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http://dx.doi.org/10.6116/kjh.2014.29.2.33.

Anti-inflammatory Effects of Cnidium Rhizoma against Intracerebral Hemorrhage in Rats  

Baek, Dong-Ha (Department of Anatomy-Pointology, College of Oriental Medicine, Gachon University)
Kim, Do-Hoon (Department of Oriental Medical Classics & History, Gachon University)
Kim, Youn-Sub (Department of Anatomy-Pointology, College of Oriental Medicine, Gachon University)
Publication Information
The Korea Journal of Herbology / v.29, no.2, 2014 , pp. 33-38 More about this Journal
Abstract
Objectives : Inflammation is mediated by cellular components, such as leukocytes and microglia, and molecular components, including cytokines, extracellular proteases, and reactive oxygen species. Cnidium Rhizoma effects the anti-inflammatory, antioxidant, suppression of the microglia activation and protection of the nerve cell injury. For this reason, we investigated the anti-inflammatory effects of water extracts of Cnidium Rhizoma on intracerebral hemorrhage (ICH). Method : ICH was induced by the stereotaxic intracerebral injection of bacterial collagenase type IV (0.23 $U/{\mu}{\ell}$, 0.1 ${\mu}{\ell}/min$) in Sprague-Dawley rats. We orally administrated once 3 hours after ICH, then 2 times at 24-hour intervals the water extracts of Cnidium Rhizoma (500 mg/kg), myeloperoxidase (MPO) was observed by using immunofluorescense and expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and microglia were observed by using immunohistochemistry. Results : Infiltration of MPO expressing neutrophil, expression of iNOS and TNF-${\alpha}$ and activated microglia were significantly reduced in peri-hematoma of the rats fed with water extracts of Cnidium Rhizoma. Conclusion : These results demonstrated that water extracts of Cnidium Rhizoma suppressed an inflammatory reaction through inhibition of MPO, iNOS and TNF-${\alpha}$ positive cell and activated microglia number in peri-hematoma of ICH-induced rats.
Keywords
Cnidium Rhizoma (CR); MPO; iNOS; TNF-${\alpha}$; activated microglia; ICH;
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