Browse > Article
http://dx.doi.org/10.6116/kjh.2019.34.3.45.

The Effects of Astragali Radix on Hypothyroidism Rat Model Induced by 6-Propyl-2-thiouracil(PTU)  

Lee, Ji Hye (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Koo, Jin Suk (Department of Bioresource Science, Andong National University)
Roh, Seong Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Ji Ha (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Seo, Bu Il (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.34, no.3, 2019 , pp. 45-53 More about this Journal
Abstract
Objectives : In present study, we investigated a therapeutic effect of Astragali Radix on hypothyroidism rat model induced by 6-Propyl-2-thiouracil (PTU). Methods : Six-week-old male Sprague-Dawley rats were divided into five groups : Group one included the normal mice. Group two was administrated PTU. Group three and four were administrated the aqueous extract of Astragali Radix 150 and 300 mg/kg before start of PTU treatment. Group five (Positive control) was administrated with levothyroxine 0.5 mg/kg. During this moment the body weight, liver $H_2O_2$ and catalase (CAT) amount, serum thyroid hormone, serum asparte aminotransferase (AST) and alanine aminotransferase (ALT), gland weights were measured with histopathological changes of thyroid glands. These results were compared with levothyroxine 0.5 mg/kg treated rats. Results : The PTU treatment lead to marked decreases of body weight, level of thyroid hormone in serum and liver CAT activation. Also, PTU treatment increased thyroid gland weight, thyroid gland hormone TSH, liver $H_2O_2$ amount and level of AST in serum. On the other hands, the administration of Astragali Radix extract increased body weight gains and ameliorated histopathological changes of thyroid such as hyperplasia of follicular cells with of follicular colloid contents and sizes. In addition, the administration of Astragali Radix extract increased level of $T_4$ in serum, CAT activation in liver. Moreover, the administration of Astragali Radix extract decreased levels of TSH and AST in serum and $H_2O_2$ amount in liver Conclusions : This study suggests that Astragali Radix extract has therapeutic effects on hypothyroidism via promoting thyroid hormone production.
Keywords
Astragali Radix; Hypothyroidism; 6-Propyl-2-thiouracil; PTU;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Prieto JM, Recio MC, Giner RM, Manez S, Giner-Larza EM, Ríos JL. Influence of traditional Chinese anti-inflammatory medicinal plants on leukocyte and platelet functions. J Pharm Pharmacol. 2003 ; 55 : 1275-82.   DOI
2 Das K, Chainy GB. Modulation of rat liver mitochondrial antioxidant defence system by thyroid hormone. Biochim Biophys Acta. 2001 ; 1537 : 1-13   DOI
3 Sarandol E, Tas S, Dirican M, Serdar Z. Oxidative stress and serum paraoxonase activity in experimental hypothyroidism: effect of vitamin E supplementation. Cell Biochem Funct. 2005 ; 23 : 1-8.   DOI
4 Subudhi U, Das K, Paital B, Bhanja S, Chainy GB. Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats. Life Sci. 2009 ; 84 : 372-9.   DOI
5 O'Connor JC, Frame SR, Ladics GS. Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators. Toxicol Sci. 2002 ; 69 : 79-91.   DOI
6 Kavutcu M, Canbolat O, Ozturk S, Olcay E, Ulutepe S, Ekinci C, Gökhun IH, Durak I. Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs : effects of vitamins E and C. Nephron. 1996 ; 72 : 269-74.   DOI
7 Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ : Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 ; 193 : 265-75.   DOI
8 Pick E, Keisari Y. Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages--induction by multiple nonphagocytic stimuli. Cell Immunol. 1981 ; 59 : 301-18.   DOI
9 Roti E, Minelli R, Gardini E, Braverman LE. The use and misuse of thyroid hormone. Endocr Rev. 1993 ; 14 : 401-24.   DOI
10 Aebi H. Catalase. In : Bergmeyer HU(Ed.), Methods in Enzymatic Analysis. New York : Academic Press Inc. 1974 : 673-686.
11 Smith DW, Klein AM, Henderson JR, Myrianthopoulos NC. Congenital hypothyroidism-signs and symptoms in the newborn period. J Pediatr. 1975 ; 87 : 958-62.   DOI
12 Doo HK. Nephro-Endocrine system of Korean Medicine. Seoul : Korean Medicine Laboratory. 1993 : 729, 867-74, 1042, 1059-65.
