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http://dx.doi.org/10.6116/kjh.2019.34.5.29.

Comparative Study of Bang-poong (root of Saposhnikovia divaricata Schischkin) and Related Species on Neuroprotective and Acetylcholinesterase Inhibitory Effects  

Ju, In Gyoung (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University)
Lee, Seungmin (Department of Oriental pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Choi, Jin Gyu (Department of Oriental pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Oh, Myung Sook (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.34, no.5, 2019 , pp. 29-37 More about this Journal
Abstract
Objectives : Bang-poong (Saposhnikovia divaricata; SD) was traditionally used to treat inflammatory disorders. In this study, we aimed to investigate whether Bang-poong and related species including SD, Glehnia littoralis (GL), and Peucedanum japonicum (PJ) possess neuroprotective effects and acetylcholinesterase (AChE) inhibitory activities. Methods : Roots of SD, GL and PJ were extracted with distilled water (DW) or 70% ethanol (EtOH). We assessed 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities of the extracts. To examine neuroprotective effects, we measured cell viability in PC12 or HT22 cells after treatment of the extracts with $H_2O_2$ or amyloid-beta ($A{\beta}$). To assess anti-neuroinflammatory effects, we measured the nitric oxide (NO) levels after treatment with the extracts and lipopolysaccharide (LPS) in BV2 microglial cells. In addition, we performed AChE inhibition assay to explore effects of the extracts on the cholinergic system. Results : DW and EtOH extracts of SD, GL and PJ showed mild DPPH free radical scavenging activities. Also, DW extracts of GL and PJ showed protective effects against $H_2O_2$-induced toxicity in PC12 cells. In LPS-activated BV2 cells, EtOH extracts of SD, GL and PJ exerted inhibitory effects on NO production. Meanwhile, DW extracts of SD, GL and PJ inhibited the $A{\beta}$-induced cell death in HT22 cells. In addition, DW and EtOH extracts of GL exhibited remarkable inhibitory activities on AChE. Conclusions : We demonstrated that SD, GL and PJ exert anti-oxidative, anti-neuroinflammatory and AChE inhibitory activities. These results indicate that SD, GL and PJ could be potential candidates for neurological disorders.
Keywords
Saposhnikovia divaricata; Glehnia littoralis; Peucedanum japonicum; neuroprotection; neuroinflammation; acetylcholinesterase inhibition;
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1 Ferreira-Vieira TH, Guimaraes IM, Silva FR, Ribeiro FM. Alzheimer's disease: Targeting the Cholinergic System. Curr Neuropharmacol. 2016 ; 14(1) : 101-15.   DOI
2 Seo BI, Kwon DY, Choi HY, Lee JH, Oh MS, Bu YM. Medicinal Herbology. 8th rev. ed. Seoul : Younglim-Sa. 2012 : 144-5.
3 Gu YR, Kim SW, Son YW, Hong JH. Antioxidant activities of solvent extracts from different Glehnia Radix parts and their inhibitory effect against nitric oxide production in Raw 264.7 cell. Korean journal of food preservation. 2017 ; 24(1) : 116-24.   DOI
4 Kang SY, Oh TW, Kim JW, Park YK. Effect of the water extract of Peucedani Japonici Radix on ovalbumin-induced allergic asthma in mice. Kor J Herbology 2013 ; 28(6) : 1-7.   DOI
5 Korea Food and Drug Administration. The Guideline on the Visual and Organoleptic Examination of Herbal Medicine. Seoul : Korea Food and Drug Administration. 2009 : 1 : 49, 103, 72.
6 Seo UM, Zhao BT, Kim YH, Kang JS, Son JK, Woo MH. Simultaneous analysis of seven marker compounds from Saposhnikoviae Radix, Glehniae Radix and Peucedani Japonici Radix by HPLC/PDA. Arch Pharm Res. 2016 ; 39(5) : 695-704.   DOI
7 Kreiner J, Pang E, Lenon GB, Yang AWH. Saposhnikoviae divaricata: a phytochemical, pharmacological, and pharmacokinetic review Chin J Nat Med. 2017 ; 15(4) : 255-64.
