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http://dx.doi.org/10.6116/kjh.2014.29.2.7.

Antidepressant-like effects of Nelumbo nucifera leaves extract in chronic mild stress model  

Kang, Min Gu (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Kim, Young Hwa (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Im, A Rang (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Nam, Byung Soo (Infrastructure Management Team, Korea Institute of Oriental Medicine)
Chae, Sung Wook (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Lee, Mi Young (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Publication Information
The Korea Journal of Herbology / v.29, no.2, 2014 , pp. 7-13 More about this Journal
Abstract
Objectives : Chronic mild stress (CMS) model is currently recognized as a better animal model of depression. The purpose of this study was to investigate the antidepressant-like effects of the Nelumbo nucifera leave extract using CMS model. Methods : The antidepressant-like effects of Nelumbo nucifera leaves extract was determined by using animal models of depression. Male ICR mice were divided into four groups: saline-treated normal, without CMS; saline-treated stress control; CMS+ Imipramine(20mg/kg); CMS+Nelumbo nucifera leaves extract(200mg/kg). All mice except the normal group exposed an unpredicted sequence of chronic mild stressors for 5 weeks. The behavior of mice were detected by sucrose preference test, forced swim test and tail suspension test. Then concentration of corticosterone in serum was detected by enzyme immunoassay. Results : Nelumbo nucifera leaves extract administration by daily gavage from the 3rd week exhibited an antidepressant-like effect on CMS-induced depression in mice. Nelumbo nucifera leaves extract administration at dose of 200 mg/kg significantly increased the sucrose consumption, and decreased the immobility durations in forced swim test and tail suspension test. Furthermore the corticosterone level decreased than control group. Conclusions : Chronic mild stress can affect mouse behavior and corticosterone level and cause depression. The present experiments not only further confirm the antidepressant-like effects of Nelumbo nucifera leaves extract in the sucrose preference test, forced swimming test and tail suspension test, but also the improving effects of Nelumbo nucifera leaves extract on the depression-like symptoms in the CMS model. Nelumbo nucifera leaves extract has the antagonism on CMS and produce antidepressive effects.
Keywords
Antidepressant; Nelumbo nucifera; Chronic mild stress; Forced swimming test; Tail suspension test; Sucrose preference test;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Gould E, McEwen BS, Tanapat P, Galea LA, Fuchs E. Neurogenesis in the dentate gyrus of the adult tree shrew is regulated by psychosocial stress and NMDA receptor activation. J Neurosci. 1997 ; 17(7) : 2492-8.
2 Kessler RC, McGonagle KA, Zhao S, Nelson CB, Hughes M, Eshleman S. Lifetime and 12-month prevalence of DSM-III-R psychiatric disorders in the United States. Results from the National Comorbidity Survey. Arch Gen Psychiatry. 1994 ; 51 : 8-19.   DOI   ScienceOn
3 Kim JH, Kim SY, Lee SY, Jang CG. Antidepressant-like effects of Albizzia julibrissin in mice: involvement of the 5-HT1A receptor system. Pharmacol Biochem Behav. 2007 ; 87 : 41-7.   DOI   ScienceOn
4 Czeh B, Michaelis T, Watanabe T, Frahm J, de Biurrun G, van Kampen M, Bartolomucci A, Fuchs E. Stress-induced changes in cerebral metabolites, hippocampal volume, and cell proliferation are prevented by antidepressant treatment with tianeptine. Proc Natl Acad Sci USA. 2001 ; 98(22) : 12796-801.   DOI   ScienceOn
5 Anderson, IM. SSRIs versus tricyclic antidepressants in depressed inpatients: a meta-analysis of efficacy and tolerability. Depress Anxiety. 1998 ; 7(1) : 11-7.   DOI
6 Garcia R. Stress, metaplasticity, and antidepressants. Curr Mol Med. 2002 ; 2(7) : 629-38.   DOI   ScienceOn
7 Tanapat P, Galea LA, Gould E. Stress inhibits the proliferation of granule cell precursors in the developing dentate gyrus. Int J Dev Neurosci. 1998 ; 16(3-4) : 235-9.   DOI   ScienceOn
8 Nestler EJ, Barrot M, DiLeone RJ, Eisch AJ, Gold SJ, Monteggia LM. Neurobiology of Depression. Neuron. 2002 ; 34(1) : 13-25.   DOI   ScienceOn
9 Anderson, I.M. Meta-analytical studies on new antidepressants. Br Med Bull. 2001 ; 57 : 161-78.   DOI   ScienceOn
10 Bouvier N, Trenque T, Millart H. Development of antidepressant drugs. Experience and prospects. Presse Med. 2003 ; 32(11) : 519-22.
