Browse > Article
http://dx.doi.org/10.6116/kjh.2017.32.3.1.

Effect of Scrophulariae Radix buergeriana Water Extract on the Proinflammatory Mediators in RAW 264.7 Cells Induced by LPS  

Han, Hyo-Sang (Department of Health Administration, College of Tourism Health, Joongbu University)
Publication Information
The Korea Journal of Herbology / v.32, no.3, 2017 , pp. 1-7 More about this Journal
Abstract
Objective : The purpose of this study was to investigate the effects of Scrophulariae Radix Water Extract (SR) on the production of inflammatory mediators in RAW 264.7 mouse macrophages cells induced by lipopolysaccharide (LPS). Method : We examined effect of Scrophulariae Radix Extract on the cell viability of mouse macrophages cells. Futhermore, After 24 hours treatment we investigated anti-inflammatory effect of Scrophulariae Radix Extract by the production of Bio-Plex cytokine assay, concentrations of various cytokines such NO, $interleukin(IL)-1{\alpha}$, IL-3 and interferon inducible protein-10(IP-10). Result : No significant changes have been found in the mouse macrophge cell viability by the Scrophulariae Radix Extract at the concentration of 25, 50, 100 and $200{\mu}g/m{\ell}$. The water extract of Scrophulariae Radix significantly inhibited the production of NO in the LPS-induced macrophage at the concentration of 25, 50, 100 and $200{\mu}g/m{\ell}$. The water extract of Scrophulariae Radix significantly inhibited the production of $IL-1{\alpha}$, IL-3 and IP-10 in the LPS-induced macrophage at the concentration of 50, 100 and $200{\mu}g/m{\ell}$. Conclusion : The water extract of Scrophulariae Radix significantly inhibited the production of NO, $IL-1{\alpha}$, IL-3 and IP-10 at the concentration of $50{\mu}g/m{\ell}$ or higher in the LPS-induced macrophages with no changes in the cell viability of them. These results suggest that water extract of Scrophulariae Radix has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as $IL-1{\alpha}$, IL-3 and IP-10 in the LPS-induced macrophages.
Keywords
Scrophulariae Radix; macrophage; anti-inflammatory effect; cytokine; nitric oxide;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Ahmed B, Al-Rehaily AJ, A1-Howiriny TA, El-Sayed KA, Ahmad MS. Scropolioside-D2 and harpagoside-B: two new iridoid glycosides and antiinflammatory activity. Biol. Pharm. Bull. 2003 ; 26(4) : 462-7.   DOI
2 Lee EJ, Kim SR, Kim J, Kim YC. Hepatoprotective Phenylpropanoids from Scrophularia buergeriana roots against CCl4-Induced toxicity: Action mechanism and structure-activity relationship. Planta Med. 2002 ; 68(5) : 407-11.   DOI
3 Tohda C, Kakihara Y, Komatsu K, Kuraishi, Y. lnhibitory effects of methanol extracts of herbal medicines on substance P-induced itch-scratch response. Biol. Pharm. Bull. 2000 ; 23(5) : 599-601.   DOI
4 Byun, SH, Yang CH, Kim SC. Inhibitory effect of Scrophulariae Radix extract on TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and Nitric Oxide production in Lipopolysaccharide - activated Raw 264.7 cells. J of Herbology. 2005 ; 20(2) : 7-16.
5 Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 ; 65(1-2) : 55-63.   DOI
6 Yeo EJ, HAN JK, Kim YH. Inhibitory Studies of Scrophulariae Radix on Development of Dermatitis in NC/Nga Mice. J Korean Oriental Pediatrics. 2007 ; 21(2) : 13-34.
7 Politch JA, Tucker L, Bowman FP, Anderson DJ. Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men. Hum Reprod. 2007 ; 22(11) : 2928-35.   DOI
8 Kim DH, Kim HM, Ryu JH, Eom JY, Kim SC, Yang JH, Cho MK, Lim JP, Hong SH. Hanbangyakrihak. Seoul : Shinilbukseu. 2007 : 693-5.
