora of China, v4. 1999 [cited 2019 Aug 1] : 97-101. Available from : URL : http://flora.huh.harvard.edu/china/PDF/PDF04/ephedra.pdf
8 Lee SH, Yoon JH, Lee GS, Ju YS. The morphological identification for three authentic species of Ephedrae Herba. Korean Herb. Med. Inf. 2016;4(3):43-9.
9 Cui JF, Zhou TH, Zhang JS, Lou ZC. Analysis of alkaloids in Chinese Ephedra species by gas chromatographic methods. Phytochem Anal. 1991;2:116-9.   DOI
10 National Institute of Food and Drug Saftey Evaluation. The Guidline on the Visual and Organoleptic Examaination of Herbal Medicine. 2012:190-1.
11 Yang SG, Choi GY. A Comparative Anatomical Study of Saururus chinensis (Lour.) Baill. and Houttuynia cordata Thunb. (Saururaceae). Kor. J. Herbol. 2018 ; 33(3) : 11-8.   DOI
12 Doh EJ, Shin SM, Lee GS. DNA barcoding analysis of Rosase Multiflorae Fructus and its adulterants. Kor. J. Herbol. 2019 ; 34(4) : 1-8.   DOI
13 Kim JH, et al . Comparative Study on Different Species of Medicinal Herbs Used as Jeonho (Qianhu) Using Morphological Appearance and Chromatographic Fingerprint. Kor. J. Herbology 2012 ; 27(3) : 15-21.   DOI
14 Kim JH, Doh EJ, Lee GS. Chemical Differentiation of Genetically Identified Atractylodes japonica , A. macrocephala , and A. chinensis Rhizomes Using High-Performance Liquid Chromatography with Chemometric Analysis. Evidence-Based Complementary and Alternative Medicine. 2018 Aug 2;2018:4860371.   DOI
15 Ju YS, et al. Gaejeong UnGok Boncho-hak Silseupseo [New Practice in Ungok Herbology]. Jeonju : HakYe-sa. 2009 : 33-53.