13 Choi JY, et al. Effects of Sipjeondaebotang on the white rat Hypothyroidism Induced by PTU (6-n-propyl-2-thiouracil). Official Journal of The Korean Medicine Society For The Herbal Formula Study. 2016 ; 24 : 131-52.   DOI
14 Park EY, Kim DC. Effects of Insamyangyoung -tang Aqueous Extracts on the Hypothyroidism Induced by Propylthiouracil in Rats. J Korean Obstet Gynecol. 2015 ; 28 : 55-75   DOI
15 on JH, Kim DC. Effects of Yikgeebohyul -tang Aqueous Extracts on the Rat Hypothyroidism Induced by Propylthiouracil. J Korean Obstet Gynecol. 2015 ; 28 : 54-73.   DOI
16 Yu-Rim Song, Kyung-Mi Park, Seung-Jeong Yang, Eun-Kyu Lee, Seung-Ho Lee, Seong-Hee Cho. Effects of Yijung-tang (YJT) on Experimental Hypothyroidism in Mice. J Korean Obstet Gynecol. 2017; 30 : 1-15.   DOI
17 Kim SJ, Kim DC. Effects of Jaeumkanghwa -tang on the Rat Hypothyroidism Induced by Propylthiouracil (PTU). J Korean Obstet Gynecol. 2014 ; 27 : 41-64
18 Cho CS, Kim DB, Kim CJ. The Effects of Danggwisaeyeoktang on the Hypothyroidism of Rats. Kor. J. Herbol. 2007 ; 22 : 95-102.
19 Kim DH, Choi JS, Kim CJ, Cho CS. The Effects of Daeyoungjeon (DYJ) on the Hypothyroidism in Rats. Kor. J. Herbol. 2007 ; 22 : 35-43.
20 Choi IG, Chae EY, Chang SK, Cho CS, Kim CJ. Effects of Jinmutang (JMT) on Hypothyroidism in Rats. Korean J. Orient. Int. Med. 2006 ; 27 : 879-87.
21 Lee SJ, Back SH, Ahn SY. Lee BC, Ahn YM. Effects of Cistanche Deserticola on Thyroid Function in Hypothyroidism Rat Model Induced by PTU(6-Propyl, 2-thiouracil). Korea J. Oriental Physiology & Pathology. 2011 ; 25 : 989-95.
22 Hong MJ, Ahn SY, Lee BC, Ahn YM. The Effects of Epimedii Herba on a Hypothyroidism Rat Model induced by PTU(6-Propyl, 2-thiouracil). Journal of Pharmacopuncture. 2011 ; 14 : 13-22.   DOI
23 Lee SH, Lee BC, Ahn YM, Doo HK, Ahn SY. The Effects of Aconiti Radix on Thyroid Function in Hypothyroidism Rat Model Induced by 6-propyl-2-thiouracil (PTU). Korean J. Orient. Int. Med. 2007 ; 28 : 275-83.
24 Textbook Compilation Committee of National Universitiy of Korean Medicine. Herbology. Seoul: Yeonglimsa. 2007 : 576-8.