9 Lee YT. Choesinmyeonyeokhak. Seoul : Jipmundang. 1989 : 355-38.
10 Cohen J. The immunopathogenesis of sepsis. Nature. 2002 ; 420(6917) : 885-91.   DOI
11 Kim HS. The Cytokines : An Overview. Yeungnam Univ. J of Med. 2010 ; 27(1) : 1-7.   DOI
12 Cho HY, Noh KH, Cho MK, Jang JH, Lee MO, Kim SH, Song YS. Anti-oxidative and Anti-inflammatory Effects of Genistein in BALB/c Mice Injected with LPS. J Kor Soc Food Sci Nutr. 2008 : 37(9) : 1126-35.   DOI
13 Nagata S, Tsuchiva M, Asano S, Kaziro Y, Tamazaki T, Yamamoto O, Hirata Y, Kubota N, Oheda M, Nomura H, Ono M. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature. 1986 ; 319(6052) : 415-8.   DOI
14 Feldman PL, Griffith OW, Sheuhr DJ. The surprising life of nitric oxide. Chem Eng News. 1993 : 26-38.
15 Nazarenko I, Marhaba R, Reich E, Voronov E, Vitacolonna M, Hildebrand D, Elter E, Rajasagi M, Apte RN, Zo, ller M. Tumorigenicity of IL-1alpha-and IL-1beta-deficient fibrosarcoma cells. Neoplasia. 2008 ; 10(6) : 549-62.   DOI
16 Abul KA, Andrew HL. Cellular and Molecular Immunology. Saunders. 2003 : 243-74.
17 Agostini C, Cassatella M, Zambello R, Trentin L, Gasoerini S, Perin A, Piazza F, Siviero M, Facco M, Dziejman M, Chilosi M, Qin SX, Luster A, Semenzato G. Involvement of IP-10 chemokine in sarcoid granulomatous reactions. J Immunol. 1998 ; 161(11) : 6413-20.
18 Agostini C, Facco M, Siviero M, Carollo D, Galvan S, Cattelan AM, Zambello R, Trentin L, Semenzato G. CXC chemokines IP-10 and mig expression and direct migration of pulmonary CD8+/CXCR3+ T cells in the lungs of patients with HIV infection and T-cell alveolitis. Am J Respir Crit Care Med. 2000 ; 162(4 Pt 1) : 1466-73.   DOI
19 Matsuda H, Morikawa T, Ando S, Toguchida I, Yoshikawa M. Structural requirements of flavonoids for nitric oxide production inhibitory activity and mechanism of action. Bioorg Med Chem. 2003 ; 11(9) : 1995-2000.   DOI
20 Moon TC, Chung KC, Son KH, Kim HP, Kang SS, Chang HW. Screening of Cyclooxygenase-2(COX-2) Inhibitors from Natural Products. Yakhak Hoeji. 1998 ; 42(2) : 214-9.
21 Calixto JB, Campos MM, Otuki MF, Santos AR. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion melecules. Planta Med. 2004 ; 70(2) : 93-103.   DOI
22 Yook KD. Antimicrobial activity and cytotoxicity test of Scrophularia ningpoensis hemsl extracts against Klebsiella pneumoniae. Journal of The Korea Society of Computer and Information. 2016 ; 21(5) : 135-9.   DOI
23 Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, LeeYC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY,. Boncho-Hak. Seoul : Young-Lim Press. 2007 : 233-4.
24 Korea Food and Drug Administration. The Korean Pharmacopoeia Eleventh Edition. Seoul : Korea Food and Drug Administration. 2015 : 118.
25 Choi YH, Jung JW. Anxiolytic-like Effects of Scrophularia buergeriana Miquel Using the Elevated Plus-Maze in Mice : Involvement of GABAergic Nervous System. Korean J Oriental Physiology & Pathology. 2010 ; 24(3) : 476-83.
26 Park KH, Ham IH, Choi HY. Hypotensive Effect of Scrophularia buergeriana and Gene Analysis of Scrophularia Species. J of Herbology. 2008 ; 23(2) : 213-23.
27 Song JS, Lee JC, Choi JH, Kim JH, Park SY. Effects of Scrophulariae Radix (SR) on Allergic Contact Dermatitis (ACD) induced by DNCB in mice. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2011 ; 24(3) : 1-16.
28 Daehanbyeongrihakhoe. Byeongrihak. Seoul : Komoonsa 1995 : 71-104.
29 Park YK, Kang BS. Effects of Scrophulariae radix on Antioxidation in Rat's live. J of Herbology. 1998 ; 13(1) : 201-20.
30 Park CU, Cho CS, Kim CJ. The Effects of Radix Scrophulariae on the Hyperthyroidism of Rats. J Korean Oriental Med. 2006 ; 27(3) : 178-186.
31 Kim SR, Kang SY, Lee KY, Kim SH, Markelonis GJ, Oh TH, Kim YC. Anti-amnestic activity of E-Pmethoxycinnamic acid from Scrophularia buergeriana. Cognitive Brain Research. 2003 ; 17(2) : 454-61.   DOI