Browse > Article
http://dx.doi.org/10.6116/kjh.2015.30.1.1.

Anti-Diabetic Effects of Mori Folium Extract on High-Fat Diet and Streptozotocin-Induced Type II Diabetes Mellitus in Mice  

Kwon, Tae-Oh (College of Life Science and Natural Resources, Wonkwang University)
Choi, Ji-Won (Department of Radiological Sciences, Jeonju University)
Lee, Hyun-Seo (Department of Health & Science, Jeonju University)
Cho, Byoung-Ok (Ato Q&A Corporation)
Yin, Hong-Hua (Ato Q&A Corporation)
Jang, Seon-Il (Department of Health & Science, Jeonju University)
Publication Information
The Korea Journal of Herbology / v.30, no.1, 2015 , pp. 1-9 More about this Journal
Abstract
Objectives : The present study was designed to investigate the anti-diabetic effects of Mori Folium (Morus alba L. of Moraceae) extract (MFE) on high fat diet (HFD) and streptozotocin (STZ)-induced type II diabetes mellitus in mice. Methods : The mice (C57BL/6J) were fed HFD for 8 weeks and then was induced with a single injection of STZ (75 mg/kg). The diabetic mice were divided into four groups [(STD, HFD, HFD + MFE and HFD + quercetin (QUR)] and administered with MFE or OUR for 4 weeks. Fasting blood glucose, lipid profile (triglycerides and cholesterol etc.), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), insulin and leptin were measured every 2 weeks. Results : Body weight gain was lower in the MFE and QUR groups than HFD group. The fasting blood glucose was lower in the MFE and QUR groups. Oral glucose and insulin tolerance were decreased in the MFE and QUR groups. The levels of serum total cholesterol, triglycerides, and LDL cholesterol were reduced in the MFE and QUR groups. The HDL cholesterol was much higher in the MFE and QUR groups than HFD group. The levels of GOT, GPT and atherogenic index were decreased in the MFE and QUR groups. The serum insulin and leptin concentrations were reduced in the MFE and QUR groups. Conclusions : These results showed that MFE could decrease blood glucose level and lead to an amelioration in dyslipidemia states on HFD/STZ-induced type II diabetes mellitus in mice.
Keywords
Mori Folium extract; Quercetin; Anti-diabetic effects; Blood glucose level; Type II diabetes mellitus;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Cowley MA, Smith RG, Diano S, Tschop M, Pronchunk N, Grove KL, Strasburger CJ, Bidlingmaier M, Esterman M, Heiman ML, Garcia-Segura LM, Nillni EA, Mendez P, Low MJ, Sotonyi P, Friedman JM, Liu H, Pinto S, Colmers WF, Conel RD, Horvath TL. The distribution and mechanism of action of ghrelin in the CNS demonstrates a novel hypothalamic circuit regulating energy homeostasis. Neuron. 2003 ; 37(4) ; 649-61.   DOI   ScienceOn
2 Klok MD, Jakobsdottir S, Drent ML. The role of leptin and ghrelin in the regulation of food intake and body weight in humans: a review. Obes Rev. 2007 ; 8(1) : 21-34.   DOI   ScienceOn
