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http://dx.doi.org/10.6116/kjh.2014.29.5.55.

The effects of Danggwisusan on restoration ability in wound induced animal models  

Bak, Ji Won (Department of Pathology, College of Oriental Medicine, Daejeon University)
Sim, Boo Yong (Department of Pathology, College of Oriental Medicine, Daejeon University)
Kim, Dong Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
Publication Information
The Korea Journal of Herbology / v.29, no.5, 2014 , pp. 55-63 More about this Journal
Abstract
Objectives : The purpose of this study was verification of the restoration ability effect of Danggwisusan extract(DG) in wound induced Rat. Methods : It needed to make a scar(around $2{\times}2cm^2$) on the top of the fascia in the back of the rats and then the rats were divided into 4groups(n=6). Control was not treated at all, where as DG was orally medicated DG, Terra was percutaneously applied Terramycin, and DG + Terra was both orally medicated DG and percutaneously applied Terramycin per day for three weeks. Results : 93% or higher cell viability was observed in all tested groups from 1, 10, $100{\mu}g/m{\ell}$ in RAW 264.7cells. The DG decreased NO and cytokine production activity dose dependently. The production of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ were decreased by 46%, 46% and 40% in DG treated $100{\mu}g/m{\ell}$. The size of wound was significantly decreasing in DG, Terra, DG + Terra. WBC was significantly reduced in DG and DG + Terra. Monocyte was significantly reduced in DG, Terra and DG + Terra. Neutrophil was also reduced in DG, DG + Terra but not meaningless. The mRNA expression of MMP-1 was significantly reduced in Terra, MMP-2 was significantly reduced in DG, Terra, DG + Terra, and MMP-9 was significantly reduced in DG + Terra. Conclusions : According to the results, we thought that DG showed anti-inflammatory activities on the RAW 264.7cells in mouse macrophage and in adult rat wound. Moreover, the progress of recovery was found visually, heamatologically, genetically and histopathologically. In conclusion, it could be thought that DG has effect on the treating of wound.
Keywords
Anti-inflammatory; Cyotokine; Danggwisusan; Matrix metalloprotease(MMP); Wound;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Choi WH, Oh YS, Ahn JY, Kim SR, Ha TY. Antioxidative and Protective Effects of Ulmus davidiana var. japonica Extracts on Glutamate-Induced Cytotoxicity in PC 12 Cells. Korean Soc Food Sci Technol. 2005 ; 37(3) : 479-83.   과학기술학회마을
2 Jung HM, Han HS, Lee YJ. Effect of Fermented Epimedii Herba Extract on the Cytotoxicity and Immuno modulating Activity. Kor J Herbology. 2013 ; 28(6) : 111-7.   DOI   ScienceOn
3 Lee YH, Kim WS. Morphological Observation on the Effects of Hyperbaric Oxygenation to the Cutaneous Wound Healing. Chungnam Med J. 1991 ; 18(1) : 89-97.
4 Chang JH, Hwang SH, Lee EJ, Nobuaki O, Lee JH. Effect of Panax ginseng C.A. Meyer Extract (Ginseol K-b1) on UVB-induced Skin Damage in Hairless Mice. East Asian Soc Dietary Life. 2012 ; 22(2) : 224-30.   과학기술학회마을
5 Liu ZY. wai ek pi fu ke de bian zheng lun zhi. Seo won dang. 1987 : 35
6 Lee YG. Effect of Astragali Radix on Dermoepidermal Recovery to Wound Tissues in Rats' Skin. Dongguk University. 2004.
7 Ryu IH, Cho HB, Kim SB, Seo YJ, Choi CM. The Inhibitory Effect of Picrasmae Lignum on Inflammatory Responses. J Orient Gynecol. 2011 ; 24(1) : 1-14.   과학기술학회마을