8 Yuan Z, Tezuka Y, Fan W, Kadota S, Li X. Constituents of the underground parts of Glehnia littoralis. Chem Pharm Bull. 2002 ; 50(1) ; 73-7.   DOI
9 WK Whang, SJ Lee, HH Kim, HK Cho, KS Lee, IH Kang, IH Ham. Standardization of Peucedani Radix. Kor J Pharmacogn. 2001 ; 32(4) : 292-6.
10 Tai J, Cheung S. Anti-proliferative and antioxidant activities of Saposhnikovia divaricata. Oncol Rep. 2007 ; 18(1) : 227-34.
11 Chun JM, Kim HS, Lee AY, Kim SH, Kim HK. Anti-Inflammatory and Antiosteoarthritis Effects of Saposhnikovia divaricata ethanol Extract: In Vitro and In Vivo Studies. Evid Based Complement Alternat Med. 2016 ; 2016 : 1984238.
12 Okuyama E, Hasegawa T, Matsushita T, Fujimoto H, Ishibashi M, Yamazaki M. Analgesic components of saposhnikovia root (Saposhnikovia divaricata). Chem Pharm Bull (Tokyo). 2001 ; 49(2) : 154-60.   DOI
13 Wang X, Jiang X, Yu X, Liu H, Tao Y, Jiang G, Hong M. Cimifugin suppresses allergic inflammation by reducing epithelial derived initiative key factors via regulating tight junctions. J Cell Mol Med. 2017 ; 21(11) : 2926-36.   DOI
14 Kuo YC, Lin YL, Huang CP, Shu JW, Tsai WJ. A tumor cell growth inhibitor from Saposhnikovae divaricata. Cancer Invest. 2002 ; 20(7-8) : 955-64.   DOI
15 Chang CZ, Wu SC, Kwan AL, Lin CL. 4'-O-$\beta$-D-glucosyl-5-O-methylvisamminol, an active ingredient of Saposhnikovia divaricata, attenuates high-mobility group box 1 and subarachnoid hemorrhage-induced vasospasm in a rat model. Behav Brain Funct. 2015 ; 11(1) : 28.   DOI
16 Ng TB, Liu F, Wang HX. The antioxidant effects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostellaria heterophylla and Glehnia littoralis. J Ethnopharmacol. 2004 ; 93 (2-3) : 285-8.   DOI
17 Lee JW, Lee C, Jin Q, Yeon ET, Lee D, Kim SY, Han SB, Hong JT, Lee MK, Hwang BY. Pyranocoumarins from Glehnia littoralis inhibit the LPS-induced NO production in macrophage RAW 264.7 cells. Bioorg Med Chem Lett. 2014 ; 24(12) : 2717-9.   DOI
18 McCutcheon AR, Ellis SM, Hancock RE, Towers GH. Antifungal screening of medicinal plants of British Columbian native peoples. J Ethnopharmacol. 1994 ; 44(3) : 157-69.   DOI
19 de la Cruz JF, Vergara EJ, Cho Y, Hong HO, Oyungerel B, Hwang SG. Glehnia littoralis Root Extract Induces G0/G1 Phase Cell Cycle Arrest in the MCF-7 Human Breast Cancer Cell Line. Asian Pac J Cancer Prev. 2015 ; 16(18) : 8113-7.   DOI
20 Yoon T, Lee do Y, Lee AY, Choi G, Choo BK, Kim HK. Anti-inflammatory effects of Glehnia littoralis extract in acute and chronic cutaneous inflammation. Immunopharmacol Immunotoxicol. 2010 ; 32(4) : 663-70.   DOI
21 Huang GJ, Deng JS, Liao JC, Hou WC, Wang SY, Sung PJ, Kuo YH. Inducible nitric oxide synthase and cyclooxygenase-2 participate in anti-inflammatory activity of imperatorin from Glehnia littoralis. J Agric Food Chem. 2012 ; 60(7) : 1673-81.   DOI
22 Yoon T, Cheon MS, Lee AY, Lee do Y, Moon BC, Chun JM, Choo BK, Kim HK. Anti-inflammatory activity of methylene chloride fraction from Glehnia littoralis extract via suppression of NF-kappa B and mitogen-activated protein kinase activity. J Pharmacol Sci. 2010 ; 112(1) : 46-55.   DOI
23 Matsuura H, Saxena G, Farmer SW, Hancock RE, Towers GH. Antibacterial and antifungal polyine compounds from Glehnia littoralis ssp. leiocarpa. Planta Med. 1996 ; 62(3) : 256-9.   DOI