3 Kim SG, Choi DS. Epidemiology and Current Status of Diabetes in Korea. Hanyang Med Rev. 2009 ; 29(2) : 122-9.
4 Sunil C, Duraipandiyan V, Agastian P, Ignacimuthu S. Antidiabetic effect of plumbagin isolated from Plum-bago zeylanica L. root and its effect on GLUT4 translocation in streptozotocin-induced diabetic rats. Food Chem Toxicol. 2012 ; 50(12) : 4356-63.   DOI   ScienceOn
5 Woods SC, Seeley RJ, Rushing PA, D'Alessio D, Tso P. A controlled high-fat diet induces an obese syndrome in rats. J Nutr. 2003 ; 133(4) : 1081-7.   DOI
6 Kim IS, Lee J, Lee JS, Shin DY, Kim MJ, Lee MK. Effect of fermented yacon (Smallanthus sonchifolius) leaves tea on blood glucose levels and glucose metabolism in high-fat diet and streptozotocin-induced type 2 diabetic mice. Korean J Nutr. 2010 ; 43(4) : 333-41.   DOI   ScienceOn
7 Choi J, Kang HJ, Kim SZ, Kwon TO, Jeong SI, Jang SI. Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells. Arch Pharm Res. 2013 ; 36(7) : 912-7.   DOI   ScienceOn
8 Cirico TL, Omaye ST. Additive or synergetic effects of phenolic compounds on human low density lipoprotein oxidation. Food Chem Toxicol. 2006 ; 44(4) : 510–6.   DOI   ScienceOn
9 Chirumbolo S, Marzotto M, Conforti A, Vella A, Ortolani R, Bellavite P. Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets. Clin Mol Allergy. 2010 ; 8 : 13.   DOI   ScienceOn
10 Shaik YB, Castellani ML, Perrella A, Conti F, Salini, V, Tete S, Madhappan B, Vecchiet J, De Lutiis MA, Caraffa A, Cerulli G. Role of quercetin (a natural herbalcompound) in allergy and inflammation. J Biol Regul Homeost Agents. 2006 ; 20(3-4) : 47-52.
11 Freidwald WT, Levy RI, Fredrickson DS. Estimation of concentration of the low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972 ; 18(6) : 499-502.
12 Kim SY, Lee WC, Kim HB, Kim SK. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats. J Korean Soc Food Sci Nutr. 1998 ; 27(6) : 1217-22.
13 Andallu B, Varadacharyulu NCh. Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Clin Chim Acta. 2003 ; 338(1-2) : 3-10.   DOI   ScienceOn
14 Kawakami Y, Hosokawa T, Morinaka T, Irino S, Hirano S. Antiatherogenic effect of guava leaf extracts inhibiting leucocyte-type 12-lipoxygenase activity. Food Chem. 2012 ; 131(4) : 1069–75.   DOI   ScienceOn
15 Kadowaki T, Yamauchi T, Kubota N, Hara K, Ueki K, Tobe K. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrom. J Clin Invest. 2006 ; 116(7) : 1784-92.   DOI   ScienceOn
16 Hundal RS, Krssak M, Dufour S, Laurent D, Lebon V, Chandramouli V, Inzucchi SE, Schumann WC, Petersen KF, Landau BR, Shulman GI. Mechanism by which metformin reduces glucose production in type 2 diabetes. Diabetes. 2000 ; 49(12) : 2063-9.   DOI   ScienceOn
17 Levin BE, Dunn-Meynell AA, Ricci MR, Cummings DE. Abnormalities of leptin and ghrelin regulation in obesity-prone juvenile rats. Am J Physiol Endocrinol Metab. 2003 ; 285(5) : E949-57.   DOI   ScienceOn
18 Myers MG, Cowley MA, Munzberg H. Mechanisms of leptin action and leptin resistance. Annu Rev Physiol. 2008 ; 70 : 537-56.   DOI   ScienceOn
19 Zhang S, Zhang Q, Zhang L, Li C, Jiang H. Expression of ghrelin and leptin during the development of type 2 diabetes mellitus in a rat model. Mol Med Rep. 2013 ; 7(1) : 223-8.   DOI
20 Sahu A, Nguyen L, O'Doherty RM. Nutritional regulation of hypothalamic leptin receptor gene expression is defective in diet-induced obesity. J Neuroendocrinol. 2002 ; 14(11) : 887-93.   DOI   ScienceOn
21 Gilbert ER, Fu Z, Liu D. Development of a nongenetic mouse model of type 2 diabetes. Exp Diabetes Res. 2011 ; 2011 : 416254.   DOI   ScienceOn
22 Park CY, Hong KE. A Study on the Effect of Herbal-acupuncture with HK-1000 solution on hyperlipidemia and arteriosclerosis induced rats. J Pharmacopuncture. 2008 ; 11(3) : 55-66.   DOI   ScienceOn
23 Tan MH, Johns D, Glazer NB. Pioglitazone Reduces Atherogenic Index of Plasmain Patients with Type 2 Diabetes. Clin Chem. 2004 ; 50(7) : 1184–8.   DOI   ScienceOn
24 Mahmoud MF, Hassan NA, El Bassossy HM, Fahmy A. Quercetin protects against diabetes-induced exaggerated vasoconstriction in rats: effect on low grade inflammation. PLoS One. 2013 ; 8(5) : e63784.   DOI
25 Kayo D, Takashi K, Masaoki H, Yosgiya H. Effect of mulberry leaves on lipid metabolism in rabbits fed a cholesterol diet. Japanese J Nutr Food. 1994 ; 47(1) : 15-22.   DOI
26 Friedewald WT, Levy RI, Fedreison DS. Estimation of concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem. 1972 ; 18(6) : 499-502.
