Browse > Article
http://dx.doi.org/10.6116/kjh.2019.34.6.139.

Effect of herbal mixture (RAA) on hair growth in C57BL/6 mice  

Kim, Yoo-Jin (Department of Herbal Pharmacology, Daegu Haany University)
Lee, Ye Ji (Department of Herbal Pharmacology, Daegu Haany University)
Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.34, no.6, 2019 , pp. 139-146 More about this Journal
Abstract
Objective : Recently, hair has become one of the important aspects of beauty. So, there are many studies about prevention and treatment of alopecia. Finasteride and minoxidil have been known to drug for alopecia treatment. However, these agents have side effects such as irritation, pruritus, and erythema when they were used for a long time. So, we assessed hair growth effect on herbal mixture (RAA) include in Rehmanniae Radix Preparata, Achyranthis Radix, and Acanthopanacis Cortex. Methods : 6 weeks aged C57BL/6 mice were divided three treatment groups. : CON group (vehicle solution), MNXD group (positive control, 3% minoxidil), RAA group (15% RAA solution). And we applied 200 ㎕ of three groups to shaved dorsal skin every day. Hair growth effects of treatment were determined through phototrichogram by folliscope and hair follicle morphometry by H&E staining. And we assessed hair growth-related gene (VEGF, IGF-1, TGF-β1) expressions by western blot and 5-α reductase II analysis of dermal (skin) or internal organ (prostate gland). Results : Hair density and hair follicle size in MNXD or RAA group was enhanced compared to those of CON group, respectively. Also, the protein expression levels in dermal of VEGF, IGF-1 increased but TGF-β1 decreased in RAA and MNXD group, compared to CON group, respectively. 5-α reductase II levels of tissues in MNXD or RAA group significantly decreased compared to those of CON group, respectively. Conclusion : These results suggest that RAA has the promoting effect on hair growth.
Keywords
Herbal mixture; Hair growth; Alopecia; Minoxidil; C57BL/6 mice;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Hageman T, Schlutter-Bhmer B, Allam JP, Bieber T, Novak N. Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution. Contact Dermatitis. 2005 ; 53 : 85-97.
2 Botchkarev BO, Kishimoto J. Molecular control of epithelial mesenchymal interactions during hair follicle cycling. J Invest Dermatol Symp Proc. 2003 ; 8 : 46-53.   DOI
3 Cotsarelis G, Millar SE. Towards a molecular understanding of hair loss and its treatment. Trends Mol Med. 2001 ; 7 : 293-301.   DOI
4 Seven MR, Bori H, Carina VDV, Stefan E, Kerstin F, Ian AM, Kurt SS, Ralf P. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol. 2001 ; 117 : 3-15.   DOI
5 Kim MJ, Park KH, Lee IC, Kim BH. Antioxidant ability and hair growth effect of Samhwang-Sasimtang in C57BL/6 Model. Korean J Oriental Physiology & Pathology. 2014 ; 28(2) : 154-61.
6 Tsuboi R. Growth factors and hair growth. J Invest Dermatol. 1997 ; 4 : 103-8.   DOI
7 Choe BY. Search for natural products that affect hair growth. KNU Dr. thesis. 2008.
8 Lee NJ, Cho HB. The latest patent trends in Korea with related to the health of hair. Kor J Aesthet Cosmetol. 2014 ; 12(1) : 133-8.
9 Kim KO, Ku CS, Kim MJ, Park YJ, Ryu HW, Song HH, Kim JH, Oh SR. Phytoecdysones from the roots of Achyranthes japonica Nakai and their anti-atopy activity. J Appl Biol Chem. 2015 ; 58 : 13-9.   DOI
10 Mackay A, Isles C, Henderson I, Fife R, Kennedy AC. Minoxidil in the management of intractable hypertension. Quarterly J of Medicine. 1981 ; 50 : 175-90.
11 Headington JT, Novak E. Histological findings in androgenic alopecia treated with topical minoxidil. Br J Dermatol, 1982 ; 107(22) : 20-1.
12 Lee HH, Jung MY, Kim SJ. Scalp & Medical treatment. Hyunmoon Co. Seoul, Korea. 2015 ; 125-35.
13 Lee JH. Effect of Cynanchi wilfordii Radix, LyciFructus, Hovenia dulcis Extracts on hair growth in C57BL/6 mice. Kyung Hee University Master's degree. 2014.