11 Mandrioli R, Mercolini L, Saracino MA, Raggi MA. Selective serotonin reuptake inhibitors (SSRIs): therapeutic drug monitoring and pharmacological interactions. Curr Med Chem. 2012 ; 19(12) : 1846-63.   DOI
12 Steffens DC, Krishnan KR, Helms MJ. Are SSRIs better than TCAs? Comparison of SSRIs and TCAs: a meta-analysis. Depress Anxiety. 1997 ; 6(1) : 10-8.   DOI
13 Katz RJ, Roth KA, Carroll BJ. Acute and chronic stress effects on open field activity in the rat: implications for a model of depression. Neurosci Biobehav Rev. 1981 ; 5(2) : 247-51.   DOI   ScienceOn
14 Sarko J. Antidepressants, old and new. A review of their adverse effects and toxicity in overdose. Emerg Med Clin North Am. 2000 ; 18(4) : 637-54.   DOI
15 Willner P. The validity of animal models of depression. Psychopharmacology. 1984 ; 83(1) : 1-16.   DOI
16 Shin KH. Animal models of depression and screening of antidepressants. Kor J Psychopharmacol. 2000 ; 11(4) : 291-303.
17 Willner P, Towell A, Sampson D, Sophokleous S, Muscat R. Reduction of sucrose preference by chronic unpredictable mild stress, and its restoration by a tricyclic antidepressant. Psychopharmacology (Berl). 1987 ; 93(3) : 358-64.
18 Luo DD, An SC, Zhang X. Involvement of hippocampal serotonin and neuropeptide Y in depression induced by chronic unpredicted mild stress. Brain Res Bull. 2008 ; 77(1) : 8-12.   DOI   ScienceOn
19 Gracia R, Vouimba RM, Jaffard R. Contextual conditioned fear blocks the induction but not the maintenance of lateral septal LTP in behaving mice. J Neurophysiol. 1997 ; 78(1) : 76-81.   DOI
20 Pavlides C, Nivon LG, McEwen BS. Effect of chronic stress on hippocampal long-term potentiation. Hippocampus. 2002 ; 12(2) : 245-57.   DOI   ScienceOn
21 Kessal K, Deschaux O, Chessel A, Xu L, Moreau JL, Garcai R. Fluoxeti reverses stress-induced fimbria-prefrontal long-term potentiation facilitation. Neuroreport. 2006 ; 17(3) : 319-22.   DOI   ScienceOn
22 Ahn DG. Illustrated Book of Korean Medical Herbs. 7th ed. Seoul : Kyohaksa. 1998 : 684.
23 Liu S, Li D, Huang B, Chen Y, Lu X, Wang Y. Inhibition of pancreatic lipase, ${\alpha}$-glucosidase, ${\alpha}$-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves. J Ethnopharmacol. 2013 ; 149(1) : 263-9.   DOI   ScienceOn
24 Yang WM, Kim HM, Chang MS, Kim WN, Kim SW, Choi DG, Lee HC, Kim YK, Park SK. Effects of ethanol extract of Nelumbo nucifera leaves on Anti-oxidation and Type I procollagen expression in CCD-986sk cell. Kor J Orient Med prescription. 2006 ; 14(2) : 67-75.
25 Kim SB, Rho SB, Rhyu DY, Kim DW. Effect of Nelumbo nucifera leaves on hyperlipidemic and atherosclerotic bio F1B hamster. Kor J Pharmacogn. 2005 ; 36(3) : 229-34.   과학기술학회마을
26 Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacology (Berl). 1985 ; 85(3) : 367-70.   DOI   ScienceOn
27 Ono Y, Hattori E, Fukaya Y, Imai S, Ohizumi Y. Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J Ethnophamacol. 2006 ; 106(2) : 238-44.   DOI   ScienceOn
28 Porsolt RD, Le Pichon M, Jalfre M. Depression: a new animal model sensitive to antidepressant treatments. Nature. 1977 ; 266 (5604) : 730-2.   DOI   ScienceOn
29 Park SK, Kim TI, Lee WK, Park HK, Hong TJ. Combination of green tea extract and L-theanine alleviates electric foot shock induced stress by modulating neurotransmitters in mice. Yakhak Hoeji. 2009 ; 53(5) : 241-9.   과학기술학회마을
30 Kim JJ, Lee HJ, Han JS, Packard MG. Amygdala is critical for stress-induced modulation of hippocampal long-term potentiation and learning. J Neurosci. 2001 ; 21(14) : 5222-8.
31 Konkle AT, Baker SL, Kentner AC, Barbagallo LS, Merali Z, Bielajew C. Evaluation of the effects of chronic mild stressors on hedonic and physiological responses: sex and strain compared. Brain Res. 2003 ; 992(2) : 227-38.   DOI   ScienceOn
32 Yang CH, Huang CC, Hsu KS. Behavioral stress modifies hippocampal synaptic plasticity through corticosterone-induced sustained extracellular signal-regulated kinase/mitogen- activated protein kinase activation. J Neurosci. 2004 ; 24(49) : 11029-34.   DOI   ScienceOn
33 Watanabe Y, Gould E, McEwen BS. Stress induces atrophy of apical dendrites of hippocampal CA3 pyramidal neurons. Brain Res. 1992 ; 588(2) : 341-5.   DOI   ScienceOn
34 Kim JJ, Foy MR & Thompson RF. Behavioral stress modifies hippocampal plasticity through NMDA receptor activation. Proc Natl Acad Sci USA. 1996 ; 93(10) : 4750-3.   DOI
35 An L, Zhang YZ, Yu NJ, Liu XM, Zhao N, Yuan L, Li YF. Role for serotonin in the antidepressant-like effects of a flavonoid extract of Xiaobuxin-Tang. Pharmacol Biochem Behav. 2008 ; 89(4) : 572-80.   DOI