24 Nugara RN, Inafuku M, Iwasaki H, Oku H. Partially purified Peucedanum japonicum Thunb extracts exert anti-obesity effects in vitro. Nutrition. 2014 ; 30(5) : 575-83.   DOI
25 Nukitrangsan N, Okabe T, Toda T, Inafuku M, Iwasaki H, Oku H. Effect of Peucedanum japonicum Thunb extract on high-fat diet-induced obesity and gene expression in mice. J Oleo Sci. 2012 ; 61(2) : 89-101.   DOI
26 Kim JM, Noh EM, Kim HR, Kim MS, Song HK, Lee M, Yang SH, Lee GS, Moon HC, Kwon KB, Lee YR. Suppression of TPA-induced cancer cell invasion by Peucedanum japonicum Thunb. extract through the inhibition of $PKC{\alpha}$/NF-${\kappa}B$-dependent MMP-9 expression in MCF-7 cells. Int J Mol Med. 2016 ; 37(1) : 108-14.   DOI
27 Chun JM, Lee AR, Kim HS, Lee AY, Gu GJ, Moon BC, Kwon BI. Peucedanum japonicum extract attenuates allergic airway inflammation by inhibiting Th2 cell activation and production of pro-inflammatory mediators. J Ethnopharmacol. 2018 ; 211 : 78-88.   DOI
28 Kim JM, Erkhembaatar M, Lee GS, Lee JH, Noh EM, Lee M, Song HK, Lee CH, Kwon KB, Kim MS, Lee YR. Peucedanum japonicum Thunb. ethanol extract suppresses RANKL-mediated osteoclastogenesis. Exp Ther Med. 2017 ; 14(1) : 410-6.   DOI
29 Chun JM, Lee AY, Kim JS, Choi G, Kim SH. Protective Effects of Peucedanum japonicum Extract against Osteoarthritis in an Animal Model Using a Combined Systems Approach for Compound-Target Prediction. Nutrients. 2018 ; 10(6) : E754.   DOI
30 Takeuchi N, Kasama T, Aida Y, Oki J, Maruyama I, Watanabe K, Tobinaga S. Pharmacological activities of the prenylcoumarins, developed from folk usage as a medicine of Peucedanum japonicum THUNB. Chem Pharm Bull (Tokyo). 1991 ; 39(6) : 1415-21.   DOI
31 Chen IS, Chang CT, Sheen WS, Teng CM, Tsai IL, Duh CY, Ko FN. Coumarins and antiplatelet aggregation constituents from Formosan Peucedanum japonicum. Phytochemistry. 1996 ; 41(2) : 525-30.   DOI
32 Nugara RN, Inafuku M, Takara K, Iwasaki H, Oku H. Pteryxin: a coumarin in Peucedanum japonicum Thunb leaves exerts antiobesity activity through modulation of adipogenic gene network. Nutrition. 2014 ; 30(10) : 1177-84.   DOI
33 Park JH, Lee TK, Yan BC, Shin BN, Ahn JH, Kim IH, Cho JH, Lee JC, Hwang IK, Kim JD, Hong S, Lee YJ, Won MH, Kang IJ. Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brainderived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area. Chin Med J (Engl). 2017 ; 130(15) : 1796-803.   DOI
34 Park JH, Shin BN, Ahn JH, Cho JH, Lee TK, Lee JC, Jeon YH, Kang IJ, Yoo KY, Hwang IK, Lee CH, Noh YH, Kim SS, Won MH, Kim JD. Glehnia littoralis Extract Promotes Neurogenesis in the Hippocampal Dentate Gyrus of the Adult Mouse through Increasing Expressions of Brain-Derived Neurotrophic Factor and Tropomyosin-Related Kinase B. Chin Med J (Engl). 2018 ; 131(6) : 689-95.   DOI
35 Ju IG, Choi JG, Kim N, Kwak C, Lee JK, Oh MS. Peucedani Japonici Radix ameliorates lipopolysaccharideinduced neuroinflammation by regulating microglial responses. Neurosci Lett. 2018 ; 686 : 161-7.   DOI
36 Song HR, Lee HY, Shim SH, Kwon YJ. Neuroinflammation and Psychiatric Illness. Korean J Biol Psychiatry. 2016 ; 23(1) : 12-7.
