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http://dx.doi.org/10.6116/kjh.2013.28.6.119.

Effects of β-Asarone on Pro-Inflammatory Cytokines and Learning and Memory Impairment in Lipopolysaccharide-Treated Mice  

Choi, Moon-Sook (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Kwak, Hee-Jun (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Kweon, Ki-Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Hwang, Ji-Mo (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Shin, Jung-Won (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Sohn, Nak-Won (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.28, no.6, 2013 , pp. 119-127 More about this Journal
Abstract
Objectives : ${\beta}$-Asarone (BAS) is an active ingredient in Acori Rhizoma. This study investigated anti-neuroinflammatory and memory ameliorating effects of BAS in systemic lipopolysaccharide (LPS)-treated C57BL/6 mice. Methods : BAS was administered orally at doses of 7.5, 15, and 30 mg/kg for 3 days prior to LPS (3 mg/kg, intraperitoneal) injection. Pro-inflammatory cytokine mRNA, including tumor necrosis factor-${\alpha}$ (TNF-ㅍ), interleukin (IL)-$1{\beta}$ and IL-6, was measured in hippocampus tissue using real-time polymerase chain reaction at 4 h after the LPS injection. An ameliorating effect of 30 mg/kg BAS on learning and memory impairment in the LPS-treated mice was verified using the Morris water maze test. Results : BAS significantly attenuated up-regulation of TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 mRNA in hippocampus tissue of the LPS-treated mice. In acquisition training test, BAS improved learning performance of the LPS-treated mice with a significant decrease of escape latency to the platform. In memory retention test, BAS also ameliorated memory impairment of the LPS-treated mice with a significant increase of swimming time in zones neighboring to the platform, number of target heading, and memory score. Conclusion : The results suggest that inhibition of pro-inflammatory cytokines and neuroinflammation in the hippocampus by BAS could be one of the mechanisms for BAS-mediated ameliorating effect on learning and memory impairment in LPS-treated mice.
Keywords
${\beta}$-Asarone; Acori Rhizoma; neuroinflammation; memory impairment; Morris water maze;
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