Browse > Article
http://dx.doi.org/10.6116/kjh.2017.32.6.1.

Evaluation of Antioxidant and Anti-diabetic Effects of Sappan Lignum by Extraction Method  

Hong, Young Ju (Department of Food Science and Biotechnology, Daegu University)
Jeong, Gyeong Han (Department of Food Science and Biotechnology, Daegu University)
Jeong, Yun Hee (Department of Food Science and Biotechnology, Daegu University)
Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
Publication Information
The Korea Journal of Herbology / v.32, no.6, 2017 , pp. 1-7 More about this Journal
Abstract
Objectives : The heartwood of Sappan Lignum has been used since ancient times as an ingredient in folk medicines against anti-bacterial and anti-anemia purposes. Many bioactive constituents have been derived from this biomass such as chalcones and homoisoflavonoids. In the current investigation, the antioxidant and anti-diabetic properties using DPPH and $ABTS^+$ radicals scavenging, ${\alpha}-glucosidase$, and advanced glycation end products (AGEs) inhibition assays were evaluated by different extraction methods of Sappan Lignum. Methods : In our continuing investigation for bioactive natural ingredients, the antioxidant and ${\alpha}-glucosidase$ inhibitory properties of Sappan Lignum extracts were prepared from different extraction methods and the biological efficacies were investigated in vitro. The antioxidant properties were evaluated employing radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. In addition, the anti-diabetic effects of Sappan Lignum extracts were tested via ${\alpha}-glucosidase$ and AGEs formation inhibitory assay. The total phenolic contents were determined using a spectrophotometric method. Results : All the tested samples showed dose-dependent radical scavenging and ${\alpha}-glucosidase$ inhibitory activities. Among the tested extracts, the 80% methanolic extract of Sappan Lignum was showed the most potent activity with an $IC_{50}$ value of $82.3{\pm}1.7{\mu}g/m{\ell}$ against DPPH radical scavenging assay. While, $ABTS^+$ radical scavenging activity of 80% methanolic extract was higher than those of other extracts. Also, ${\alpha}-glucosidase$ inhibitory and AGEs formation effects of each extacts and total phenolic contents were evaluated. Conclusions : These results suggested that Sappan Lignum can be considered as a new effective source of natural antioxidant and anti-diabetic materials.
Keywords
Sappan Lignum; DPPH; $ABTS^+$; ${\alpha}-glucosidase$; advanced glycation end products (AGEs);
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Shim JS, Kim SD, Kim TS, Kim KN. Biological activities of flavonoid glycosides isolated from Angelica keiskei . Korean J Food Sci Technol. 2005 ; 37(1) : 78-83.
2 Farag RS, Badei AZMA, Hewedi GSA, Baroty EL. Antioxidant activity of some spice essential ols on linoleic acid osidation in aqueous media. J American Oil Chem Soc. 1984 ; 66(6) : 792-799.   DOI
3 Frei B. National antioxidants in human health and disease. Academic Press, San Diego. 1944 ; 44-55.
4 Branen AL. Toxicology and biochemistry of butylated hydroxy anisole and butylated hydroxy toluene. J Oil Chem Soc. 1975 ; 52(2) : 59-62.   DOI
5 Maltese F, Erkelens C, van der Kooy F, Choi YH, Verpoorte R. Identification of natural epimeric flavanone glycosides by NMR spectroscopy. Food chem. 2009 ; 116(2) : 575-579.   DOI
6 DeFronzo RA, Bonadonna RC, Ferrannini E. Pathogenesis of NIDDM: a balanced overview. Diabetes care. 1992 ; 15(3) : 318-368.   DOI
7 Kahn SE, Prigeon R.L, McCulloch DK, Boyko EJ, Bergman RN, Schwartz MW, Palmer JP. Quantification of the relationship between insulin sensitivity and ${\beta}$-cell function in human subjects: evidence for a hyperbolic function. Diabetes care. 1993 ; 42(11) : 1663-1672.   DOI
8 Videla LA, Fermandez V. Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp. 1988 ; 21(1) : 85-92.
9 Fineman MS, Bicsak TA, Shen LZ, Taylor K, Gaines E, Varns A, Baron AD. Effect on glycemic control of exenatide (synthetic exendin-4) additive to existing metformin and/or sulfonylurea treatment in patients with type 2 diabetes. Diabetes Care. 2003 ; 26(8) : 2370-2377.   DOI
10 Robertson RP, Harmon J, Tran POT, Poitout V. ${\beta}$-cell glucose toxicity, lipotoxicity, and chronic oxidative stress in type 2 diabetes. Diabetes care. 1992 ; 53(1) : 119-124.
