Browse > Article
http://dx.doi.org/10.6116/kjh.2019.34.4.1.

DNA barcoding analysis of Rosase Multiflorae Fructus and its adulterants  

Doh, Eui jeong (Dept. of Herbology, College of Korean Medicine, Wonkwang Univ)
Shin, Sangmun (Dept. of Herbology, College of Korean Medicine, Wonkwang Univ)
Lee, Guemsan (Dept. of Herbology, College of Korean Medicine, Wonkwang Univ)
Publication Information
The Korea Journal of Herbology / v.34, no.4, 2019 , pp. 1-8 More about this Journal
Abstract
Objectives : Rosae Multiflorae Fructus is a traditional medicine derived from the fruit of Rosa multiflora Thunb. a member of the Rosaceae family. Even though it has a single origin, the possibility of adulterants has always existed. In fact, we had discovered suspicious commercial samples of Rosae Multiflorae Fructus, imported from China. Methods : To define the taxonomic origin of Rosae Multiflorae Fructus and its adulterants, DNA barcode analysis of the internal transcribed spacer, trnL-F intergenic spacer, and psbA-trnH sequences was carried out. These DNA barcode sequences from the correct origin of Rosae Multiflorae Fructus were analyzed and compared with those of other samples from genus Rosa used as medicinal herbs. Results : The analyses of the three DNA barcode sequences efficiently distinguished Rosae Multiflorae Fructus from six other species in genus Rosa and also separated each species used in this study. According to the DNA barcoding results, none of the suspicious commercial samples were Rosae Multiflorae Fructus. RMF09 was identified as Rosa acicularis, whereas RMF10 and RMF11 were identified as Rosa davurica and Rosa rugosa, respectively. These results corroborated the existence of adulterants of Rosae Multiflorae Fructus. Conclusions : Our research provides useful information that could be used as a criterion for distinguishing between Rosae Multiflorae Fructus and its adulterants. These results will help in the prevention of adulteration and also suggest effective methods for verifying the origin of commercial herbal medicines derived from genus Rosa.
Keywords
authentication; DNA barcoding; Rosase Multiflorae Fructus; Rosaceae; ITS;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Heo J (許浚, 1546-1615). Principles and Practice of Eastern Medicine [東醫寶鑑]. New Translation of Dongui Bogam. Seoul : Bubin Publishers Co.. 2012 : 2013.
2 The Korean Herbal Pharmacopoeia 4th edition, 2012. 1st supplement, 2013. Partial amendment. 2016, 2017 : 284-5.
3 The Japanese Pharmacopoeia 17th edition. 2016 : 1742.
4 The Pharmacopoeia of Democratic People's Republic of Korea 7th edition. 2011 : 527-8.
5 State Administration of Traditional Chinese Medicine of the People's Republic of China. Chinese Materia Medica [中華本草], vol 4. Shanghai : Shanghai Science and Technology Publishing Company. 1999 : 231-2.
6 Tao HJ (陶弘景, 456-536). Bencaojing Jizhu [本草經 集注]. Chang ZJ, Chang YS, eds. Bencaojing Jizhu (jijiao ben). Beijing : People's Medical Publishing House. 1994 : 309-10.
7 Lee GS. The anatomical identification-key of Dipasci Asperi Radix, Phlomoidis Radix and Dipsaci Japonici Radix. Kor. J. Herbol. 2018 ; 33(4) : 27-33.   DOI
8 Moon BC, Kim WJ, Yang SG, Park IK, Yeo SM, Noh PR. Molecular authentication of Lepidii seu Descurainiae Semon by the development of matK amplification primers and analysis of sequence. Kor. J. Herbol. 2018 ; 33(3) : 25-35 .   DOI
9 Kim JH, Lee GS, Choi GY, Hwang SY, Kim HJ, Jeong SI, Ju YS. A study on external.internal morphology and pattern analysis of Atractylodes Rhizomes. Kor. J. Herbol. 2009 ; 24(2) : 77-85.
