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http://dx.doi.org/10.6116/kjh.2015.30.2.43.

Effect of Hoelen Cum Radix on learning and memory enhancement via stimulation of neuronal differentiation in the hippocampus of the mouse brain  

Choi, Jin Gyu (Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University)
Sim, Yeomoon (Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University)
Kim, Wonnam (Department of Internal Medicine, Section of Endocrinology, Yale School of Medicine)
Kim, Sun Yeou (College of Pharmacy, Gachon University)
Oh, Myung Sook (Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.30, no.2, 2015 , pp. 43-48 More about this Journal
Abstract
Objectives : The aim of this study was to investigate the memory enhancing properties of extract of Hoelen Cum Radix (HCR) and its possible mechanism in mice of normal condition. Methods : We evaluated the effects of HCR on cognitive function and memory enhancement in normal mice. Male ICR mice were orally administrated with HCR 100 mg/kg for 7 days and equal volume of saline was administrated to the control group in the same condition. We conducted two behavioral tests which measure the spatial working memory (Y-maze test) and cognitive fear memory (passive avoidance test). We also investigated whether HCR affects the hippocampal neurogenesis in the brain. To assess the effects of HCR on neural progenitor cell differentiation and neurite outgrowth in the early stage of hippocampal neurogenesis, we performed doublecortin (DCX), a direct neurogenesis marker, immunohistochemical analysis in the dentate gyrus (DG) of the mouse hippocampus. Results : HCR significantly enhanced memory and cognitive function as determined by the Y-maze test (p<0.05) and passive avoidance test (p<0.001). Moreover, HCR increased DCX positive cells (p<0.01) and neurite length (p<0.01) compared to the control group. These results indicated that HCR stimulates differentiation of neural progenitor cells and promotes neurite outgrowth in hippocampal DG of the mice. Conclusion : We concluded that HCR shows memory enhancing effects through the stimulation of hippocampal neurogenesis as a consequence of accelerated neuronal differentiation and neurite outgrowth in the DG of the hippocampus after HCR treatment.
Keywords
Hoelen Cum Radix; memory and cognitive function; neuronal differentiation; neurite outgrowth;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Rao MS, Shetty AK. Efficacy of doublecortin as amarker to analyse the absolute number and dendritic growth of newly generated neurons in the adult dentate gyrus. Eur J Neurosci. 2004 ; 19(2) : 234–6.   DOI   ScienceOn
2 Izquierdo I, Medina JH, Izquierdo LA, Barros DM, De Souza MM, Mello e Souza T. Short-and long-term memory are differentially regulated by monoaminergic systems in the rat brain. Neurobiol Learn Mem. 1998 ; 69(3) : 219–24.   DOI   ScienceOn
3 Professors of Korean Medicinal Physiology. Physiology of Eastern Medicine. Seoul : Jipmoondang. 2008 : 253.
4 Sarter M, Bodewitz G, Stephens DN. Attenuation of scopolamine induced impairment of spontaneous alteration behaviour by antagonist but not inverse agonist and agonist beta-carbolines. Psychopharmacology (Berl). 1988 ; 94(4) : 491-5.   DOI
5 Elrod K, Buccafusco JJ. An evaluation of the mechanism of scopolamine-induced impairment in two passive avoidance protocols. Pharmacol Biochem Behav. 1988 ; 29(1) : 15-21.   DOI   ScienceOn
6 Deng W, Aimone JB, Gage FH. New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory?. Nat Rev Neurosci. 2010 ; 11(5) : 339-50.   DOI   ScienceOn
7 Kitamura T, Inokuchi K. Role of adult neurogenesis in hippocampal-cortical memory consolidation. Mol Brain. 2014 ; 7 : 13.   DOI   ScienceOn
8 Yu GR, Chang GT, Kim JH. A literature study of Jianwang(健忘) : Forgetfulness(Amnesia). J Pediatr Korean Med. 1999 ; 13(1) : 1-16.
9 Choi YJ, Seong GG, Mun BS. A bibliographic study on the types of differential diagnosis of amnesia. J Korean Med. 1996 ; 17(1) : 374-406.
10 Ming GL, Song H. Adult neurogenesis in the mammalian central nervous system. Annu Rev Neurosci. 2005 ; 28 : 223-50.   DOI   ScienceOn
11 Jegal KH, Park SJ, Kim CY, Lee C, Park JH, Jang JH. Effect of Poria Cocos on the scopolamine-induced memory impairment and its underlying molecular mechanism. Kor J Ori Med Physiol Pathol. 2010 ; 24(2) : 228-35.
12 Korean medicinal botany research society. Medicinal botany. 2th rev. ed. Seoul : Hakchangsa. 2003 : 129.
13 Pharmacology of Oriental Herbs textbook compilation committee. Pharmacology of Oriental Herbs. 8th rev. ed. Seoul : Shinilbooks. 2010 : 657-9.