11 Tsujimoto T, Shioyama E, Moriya K, Kawaratani H, Shirai Y, Toyohara M, Fukui H. Pneumatosis cystoides intestinalis following alpha-glucosidase inhibitor treatment: a case report and review of the literature. World J Gastroenterol. 2008 ; 14(39) : 6087-6092.   DOI
12 Kihara Y, Ogami Y, Tabaru A, Unoki H, Otsuki M. Safe and effective treatment of diabetes mellitus associated with chronic liver diseases with an alphaglucosidase inhibitor, acarbose. J Gastroenterol. 1997 ; 32(6) : 777-782.   DOI
13 Brownlee M. The pathobiology of diabetic complications. Diabetes care. 2005 ; 54(6) : 1615-1625.   DOI
14 Tosi F, Muggeo M, Brun E, Spiazzi G, Perobelli L, Zanolin E, Moghetti P. Combination treatment with metformin and glibenclamide versus single-drug therapies in type 2 diabetes mellitus: a randomized, double-blind, comparative study. Metabolism. 2003 ; 52(7) : 862-867.   DOI
15 Oudjeriouat N, Moreau Y, Santimone M, Svensson B, Marchis‐Mouren G, Desseaux V. On the mechanism of ${\alpha}$-amylase. Eur J Biochem. 2003 ; 270(19) : 3871-3879.   DOI
16 Goldstein BJ, Pans M, Rubin CJ. Multicenter, randomized, double-masked, parallel-group assessment of simultaneous glipizide/metformin as second-line pharmacologic treatment for patients with type 2 diabetes mellitus that is inadequately controlled by a sulfonylurea. Clin Ther. 2003 ; 25(3) : 890-903.   DOI
17 Huebschmann AG, Regensteiner JG, Vlassara H, Reusch JE. Diabetes and advanced glycoxidation end products. Diabetes care. 2006 ; 29(6) : 1420-1432.   DOI
18 Vlassara H. Advanced glycation end-products and atherosclerosis. Ann Med. 1996 ; 28(5) : 419-426.   DOI
19 Matsuda H, Wang T, Managi H, Yoshikawa M. Structural requirements of flavonoids for inhibition of protein glycation and radical scavenging activities. Bioorg. Med. Chem. 2003 ; 11(24) : 5317-5323.   DOI
20 Edelstein D, Brownlee M. Mechanistic studies of advanced glycosylation end product inhibition by aminoguanidine. Diabetes care. 1992 ; 41(1) : 26-29.
21 Choi SY, Yang KM, Jeon SD, Kim JH, Khil LY, Chang TS, Moon CK. Brazilin modulates immune function mainly by augmenting T cell activity in halothane administered mice. Planta Med. 1997 ; 63(5) : 405-408.   DOI
22 Lee EB, Xing MM, Kim DK. Lifespn-exterding and stress resistance properties of brazilin from Caesalpinia sappan in Caenorhabditis elegans. Arch Pharm Res. 2017 ; 40(7) : 825-835.   DOI
23 Baek NI, Jeon SG, Ahn EM, Hahn JT, Bahn JH, Jang JS, Choi SY. Anticonvulsant compounds from the wood of Caesalpinia sappan L. Arch Pharm Res. 2000 ; 23(4) : 344-348.   DOI
24 Oh SR, Kim DS, Jung KY, Lee JJ, Lee HK. Anticomplementary activity of constituents from the heartwood of Caesalpinia sappan. Planta Med. 1998 ; 64(5) : 456-458.   DOI
25 Moon CK, Park KS, Kim SG, Won HS, Chung JH. Brazilin protects cultured rat hepatocytes from BrCCI3-induced toxicity. Drug Chem Toxicol. 1992 ; 15(1) : 81-91.   DOI
26 Hikino H, Taguchi T, Fujimura H, Hiramatsu Y. Antinflammatory principles of Caesalpinia sappan wood and of heamatoxylon campechianum wood1. Planta Med. 1977 ; 31(3) : 214-220.   DOI
27 Hung TM, Dang NH, Dat NT. Methanol extract from vietnamese Caesalpinia sappan induces apoptosis in HeLa cells. Biol Res. 2014 ; 47(1) : 20-24.   DOI
28 Kim YM, Kim SG, Khil LY, Moon CK. Brazilin stimulates the glucose transport in 3T3-L1 cells. Planta Med. 1995 ; 61(4) : 297-301.   DOI
29 Oswal VB, Garg SC. Unsaponifiable matter of the fixed oil from the seeds of Caesalpinnia sappan Linn. Asian J. Chem. 1993 ; 5(3) : 676-676.
