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http://dx.doi.org/10.6116/kjh.2019.34.1.23.

Effect of Fritillariae Thunbergii Bulbus for Allergic Inflammation on HaCaT and RBL2H3 Cells  

Kim, Eun-Young (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Lee, Bina (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Jae-Hyun (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Hong, SooYeon (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Minsun (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Park, Jae Ho (Department of Medicinal Plant Science, Jungwon University)
Kim, Jwa-Jin (Department of Medical Science, Brain Research Institute, School of Medicine, Chungnam National University)
Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.34, no.1, 2019 , pp. 23-31 More about this Journal
Abstract
Objectives : The aim of this study was to investigate the effect for allergic-inflammation of Fritillariae Thunbergii Bulbus (FTB) on HaCaT cells and RBL2H3 cells. Methods : To investigate the effects of FTB for anti-inflammation in HaCaT cells, the cells were pretreated with FTB for 1h and then stimulated with $TNF-{\alpha}/IFN-{\beta}$ for 24h. Then thymus and activation-regulated chemokine (TARC) and Macrophage-derived chemokine (MDC) levels were analyzed with ELISA kit. Also to investigate the effect of skin barrier protein, the cells were treated with FTB of various concentrations, and then cells were harvested, expressions of skin barrier protein were measured with RT-PCR. To investigate the effects of FTB for anti-allergy in RBL2H3 cells, the cells were pre-treated with FTB for 1h, and then stimulated with A23187 for 30 min. ${\beta}$-hexosaminidase, IL-4 and $TNF-{\alpha}$ were measured using cultured media. The cells were harvested to analyze the mechanism of the effect for FTB via Western blot. Results : FTB did not show cytotoxicity in HaCaT and RBL2H3. In HaCaT cells, FTB significantly suppressed the expression of TARC, MDC at a dose-dependent manner and markedly increased formation of the skin barrier proteins. In RBL2H3 cells, FTB decreased release of the ${\beta}$-hexosaminidase, IL-4 and $TNF-{\alpha}$ in RBL2H3 through inhibition of the phosphorylation of JNK and p38, which are include in the signaling mechanism of MAPK Conclusion : These results indicate that FTB has an anti-inflammatory effect on the allergic response through blocking MAPK pathway. This suggest that FTB could be a therapeutic agent for allergic response.
Keywords
Fritillariae Thunbergii Bulbus; Allergy; HaCaT; RBL2H3;
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