37 Islam MT. Oxidative stress and mitochondrial dysfunction-linked neurodegenerative disorders. Neurol Res. 2008 ; 39(1) : 73-82.   DOI
38 Haam J, Yakel JL. Cholinergic modulation of the hippocampal region and memory function. J Neurochem. 2017 ; 142 Suppl 2 : 111-21.   DOI
39 Kim N, Choi JG, Park S, Lee JK, Oh MS. Butterbur Leaves Attenuate Memory Impairment and Neuronal Cell Damage in Amyloid Beta-Induced Alzheimer's Disease Models. Int J Mol Sci. 2018 ; 19(6) : 1644.   DOI
40 Ellman GL, Courtney KD, Andres Jr V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 ; 7 : 88-90.   DOI
41 Ju MS, Kim HG, Choi JG, Ryu JH, Hur J, Kim YJ, Oh MS. Cassiae semen, a seed of Cassia obtusifolia, has neuroprotective effects in Parkinson's disease models. Food Chem Toxicol. 2010 ; 48 : 2037-44.   DOI
42 Iqbal J, al-Rashida M, Babar A, Hameed A, Khan MS, Munawar MA, Khan AF. Cholinesterase Inhibitory Activities of N-Phenylthiazol-2-Amine Derivatives and their Molecular Docking Studies. Med Chem. 2015 ; 11(5) : 489-96.   DOI
43 Seo BI, Kwon DY, Choi HY, Lee JH, Oh MS, Bu YM. Medicinal Herbology. 8th rev. ed. Seoul : Younglim-Sa. 2012 : 345.
44 Lee WC, Jeon WJ, Shin GJ. An Experimental Studies on the alleviation effects of Daebangpoongtang in LPS-induced arthritis. Dongguk Journal of the Institute of Oriental Medicine. 2009 ; 9:35-49.
45 Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Science and Technology International. 2002 ; 8 : 121-37.   DOI
46 Fetler L, Amigorena S. Neuroscience. Brain under surveillance: the microglia patrol. Science. 2005 ; 309 : 392-3.   DOI
47 Catorce MN, Gevorkian G. LPS-induced Murine Neuroinflammation Model: Main Features and Suitability for Pre-clinical Assessment of Nutraceuticals. Curr Neuropharmacol. 2016 ; 14(2) : 155-64.   DOI
48 Kamino T, Shimokura T, Morita Y, Tezuka Y, Nishizawa M, Tanaka K. Comparative analysis of the constituents in Saposhnikoviae Radix and Glehniae Radix cum Rhizoma by monitoring inhibitory activity of nitric oxide production. J Nat Med. 2016 ; 70(2) : 253-9.   DOI
49 Selkoe DJ. Alzheimer's disease results from the cerebral accumulation and cytotoxicity of amyloid beta-protein. J Alzheimers Dis. 2001 ; 3 : 75-80.   DOI