14 Hwang RI, Lim JY, Lee YW. A Comparison of the Factors Influencing the Life Satisfaction of the Elderly According to their Cognitive Impairment Level. J Korean Acad Nurs. 2009 ; 39(5) : 622-31.   DOI   ScienceOn
15 Yanhong O, Chandra M, Venkatesh D. Mild cognitive impairment in adult: a neuropsychological review. Ann Indian Acad Neurol. 2013 ; 16(3) : 310-8.   DOI   ScienceOn
16 Evans DA, Funkenstein HH, Albert MS, Scherr PA, Cook NR, Chown MJ, Hebert LE, Hennekens CH, Taylor JO. Prevalence of Alzheimer's disease in a community population of older persons. Higher than previously reported. JAMA. 1989 ; 262(18) : 2551-6.   DOI   ScienceOn
17 Nordberg A. Mechanisms behind the neuroprotective actions of cholinesterase inhibitors in Alzheimer disease. Alzheimer Dis Assoc Disord. 2006 ; 20(2) : 12-8.   DOI
18 Birks J. Cholinesterase inhibitors for Alzheimer’s disease. Cochrane Database Syst Rev. 2006 ; 25(1) : CD005593.
19 Nilsson M, Perfilieva E, Johansson U, Orwar O, Eriksson PS. Enriched environment increases neurogenesis in the adult rat dentate gyrus and improves spatial memory. J Neurobiol. 1999 ; 39(4) : 569-78.   DOI
20 Gug YJ, Choi H, Kim TH, Kang HW, Lyu YS. Chongmyung-tang Inhibits the Cytotoxicity of Beta-amyloid in Neuro 2A Neuroblastoma Cells. Korean J Orient Physiol Pathol. 2004 ; 18(5) : 1418-25.
21 Oh YJ and Kim BK. A study of ChongMyungTang(CMT) and HyangbujaChongMyungTang(HCMT) on Dementia - Extract & Nano Powder Drug types. J Oriental Neuropsychiatry. 2006 ; 17(1) : 79-105.
22 Ha SY, Jung IH, Lee SR. The Effects of ChongMyungTang(CMT) and SanSaChongMyungTang(SCMT) on the Alzheimer's Disease Model. J Orient Neuropsychiatry. 2006 ; 17(1) : 59-78.
23 Park JU, Jung IH, Lee SR. The Effects of ChongMyungTang(CMT) and ChongMyungTang added Hibiscus syriacus(MCMT) Extract on the Alzheimer's Disease Model Induced by CT-105 and βA. J Orient Neuropsychiatry. 2006 ; 17(1) : 37-57.
24 Kim KY, Kim HW, Lee SY, Cha DY, Lee SJ, Kim GY, Kim HJ, Jeong HW. Neuroprotective Effects of Gagam-ChongMeong-Tang on Cognitive Function after Ischemic Brain Injury in Rats. Korean J Oriental Physiology & Pathology. 2008 ; 22(3) : 556-61.
25 An KY, Lee SK, Lee SH, Lee JW, Shin JB, Song BK, Lee EJ. Neuroprotective Effect of Gagamchongmyung-tang on the Deficits of Learning and Memory by MCAO in the Rat. J Korean Oriental Med. 2007 ; 28(2) : 1-12.
26 Lee SR, Kim BK. Effects of Ansinchengnoy-tang on the Biochemical Changes and Memory of the Alzheimer's Disease Model. Korean J Orient Med Physiol Pathol. 2001 ; 15(5) : 708-22.
27 Koh TJ, Lee SR. Effects of Boyikcheungnoy-tang (BYCNT) on inhibition of impairment of learning and memory, and acetylcholinesterase in amnesia mice. J Orient Neuropsychiatry. 2001 ; 12(1) : 151-67.
28 Hwang DY. Bangyakhappyeon. Seoul : YounglimSa. 2002 : 533.
29 Couillard-Despres S, Winner B, Schaubeck S, Aigner R, Vroemen M, Weidner N, Bogdahn U, Winkler J, Kuhn HG, Aigner L. Doublecortin expression levels in adult brain reflect neurogenesis. Eur J Neurosci. 2005 ; 21(1) : 1-14.   DOI   ScienceOn
30 Van Praag H, Schinder AF, Christie BR, Toni N, Palmer TD, Gage FH. Functional neurogenesis in the adult hippocampus. Nature. 2002 ; 415(6875) : 1030–4.   DOI   ScienceOn
31 Hwang YK, Lee SY, Yoon HD, Shin OC, Park CG, Park CS. Effect of the neuroprotection and antiAlzheimer's disease in CT99-induced Neuro 2A cells by Ikgiansintang water extract. The Korean J Orient Med Prescription. 2005 ; 13(1) : 103-21.
32 Hattori T, Hayashi K, Nagao T, Furuta K, Ito M, Suzuki Y. Studies on antinephritic effects of plant components (3): Effect of pachyman, a main component of Poria cocos Wolf on original type anti-GBM nephritis in rats and its mechanisms. Jpn J Pharmacol. 1992 ; 59(1) : 89-96.   DOI
33 Seo BI, Kwon DY, Choi HY, Lee JH, Oh MS, Bu YM. Medicinal Herbology. 8th rev. ed. Seoul : YounglimSa. 2012 : 409-11.
34 You Q, Wang P. Study on Sedative-Hypnotic Effect of Poria Cocos and Fushen Water Decoction. J Hubei University of Chinese Medicine. 2013 ; 15(2) : 15-7.
35 Lee DI, Ko ST, Moon YH. Diuretic action of Hoelen in the dog. J Pharm Soc Kor. 1974 ; 18(1) : 39-48.
36 Feng YL, Lei P, Tian T, Yin L, Chen DQ, Chen H, Mei Q, Zhao YY, Lin RC. Diuretic activity of some fractions of the epidermis of Poria cocos. J Ethnopharmacol. 2013 ; 150(3) : 1114-8.   DOI   ScienceOn
37 Li TH, Hou CC, Chang CL, Yang WC. Anti-Hyperglycemic Properties of Crude Extract and Triterpenes from Poria cocos. Evid Based Complement Alternat Med. 2011 ; 14(1) : 101-8.
38 Yun HJ, Cha HM, Kim SW, Shin WC, Kim HG, Choe SY. Effects of the Extract of Hoelen on Serum Lipid Profiles in Mice. J Korean Soc Food Sci Nutr. 2006 ; 35(8) : 1005-9.   DOI   ScienceOn