27 Grover SA, Levinton C, Paquet S. Identifying Adults at Low Risk for Significant Hyperlipidemia: A Validated Clinical Index. J Clin Epidemiol. 1999 ; 52(1) : 49–55.   DOI   ScienceOn
28 Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957 ; 28(1) : 56-63.   DOI
29 Yang YR, Cho JY, Park YK. Isolation and Identification of Antioxidative Compounds 3,4-Dihydroxybenzoic acid from Black Onion. Korean J Food Preserv. 2012 ; 19(2) : 229-34.   DOI   ScienceOn
30 Kwon EH, Jung MA, Rhee SJ, Choi SW, Cho SH. Antioxidant Effect and Improvement of Lipid Metabolism pf Mullberry fruit, Mulberry Leaves and Silkworm Power with Different Mixing Ratios in Streptozotocin-Induced Diabetic Rats. Korean J Food Nutr. 2006 ; 39(2) : 91-9.
31 Byun SH, Park SM, Kim SC, Cho IJ. Anti-fibrotic Effect of Mori Folium Extract in Hepatic Stellate Cells. Kor J Herbology. 2013 ; 28(4) : 49-55.   DOI   ScienceOn
32 Kobayashi Y, Miyazawa M, Kamei A, Abe K, Kojima T. Ameliorative effects of mulberry (Morus alba L.) leaveson hyperlipidemia in rats fed a high-fat diet: induction offatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress. Biosci Biotechnol Biochem. 2010 ; 74(12) : 2385-95.   DOI   ScienceOn
33 Yoo SK, Kim MJ, Kim JW, Rhee SJ. Effects of YK-209 mulberry leaves on disaccharidase activites of small intestine and blood glucose-lowering in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr. 2002 ; 31(6) : 1071-7.   DOI   ScienceOn
34 Rondinone CM. Diabetes: the latest developments in inhibitors, insulin sensitizers. Expert Opin Ther Target. 2005 ; 9(2) : 415-8.   DOI   ScienceOn
35 El-Sayyad HI, El-Sherbiny MA, Sobh MA, Abou-El-Naga AM, Ibrahim MA Mousa SA. Protective effects of Morus alba leaves extract on ocular functions of pups from diabetic and hypercholesterolemic mother rats. Int J Biol Sci. 2011 ; 7(6) : 715-28.   DOI
36 Kim EJ, Kim GY, Kim YM, Choi KH, Jang SJ. Anti-obesity effect of mulberry leaves extraction in obese rats high-fat diet Korean J Orient Physiol Pathol. 2009 ; 23(4) : 831-6.
37 Shim JU, Lim KT. Glycoprotein Isolated from Morus indica Linne Enhances Detoxicant Enzyme Activities and Lowers Plasma Cholesterol in ICR Mice. Korean J Food Sci Techol. 2008 ; 40(6) : 691-5.
38 Lee SY, Park SL, Nam YD, Yi SH, Lim SI. Anti-diabetic Effects of Fermented Green Tea in KK-Ay Diabetic Mice. Korean J Food Sci Technol. 2013 ; 45(4) : 488-94.   DOI   ScienceOn
39 Al-Shoumer KA, Al-Asousi AA, Doi SA, Vasanthy BA. Serum leptin and its relationship with metabolic variables in Arabs with type 2 diabetes mellitus. Ann Saudi Med. 2008 ; 28(5) : 367-70.   DOI