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http://dx.doi.org/10.6116/kjh.2015.30.3.69.

Effect of Mixture extracted from Bupleuri Radix and Physalidis Herba on the LPS-induced depression in Rats  

Lee, Won-Haeng (Hwa Jeop Mong HAN Clinic)
Kim, Do-Hoon (Dept. of Oriental Medical Classics and History, Gachon University)
Lee, Tae Hee (Dept. of Formulae Pharmacology. Gachon University)
Publication Information
The Korea Journal of Herbology / v.30, no.3, 2015 , pp. 69-76 More about this Journal
Abstract
Objectives : The effect of mixture extracted from Bupleuri Radix and Physalidis Herba(BR+RH) on the LPS-induced Depression in rats was investigated. Methods : Rats were administered intragastrically BR+PH after injectio of LPS to induce deprssion. Immobility was examined using Tail Suspension Test(TST), Forced Swimming Test(FST). The level of plasma corticosterone was measured by an Enzyme-Linked Immunosorbent Assay(ELISA) method. The expressions of c-Fos, Corticotropin Releasing Factor(CRF), NADPH-d in the Paraventricular nucleus(PVN) and TH in the Locus coeruleus(LC) were measured by immunohistochemical method. Results : In the effect of BR + PH on TST, immobility was significantly decreased comparing with the LPS group. In FST, immobility was shown decrease tendency in the BR+PH group. The expression of c-Fos in the PVN was significantly decreased at BR + PH400 group, comparing with the LPS group. The expression of CRF in PVN was shown dto have the decrease tendency in the BR+PH group, comparing with the LPS group. The expression of NADPH-d in PVN was not significantly decreased at BR+PH groups, comparing with the LPS group. The expression of TH in the LC was shown to have the decrease tendency at BR + RH groups, but not significantly, comparing with the LPS group. Conclusions : Anti-depressant effect of mixture after extracted from Bupleuri Radix and Physalidis Herba was through the anti-inflammatory effect via inhibition of HPA axis. NO and catecholamine system is not involved.
Keywords
Bupleuri Radix; Physalidis Herba; LPS; anti-depressant; HPA axis;
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Times Cited By KSCI : 2  (Citation Analysis)
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