8 Clark RA. Biology of dermal wound repair. Dermatol Clin. 1993 ; 11(4) : 647-66.
9 Dang CM, Beanes S, Lee H, Zhang Xl, Soo C, Ting K. Scarless Fetal Wounds Are Associated with an Increased Matrix Metalloproteinase-to-Tissue-Derived Inhibitor of Metalloproteinase Ratio. Plast Reconstr Surg. 2003 ; 111(7) : 2273-85.   DOI   ScienceOn
10 Gross J, Lapiere CM. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci U S A. 1962 ; 48(6) : 1014-22.   DOI   ScienceOn
11 Soo C, Shaw W, Zhang XL, Longaker M. Howard E, Ting K. Differential expression of matrix metalloproteinases and their tissue-derived inhibitors in cutaneous wound repair. Plast Reconstr Surg. 2000 ; 105(2) : 638-47.   DOI   ScienceOn
12 Wysocki AB, Staiano-Coico L, Grinnell F. Wound Fluid from Chronic Leg Ulcers Contains Elevated Levels of Metalloproteinases MMP-2 and MMP-9. J Invest Dermatol. 1993 ; 101(1) : 64-8.   DOI   ScienceOn
13 Lewis JS, Lee JA, Underwood JC, Harris AL, Lewis CE. Macrophage responses to hypoxia: relevance to disease mechanisms. J Leukoc Biol 1999 ; 66(6) : 889-900.   DOI
14 Kim DH, Hwang EY, Son JH. Anti-Inflammatory Activity of Carthamus tinctoriousSeed Extracts in Raw 264.7 cells. J Life Sci. 2013 ; 23(1) : 55-62.   과학기술학회마을   DOI   ScienceOn
15 Lee DG. An experimental study on the wound healing effects of alginate in full rhickness excisional wound models of rat. Daegu Catholic University. 2004.
16 Mauch C, Krieg T, Bauer EA. Role of the extracellular matrix in the degradation of connective tissue. Arch Dermatol Res. 1994 ; 287(1) : 107-14.   DOI
17 Kurkinen M, Vaheri A, Roberts PJ, Stenman S. Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 1980 ; 43(1) : 47-51.
18 Woodley DT, O'Keefe EJ, Prunieras M. Cutaneous wound healing: a model for cell-matrix interactions. J Am Acad Dermatol 1985 ; 12(2 Pt 2) : 420-33.   DOI   ScienceOn
19 Kim TY, Jo NJ, Jeong HS, Kim MS. The Effects of Pulsed Ultrasound Treatment and Cold Therapy in the Process of Acute Wound Healing. J Korean Acad Clin Electrophysiol. 2007 ; 5(2) : 23-33.   과학기술학회마을
20 Han CD, Lee WM, Lee HB, Hahn MH, Yu WW. Effects of Steroid and Nonsteroidal Anti-inflammatory Drugs on Acute Wound Healing and Collagen Synthesis in Rat Skin. J Korean Orthop Assoc. 2003 ; 38(4) : 393-9   과학기술학회마을   DOI
21 Yoo YC, Yoo SG. The effects of compound madecassol on the wound healing. Arch Plast Surg. 1998 ; 25(8) : 1451-58.
22 Li C. Ui hak ib mun. Bub in moon hwa sa. 2009.
23 Kim YS. Study on the Effects of Angelicae sinensis Radix on Thrombosis. Kor J Herbology. 2000 ; 15(1) : 53-7.   과학기술학회마을
24 Heo Jun. Dong ui bo gam. Bub in moon hwa sa. 2012 : 1046-47, 1052.
25 Herbal medicine. Seoul : Younglimsa. 2007. 154-155, 236-38, 396-99, 462-64, 476-77, 583-85, 629-631.
26 Lee GH. Study on the Effects of Dangguixusan(當歸鬚散) on Experimental Blood Stasis Model Induced by Compression. Kyunghee Univ Oriental Med J. 1998 ; 22(1) : 22-38.
27 Joe WA, Jang MJ, Cheon SJ, Sung JY, Choi EY, Kang BY, Jung SY, Jeung YS, Kim YS, An BJ, Lee CE, Lee JT. Cosmeceutical activities and Anti-inflammatory effects of Shell from Persicae semen. Kor J Herbology. 2006 ; 21(2) 87-93.   과학기술학회마을
28 Textbook of plastic surgery. Chapter3. 2009 : 83-89.
29 Yang Jl. The Effect of Sildenafil on Wound Healing in Rat. Dong-A University. 2010.
30 Harding KG, Morris HL, Patel GK. Healing chronic wounds. BMJ. 2002 ; 324 : 160-3.   DOI   ScienceOn