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http://dx.doi.org/10.6116/kjh.2016.31.1.33.

Hepatoprotective effect of Paeoniae radix via Nrf2 activation  

Lee, Soo Hwan (College of Oriental Medicine, Daegu Haany University)
Jung, Ji Yun (College of Oriental Medicine, Daegu Haany University)
Park, Sang Mi (College of Oriental Medicine, Daegu Haany University)
Jegal, Kyung Hwan (College of Oriental Medicine, Daegu Haany University)
Byun, Sung Hui (College of Oriental Medicine, Daegu Haany University)
Cho, Il Je (College of Oriental Medicine, Daegu Haany University)
Kim, Sang Chan (College of Oriental Medicine, Daegu Haany University)
Kim, Kwang Joong (College of Oriental Medicine, Daegu Haany University)
Kim, Young Woo (College of Oriental Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.31, no.1, 2016 , pp. 33-40 More about this Journal
Abstract
Objectives : Liver is one of the largest organs in the human, and has a function of detoxification and energy sensing to prevent severe disease. Paeoniae radix has been used to treat a variety of liver diseases such as hepatitis and chronic hepatic failure. Although P. radix has been used as an medicinal herb for a long time, the effects of P. radix on severe oxidative stress and its action mechanism on the liver was not clearly verified.Methods : This study investigated the protective effects of P. radix extract (PRE), and the underlying mechanism of its action in the liver. tert-butyl hydroperoxide (t-BHP) and carbon tetrachlroride (CCl4) were used to induce oxidative stress in the HepG2 hepatocyte cell line and Sprague-Dawley rats, respectively.Results : t-BHP significantly induced cell death and ROS production in HepG2 cell, as indicated by MTT and FACS analysis. However, pretreatment of PRE inhibited a decrease in cell viability and H2O2 production in the HepG2 cells. PRE also blocked the ability of t-BHP to damage in mitochondrial membrane transition. More importantly, PRE induced Nrf2 activation and antioxidant Phase II enzyme, which may have a role in the effects of PRE. In mice, PRE inhibited the liver damage induced by CCl4.Conclusions : PRE inhibited oxidative stress and hepatic damages as mediated with Nrf2 activation. This study unveil, in part, the effect and mechanism of old medicinal herb, P. radix.
Keywords
Paeoniae radix; Oxidative stress; Nrf2 activation; Liver;
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Times Cited By KSCI : 3  (Citation Analysis)
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