30 Namikoshi M, Nakata H, Yamada H, Nagai M, SAITOH T. Homoisoflavonoids and related compounds. II. Isolation and absolute configurations of 3, 4- dihydroxylated homoisoflavans and brazilins from Caesalpinia sappan L. Chem Pharm Bull. 1987 ; 35(7) : 2761-2773.   DOI
31 Namikoshi M, Nakata H, Saitoh T. Homoisoflavonoids from Caesalpinia sappan . Phytochemistry. 1987 ; 26(6) : 1831-1833.   DOI
32 Nagai M, Nagumo S, Lee SM, Eguchi I, Kawai KI. Protosappanin A, a novel biphenyl compound from Sappan Lignum. Chem Pharm Bull. 1986 ; 34(1) : 1-6.   DOI
33 Badami S, Moorkoth S, Rai SR, Kannan E, Bhojraj S. Antioxidant activity of Caesalpinia sappan heartwood. Chem Pharm Bull. 2003 ; 26(11) : 1534-1537.   DOI
34 Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965 ; 16(3) : 144-158.
35 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958 ; 181(4617) : 1199-1200.   DOI
36 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS+ radical cation decolorization assay. Free Radic Biol Med. 1999 ; 26(9) : 1231-1237.   DOI
37 Eom SH, Lee SH, Yoon NY, Jung WK, Jeon YJ, Kim SK, Kim YM. ${\alpha}$-Glucosidase and ${\alpha}$-amylase‐inhibitory activities of phlorotannins from Eisenia bicyclis. J Sci Food Agric. 2012 ; 92(10) : 2084-2090.   DOI
38 Vinson JA, Howard TB. Inhibition of protein glycation and advanced glycation end products by ascorbic acid and other vitamins and nutrients. J. Nutr. Biochem. 1996 ; 7(12) : 659-663.   DOI
39 Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance, Nutr Rev. 1998 ; 56(11) : 317-333.   DOI
40 Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial byproducts: Antioxidant activity, occurrence, and potential uses. Food Chem. 2006 ; 99(1) : 191-203.   DOI
41 Lee SG, Yu MH, Lee SP, Lee IS. Antioxidant activities and induction of apoptosis by methanol extracts from avocado. J Korean Soc Food Sci Nutr. 2008 ; 37(3) : 269-275.   DOI
42 Shin JA, Lee JH, Kim HS, Choi YH, Cho JH, Yoon KH. Prevention of diabetes: a strategic approach for individual patients. Diabetes Metab Res Rev. 2012 ; 28(2) : 79-84.   DOI
43 Bischoff H. The mechanism of alpha-glucosidase inhibition in the management of diabetes. Clin Invest Med. 1995 ; 18(4) : 303-311.
44 Jennings PE, Barnett AH. New approaches to the pathogenesis and treatment of diabetic microangiopathy. Diabetic Med. 1988 ; 5(2) : 111-117.   DOI
45 Halliwell B, Aruoma OJ. DNA damage by oxygenderived species. FEBS Lett, 1991 ; 281(1-2) : 9-19.   DOI