25 Kim MS, Yoon CY, Cho YM, Jung HS, Shin CS, Park KS, Kim SY, Cho BY, Lee HK. Changes in Plasma Leptin Levels Relating to Short-Term Thyroid Manipulation in Rats. Endocrinology and Metabolism. 2002 ; 17 : 197-205
26 Yang Y, Gordon CJ. Regulated hypothermia in the hypothyroid rat induced by administration of propylthiouracil. Am J Physiol. 1997 ; 272 : 1390-5.   DOI
27 Santi A, Duarte MM, de Menezes CC, Loro VL. Association of lipids with oxidative stress biomarkers in subclinical hypothyroidism. Int J Endocrinol. (2012) 2012:856359. doi: 10.1155/2012/856359   DOI
28 Simon-Giavarotti KA, Giavarotti L, Gomes LF, Lima AF, Veridiano AM, Garcia EA, Mora OA, Fernandez V, Videla LA, Junqueira VB. Enhancement of lindane-induced liver oxidative stress and hepatotoxicity by thyroid hormone is reduced by gadolinium chloride. Free Radic Res. 2002 ; 36 : 1033-9.   DOI
29 Carmeli E, Bachar A, Barchad S, Morad M, Merrick J. Antioxidant status in the serum of persons with intellectual disability and hypothyroidism: a pilot study. Res Dev Disabilities. 2008 ; 29 : 431-8.   DOI
30 Feng X, Jiang Y, Melzer P, Yen PM. Thyroid hormone regulation of hepatic genes in vivo detected by complementary DNA microarray. Mol Endocrinol. 2000 ; 14 : 947-55.   DOI
31 Laycock MA, Pascuzzi RM. The neuromuscular effects of hypothyroidism. Semin Neurol. 1991 ; 11 : 288-94.   DOI
32 Amin A, Hamza AA. Oxidative stress mediates drug-induced hepatotoxicity in rats: a possible role of DNA fragmentation. Toxicology. 2005 ; 208 : 367-75.   DOI
33 Chattopadhyay S, Sahoo DK, Subudhi U, Chainy GB. Differential expression profiles of antioxidant enzymes and glutathione redox status in hyperthyroid rats: a temporal analysis. Comp Biochem Physiol C Toxicol Pharmacol. 2007 ; 146 : 383-91.   DOI
34 Williams KV, Nayak S, Becker D, Reyes J, Burmeister LA. Fifty years of experience with propylthiouracilassociated hepatotoxicity: what have we learned? J Clin Endocrinol Metab. 1997 ; 82 : 1727-33.   DOI
35 Ahn SY. Thyroid Clinic. Seoul : Seongbosa. 2004 : 45-8, 132-59, 241-8.
36 Andreoli TE , Carpenter CJ , Plum F, Smith L. Cecil Essentials of Medicine, Philadilphia : WB Saunder's Company. 1986 : 436-7.
37 Guyton AC. Textbook of Medical Physiology. Philadelphia : WB Saunder's Company. 1986 : 906-8.
38 Schmidt RF, Thews G. Human Physiology. Berlin Heidelberg : Springer-Verlag. 1989 : 385-6.
39 Wiersinga WM. Thyroid hormone replacement therapy. Horm Res. 2001 ; 56 : 74-81.   DOI
40 Health Insurance Review & Assessment Service. Healthcare Bigdata Hub. 2013-2017. Available from : URL : http://opendata.hira.or.kr/op/opc/olap3thDsInfo.do
41 Kim DH, Kim HM, Ryu JH, Eom JY, Kim SC, Yang JH, Cho MK, IM JP, Hong SH. Herbal Pharmacology. 2nd rev. ed. Seoul : Shinilbooks. 2006 : 758-61.
42 Song JZ, Yiu HH, Qiao CF, Han QB, Xu HX. Chemical comparison and classification of Radix Astragali by determination of isoflavonoids and astragalosides. J Pharm Biomed Anal. 2008 ; 47 : 399-406.   DOI
43 Zhang ZZ, Liang XM, Zhang Q, Lu PZ. Characterization and recognition key components in Astragalus membranaceus Yao Xue Xue Bao. 2001 ; 36 : 523-7.
44 Wang RT, Shan BE, Li QX. Extracorporeal experimental study on immuno-modulatory activity of Astragalus memhranaceus extract Chinese Journal of Integrated Traditional and Western Medicine. 2002 ; 22 : 453-6.   DOI
45 Chan JY, Lam FC, Leung PC, Che CT, Fung KP. Antihyperglycemic and antioxidative effects of a herbal formulation of Radix Astragali, Radix Codonopsis and Cortex Lycii in a mouse model of type 2 diabetes mellitus. Phytother Res. 2009 ; 23 : 658-65.   DOI
46 Cho BY. Clinical Thyroidology. 2nd rev. ed. Seoul : Korea Medical Book Publishing Company. 2001 : 409-41.
47 Cui R, He J, Wang B, Zhang F, Chen G, Yin S, Shen H. Suppressive effect of Astragalus membranaceus Bunge on chemical hepatocarcinogenesis in rats. Cancer Chemotherapy and Pharmacology. 2003 ; 51 : 75-80.   DOI
48 Sun Y, Yang J. Experimental study of the effect of Astragalus membranaceus against herpes simplex virus type 1. Di Yi Jun Yi Da Xue Xue Bao. 2004 ; 24 : 57-8.
49 Kim C, Ha H, Lee JH, Kim JS, Song K, Park SW. Herbal extract prevents bone loss in ovariectomized rats. Arch Pharm Res. 2003 ; 26 : 917-24.   DOI