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http://dx.doi.org/10.6116/kjh.2018.33.1.1.

Investigation of the differential effect of juice or water extract from Puerariae Radix on hair growth related-genes in dermal papilla cells  

Jeon, Haili (Department of Biochemistry, Chungnam National University)
Cho, Namjoon (Department of Biochemistry, Chungnam National University)
Kim, Keekwang (Department of Biochemistry, Chungnam National University)
Han, Hyosang (Department of Health Administration, College of Social Sciences, Joongbu University)
Publication Information
The Korea Journal of Herbology / v.33, no.1, 2018 , pp. 1-7 More about this Journal
Abstract
Objectives : Puerariae Radix extracts are a rich source of isoflavones that affect the activity of hepatic antioxidant enzymes. However, the effect of these extracts on hair growth in human dermal papilla (DP) cells is unknown. The purpose of this study is to compare the effects between juice and water extracts of Puerariae Radix on the mRNA expression levels of hair growth-related genes in dermal papilla (DP) cells. Methods : The antioxidant activity of juice and water extracts of Puerariae Radix was analyzed using an ABTS assay. The cytotoxicity was analyzed using the MTS assay in DP cells. mRNA expression levels of hair growth-related genes in dermal papilla (DP) cells were analyzed using quantitative RT-PCR analysis. Results : Juice and water extracts of Puerariae Radix showed strong antioxidant activity. The cytotoxicity was confirmed to be higher in the juice extract than the water extract, using the MTS assay on DP cells. The mRNA expression levels of CTNNB1, FGF7, and BMP6 were significantly increased after treatment with water extract, whereas the juice extract did not affect the expression of hair growth-related genes. Conclusions : Our study provides evidence that water extract of Puerariae Radix is effective at inducing hair growth, by promoting the mRNA expression levels of hair growth-related genes. Also in the future, studies should be conducted to investigate the effects of Puerariae radix extracts on the various hair growth mechanisms of dermal papilla cells.
Keywords
Purerariae radix; Extraction; Dermal papilla cell; Antioxidant effect; Hair growth;
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Times Cited By KSCI : 4  (Citation Analysis)
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