14 Do EJ, Hwang MY, Kim SY, Lee JS, Yang DS, Yang CH, Kim MR. The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice. Kor J Herbology. 2011 ; 26(4) : 9-14.   DOI
15 Olsen EA. Female pattern hair loss. J Am Acad Dermato. 2001 ; 45(3) : 70-80.
16 Park WS, Lee CH, Lee BG, Chang IS. The extract of Thujae occidentalis semen inhibited 5${\alpha}$-reductase and androchronogenetic alopecia of B6CBAF1/j hybrid mouse. J Dermatol Sci. 2003 ; 31(2) : 91.   DOI
17 Park JS, Yeom MH, Park WS, Joo KM, Rho HS, Kim DH, Chang IS. Enzymatic hydrolysis of green tea seed extract and its activity on 5${\alpha}$-reductase inhibition. Biosci Biotechnol Biochem. 2006 ; 70(2) : 387.   DOI
18 Randall VA. Androgens and human hair growth. Clinical Endocrinology. 1994 ; 40(4) : 439-57.   DOI
19 Liu J, Kurashiki K, Shimizu K, Kondo R. 5${\alpha}$-reductase inhibitory effect of triterpenoids isolated from Ganoderma lucidum. Biol Pharm Bull. 2006 ; 29(2) : 392.   DOI
20 Yoon BY, Park HS. A study on the factors related scalp care service utilization of women. Journal of the Korean Society of Cosmetology. 2010 ; 16 : 1-14.
21 Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH. Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 2004 ; 34 : 91.   DOI
22 Dan B, Steven C, Erich S, Andrew G. Chinese herbal medicine Materia medica 3rd edition. Gary Niemeier. USA. 2004 ; 742.
23 Professor committee of Herbal Medicine in Korea. Herbal medicine. The Korean pharmaceutical association. Seoul. 1995 ; 785-6.
24 Messenger AG. The control of hair growth and pigmentation. In : Olsen EA. ed. Disorders of hair growth : diagnosis and treatment. NY McGraw-Hill. 1994 ; 39-58.
25 Kang H, Kang TW, Lee SD, Park YM, Kim HO, Kim SY. The changing patterns of hair density and thickness in South Korean women with hair loss : clinical office-based phototrichogram analysis. Int J Dermatol. 2009 ; 48 : 14-21.   DOI
26 Dallob AL, Sadick NS, Unger W, Lipert S, Geissler LA, Gregoire SL, Nguyen HH, Moore EC, Tanaka WK. The effects of finasteride, a 5 alphareductase inhibitor, on scalp skin testosterone and dihydrotestosterone concentrations in patients with male pattern baldness. J Clin Endocrinol Metab. 1994 ; 79(3) : 703-6.   DOI
27 Hamada K, Hirotsu S, Uchiwa H, Yamazaki S, Suzuki K. Proinflammatory cytokine interleukin-1 ${\alpha}$ is downregulated during anagen phase of hair cycle in vivo. J Dermatol. 2003 ; 33(3) : 195-8.
28 Hibino T, Nishiyama T. Role of TGF-${\beta}$2 in the human hair cycle. J Dermatol. 2004 ; 35 : 9-18.
29 Hamamoto T, Mori Y. Sulfation of minoxidil in keratinocytes and hair follicles. Res Commun Chem Pathol Pharmacol. 1989 ; 66 : 33-44.
30 Chaudhary UB, Turner JS. Finasteride. Expert Opin Drug Metab Toxicol. 2010 ; 6 : 873-81.   DOI
31 Su HY, Hickford GH, Bickerstaffe R, Palmer BR. Insulin-like growth factor 1 and hair growth. Dermatol Online J. 1999 ; 5(2) : 1.
32 Lachgar S, Moukadiri H, Jonca F, Charveron M, Bouhaddioui N, Gall Y, et al. Vascular endothelial growth factor is an autocrine growth factor for hair dermal papilla cells. J Invest Dermatol. 1996 ; 106(1) : 17-23.   DOI
33 Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest. 2001 ; 107(4) : 409-17.   DOI
34 Itami S, Kurata S, Takayasu S. Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-1 dermal papilla cells. Biochem Biophys Res Commun. 1995 ; 212(3) : 977-89.