10 White TJ, Bruns T, Lee SJ, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc. 1990 ; 18 : 315-322.
11 Taberlet P., Gielly L., Pautou G., Bouvet J. Universal primers for amplification of three non-coding regions of the chloroplast DNA. Plant Mo. Bio. 1991 ; 17 : 1105-1109.   DOI
12 Sang T, Crawford DJ, Stuessy TF. Chloroplast DNA phylogeny reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Am. J. Bot. 1997 ; 84 : 1120- 1136. PMID:21708667   DOI
13 Yi OS, Jovel EM, Towers GHN, Wahbe TR, Cho D.W. Antioxidant and antimicrobial activities of native Rosa sp. from British Columbia, Canada. Int. J. Food Sci. Nutr.. 2007 ; 58 : 178-189.   DOI
14 Gu C, Robertson KR. Rosa L. In: Team FoCe, ed. Flora of China St. Louis, Mo : Missouri Botanical Garden Press. 2003.
15 Cutler RR. Medicinal and pharmacutical uses. Encycloopedia of rose science. In : Roberts A, Denbener T, Gudin S, eds. Amsterdam : Elsevier. 2003. 716-726
16 Jager AK, Eldeen IMS, van Staden J. COX-1 and-2 activity of rose hip. Phytother. Res.. 2007 ; 21 : 1251-1252.   DOI
17 Guimaraes R, Barros L, Carvalho AM, Ferreira ICFR. Studies on chemical constituents and activity of Rosa micrantha: an alternative antioxidant source for food, pharmaceutical, or cosmetic application. J. Agric. Food Chem. 2010 ; 58 : 6277-6284.   DOI
18 Fougere-Danezan M, Joly S, Bruneau A, Gao XF, Zhang LB. Phylogeny and biogeography of wild roses with specific attention to polyploids. Ann. Bot. 2015 ; 115 : 275-291.   DOI
19 Debener T, Bartels C, Mattiesch L. RAPD analysis of genetic variation between a group of rose cultivars and selected wild rose species. Mol. Breed. 1996 ; 2 : 321-327.   DOI
20 Milan T, Osuna F, Cobos S, Torres A, Cubero J. Using RAPDs to study phylogenetic relationships in Rosa. Theor. Appl. Genet. 1996 ; 92 : 273-277.   DOI
21 Jan CH, Byrne DH, Manhart J, Wilson H. Rose germplasm anaysis with RAPD markers. Hortscience. 1999 ; 34 : 341-345.   DOI
22 Wissemann V, Ritz CM. The genus Rosa (Rosoideae, Rosaceae) revisited: moleclar anaysis of nrITS-1 and atpB-rbcL intergenic spacer (IGS) versus conventional taxonomy. Bot. J. Linn. Soc. 2005 ; 147 : 275-290.   DOI
23 Scariot V, Akkak A, Botta R. Characterization and genetic relationships of wild species and old garden roses based on microsatellite analysis. J. Am. Soc. Hortic. Sci. 2006 ; 131 : 66-73.   DOI
24 Rehder A. Manual of cultivated trees and shrubs hardy in North America, ed. 2. New York : Macmillan. 1940.
25 Koopman WJI, Wissemann V, De Cock K. AFLP markers as a tool to reconstruct complex relationships: a case study in Rosa (Rosaceae). Am. J. Bot. 2008 ; 95 : 353-366.   DOI
26 Bruneau A, Starr JR, Joly S. Phylogenetic relationships in the genus Rosa: new evidence from chloroplast DNA sequences and an appraisal of current knowledge. Syst. Bot. 2007 ; 32 : 366-378.   DOI
27 Qiu XQ, Zhang H, Wang QG. Phylogenetic relationships of wild rose in China based on nrDNA and matK data. Sci. Hortic. 2012 ; 140 : 45-51.   DOI