Browse > Article
http://dx.doi.org/10.6116/kjh.2018.33.5.9.

Ethanol extract of medicinal herbal mixture accelerates hair growth and melanogenesis in vivo and in vitro  

Kim, Ji Yoon (Department of Beauty science of Arts, Dongbang Culture University)
Kim, Yoo-Jin (Department of Herbal Pharmacology, Daegu Haany University)
Kim, Moon Ju (Department of Beauty science of Arts, Dongbang Culture University)
Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.33, no.5, 2018 , pp. 9-18 More about this Journal
Abstract
Objectives : This study was performed to determine the transdermal effects of ethanol extract from medicinal herbal mixture (SHJ) on hair growth in C57BL/6 mice and melanogenesis in melanoma cells. Methods : Mice were divided into 3 experimental groups including vehicle (CON), SHJ extract and 5% minoxidil (MNXD, positive control)-treated group. SHJ was applied topically on the hair-shaved skin of C57BL/6 mice everyday for 15 days. The thickness and density of hair with a folliscope and morphometry of hair follicle with a H&E staining were monitored at last day. Also then, hair growth-associated gene expressions were measured by immunoblot assay. Results : The MNXD or SHJ-treated group promoted on hair growth compared to that of vehicle-treated group (CON). Hair density and thickness of MNXD or SHJ treated-group increased compared to that of vehicle application on the 15 days, respectively. Induction of insulin-like growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were also accelerated by application of SHJ extract compared to those of CON group. But expression of transforming growth factor (TGF)-${\beta}1$ decreased in SHJ treated-group compared to that of CON group. Furthermore, SHJ extract showed to increase melanin contents in a dose-dependent manner. Tyrosinase activity significantly increased in SHJ-treated group compared with CON group in dose-dependant manner. Conclusions : These results suggest that SHJ can be used as a component of cosmeceuticals for hair care via promoting growth and melanogenesis of hair.
Keywords
Medicinal herbal mixture (SHJ); IGF-1; VEGF; TGF-${\beta}1$; melanin; tyrosinase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Tobin DJ, Paus R. Graying: gerontobiology of the hair follicle pigmentary unit. Exp Gerontol. 2001 ; 36(3) : 29-54.   DOI
2 Park WS, Lee CH, Lee BG, Hang IS. The extract of Thujae occidentalis semen inhibited 5${\alpha}$-reductase and androchronogenetic alopecia of B6CBAF1/j hybrid mouse. J Dermatol Sci. 2003 ; 31(2) : 91-98.   DOI
3 Park JS, Yeom MS, Park WS, Joo KM, Rho HS, Kim DH, Chang IS. Enzymatic hydrolysis of green tea seed extract and its activity on 5${\alpha}$-reductase inhibition. Biosci Biotechnol Biochem. 2006 ; 70(2) : 387-394.   DOI
4 Liu J, Kurashiki K, Shimizu K, Kondo R. 5${\alpha}$-reductase inhibitory effect of triterpenoids isolated from Ganoderma lucidum. Biol Pharm Bull. 2006 ; 29(2) : 392-395.   DOI
5 Chaudhary UB, Turner JS. Finasteride. Expert Opin Drug Metab Toxicol. 2010 ; 6 : 873-881.   DOI
6 Hamamoto T, Mori Y. Sulfation of minoxidil in keratinocytes and hair follicles. Res Commun Chem Pathol Pharmacol. 1989 ; 66:33-44.
7 Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH. Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 2004 ; 34(2) : 91-98.   DOI
8 Mackay A, Isles C, Henderson I, Fife R, Kennedy AC. Minoxidil in the management of intractable hypertension. Quarterly J of Medicine. 1981 ; 50: 175-190.
9 Tobin DJ, Hagen E, Botchkarev VA, Paus R. Do hair bulb melanocytes undergo apoptosis during hair follicle regression catagen. J Invest Dermatol. 1998 ; 111 : 941-947.   DOI
10 Kim DH. Tech market report hair loss treatment market. Health technology transfer center. 2016 ; 2-3.
11 Cline DJ. Changes in hair color. Dermatol Clin. 1998 ; 6(2) : 295-303.
12 Dawber R and Neste DV. Hair and scalp disorders. 1st ed. London, Martin Dunitz. 1995 ; 236-238.
13 Kim DW, Shin DJ, Lee SJ, Chung SL, Kim JC. Statistical and Clinical Study of Gray Hair. Kor J Dermatol. 1999 ; 37(11) : 1567-1575.
14 Lee JO, Lee JY. A study on the periodical shape changing of hair and recognition of hair-coloring effect after hair-coloring treatment. J Kor Soc Cosm. 2008 ; 14(2) : 524-534.
15 Kim JY, Kim MR. Ethanol extract of Sinsunyukza-hwan, a Korean medicinal prescription, promotes hair growth in C57BL/5 mice, an alopecia animal model. Kor J Herbol. 2018 ; 33(3) : 1-9.   DOI
16 Hosoi J, Abe E, Suda T, and Kuroki T. Regulation of melanin synthesis of B16 melanoma cell by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 1985 ; 45 : 1474-1478.
17 Choi BW, Lee BH, Kang KJ, Lee ES, and Lee NH. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Kor J Pharmcogn. 1998 ; 29 : 237-242.
18 Yoon BY, Park HS. Research Paper : A study on the factors related to the scalp care service utilization of women. J Kor Soc Cosm. 2010 ; 16 : 1-14.
