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http://dx.doi.org/10.6116/kjh.2018.33.6.71.

Anti-inflammatory Effects of Ethanol Extract of Chinese Medicinal Plants in Yanjin on LPS-stimulated RAW 264.7 Macrophages  

Park, Yea-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
Seo, Jong-Hwan (Department of Pharmacology, College of Korean Medicine, Sangji University)
Gil, Tae-Young (Department of Pharmacology, College of Korean Medicine, Sangji University)
Cheon, Se-Yun (Department of Pharmacology, College of Korean Medicine, Sangji University)
Piao, Ren-Zhe (College of Agriculture, Yanbian University)
Lee, Sang-Woo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Cha, Yun-Yeop (Department of Rehabilitative Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sangji University)
An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
Publication Information
The Korea Journal of Herbology / v.33, no.6, 2018 , pp. 71-78 More about this Journal
Abstract
Objectives : This study was fulfilled to investigate nominee materials as anti-inflammatory agent from ethanol extract of Chinese medicinal plants in Yanjin. Among the 20 candidates, we selected most effective one, the ethanol extract of Cicuta virosa L. (CVL). The mechanism underlying the anti-inflammatory effects of CVL is not clearly identified as yet. Accordingly, we clarified the anti-inflammatory effects of CVL and its underlying molecular mechanisms in LPS-stimulated RAW 264.7 macrophages. Methods : RAW264.7 macrophages were incubated with CVL (12.5, 25, or $50{\mu}M$) and/or lipopolysaccharide (LPS) ($1{\mu}g/m{\ell}$). Cytotoxicity was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the level of nitric oxide (NO) production was measured with Griess reagent. The prostaglandin $E_2$ ($PGE_2$) production was measured with enzyme immunoassay kits and the protein expression of inducible nitric oxide synthase (iNOS) was determined using Western blot analysis. Results : Among the 20 ethanol extract of Chinese medicinal plants of Yanjin tested, CVL significantly reduced the production of NO in a dose-dependent manner via inhibition the protein expressions of iNOS without cytotoxicity on the LPS-stimulated RAW 264.7 macrophages. In addition, CVL also effectively declined the production of $PGE_2$ in LPS-simulated RAW 264.7 macrophages. Conclusions : Taken together, these data presented in this study demonstrate that CVL possesses anti-inflammatory activity by suppressing the production of pro-inflammatory mediators NO and $PGE_2$, and pro-inflammatory protein iNOS expression in LPS-stimulated RAW 264.7 macrophages.
Keywords
Cicuta virosa L.; lipopolysaccharide; nitric oxide; prostaglandin $E_2$; inducible nitric oxide synthase;
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