19 Messenger AG. The control of hair growth and pigmentation. In: Olsen EA. ed. Disorders of hair growth:diagnosis and treatment. New York, McGraw-Hill. 1994 ; 39-58.
20 Hageman T, Schlutter-Bhmer B, Allam JP, Bieber T, Novak N. Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution. Contact Dermatitis. 2005 ; 53:85-97.
21 Hamada K, Hirotsu S, Uchiwa H, Yamazaki S, Suzuki K. Pro-inflammatory cytokine interleukin-1${\alpha}$ is down regulated during anagen phase of hair cycle in vivo. J Dermatol Sci. 2003 ; 33(3) : 195-198.   DOI
22 Itami S, Kurata S, Takayasu S. Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-1 from dermal papilla cells. Biochem Biophys Res Commun. 1995 ; 212(3) : 977-994.
23 Hibino T, Nishiyama T. Role of TGF-${\beta}$2 in the human hair cycle. J Dermatol Sci. 2004 ; 35(1) : 9-18.   DOI
24 Lachgar S, Moukadiri H, Jonca F, Charveron M, Bouhaddioui N, Gall Y, et al. Vascular endothelial growth factor is an autocrine growth factor for hair dermal papilla cells. J Invest Dermatol. 1996 ; 106(1) : 17-23.   DOI
25 Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest. 2001 ; 107(4) : 409-417.   DOI
26 Su HY, Hickford GH, Bickerstaffe R, Palmer BR. Insulin-like growth factor 1 and hair growth. Dermatol Online J. 1999 ; 5(2) : 1.
27 Kim NH, Moon SH, Kim MR, Lee YS, Yoo WK. Haeae-tang including Artemisia argyi Folium promotes hair growth in hair-removed C57BL/6 Mice. Kor J Herbol. 2015;30(2):19-24.   DOI
28 Kang H, Kang TW, Lee SD, Park YM, Kim HO, Kim SY. The changing patterns of hair density and thickness in South Korean women with hair loss : clinical office-based phototrichogram analysis. Int J Dermatol. 2009 ; 48 : 14-21.   DOI
29 Tobin DJ, Paus R. Graying: gerontobiology of the hair follicle pigmentary unit. Exp Gerontol. 2001 ; 36(3) : 29-54.   DOI
30 Fu JM, Price VH. Approach to hairlossin women for color. Sem Cutan Med Surg. 2009 ; 28(2) : 109-114.   DOI
31 Choi YJ, Yoon TJ, Lee YH. Changing expression of the genes related to human hair graying. J Dermatol. 2008 ; 18(4) : 397-399.
32 Lee GS, Kim JJ, Song CS, Oh CG, Yim GS. Effect of Radix Trichosanthis on the melanogenesis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2001 ; 14(1) : 209-225.
33 Wolff K, Goldsmith LA, Katz SI, Gilchrest BA, Paller AS, Leffell DJ. Fitzpatrick's dermatology in general medicine. 7th ed. New York, McGraw-Hill. 2007.
34 Tanimura S, Tadokoro Y, Inomata K, Binh NT, Nishie W, Yamazaki S, Nakauchi H, Tanaka Y, McMillan JR, Sawamura D, Yancey K, Shimizu H, Nishimura EK. Hair follicle stem cells provide a functional niche for melanocyte stem cells. Cell Stem Cell. 2011 ; 8(2) : 177-187.   DOI
35 Cui R, Widlund HR, Feige E, Lin JY, Wilensky DL, Igras VE, Orazio JD, Fung CY, Schanbacher CF, Granter SR, Fisher DE. Central role of p53 in the suntan response and pathologic hyperpigmentation. Cell. 2007 ; 128(5) : 853-864.   DOI
36 Nishimura EK, Granter SR, Fisher DE. Mechanisms of hair graying: incomplete melanocyte stem cell maintenance in the niche. Science. 2005 ; 307 (5710) : 720-724.   DOI
37 Pawelek JM. After dopachrome? Pigment cell Res. 1991 ; 4 : 53-62.   DOI
38 Abdel-Malek ZA, Swope VB, Suzuki I, Harriger D, Boyce ST, Urabe K, Hearing VJ. Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc Natl Acad Sci. USA. 1995 ; 92 : 1789-1793.   DOI
39 Fuller BB, Meyskens FL. Jr. Endocrine responsiveness in human melanocyte and melanoma cells in culture. J Natl Cancer Inst. 1981 ; 66 : 799-802.
40 Leeuw SM, Smit NP, Van Veldhoven M, Pennings EM, Pavel S, Simons JW, et al. Melanin content of cultured human melanocytes and UV-induced cytotoxicity. J Photochem Photobiol B. 2001 ; 61(3) : 106-113.   DOI
41 Lee JH. Effect of Cynanchi wilfordii Radix, Lyci Fructus, Hovenia dulcis Extracts on hair growth in C57BL/6 mice. Kyung Hee University Master's degree, Seoul, Korea. 2014.
42 Randall VA. Androgens and human hair growth. Clinical Endoclinology. 1994 ; 40(4) : 439-457.   DOI
43 Headington JT. Histological findings in androgenic alopecia treated with topical minoxidil. Br J Dermatol. 1982 ; 107(22) : 20-21.
44 Lee HH, Jung MY, Kim SJ. Scalp & Medical treatment. Hyunmoon Co. Seoul, Korea. 2015 ; 125-135.
45 Do EJ, Hwang MY, Kim SY, Lee JS, Yang DS, Yang CH, Kim MR. The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice. Kor J Herbol. 2011 ; 26(4):9-14.   DOI