Browse > Article
http://dx.doi.org/10.6116/kjh.2013.28.4.71.

Comparative Study on Anti-inflammatory Effects and Compound Contents of Multi-herbal Extracts HT008 produced by Different Manufacturing Methods  

Lee, Donghun (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
Song, Jungbin (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
Song, Mikyung (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
Gaire, Bhakta Prasad (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
Park, Juyeon (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.)
Jin, Aihong (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.)
Choi, Ho-Young (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
Kim, Hocheol (Dept. of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.28, no.4, 2013 , pp. 71-76 More about this Journal
Abstract
Objectives : The biological activities and compound contents of herbal medicine vary depending on manufacturing processes. In this study, we compared anti-inflammatory effects and compound contents of three kinds of multi-herbal extract HT008 produced by different manufacturing processes in order to determine chemical and biological equivalence. Methods : HT008 was produced by three different manufacturing methods: 1. Freeze dried extract of Eleutherococcus senticosus, Scutellaria baicalensis and Angelica sinensis (HT008 FD), 2. Spray dried extract of E. senticosus and S. baicalensis combined with reflux extract of A. sinensis (HT008 SD), 3. Spray dried extract of E. senticosus and S. baicalensis combined with supercritial fluid extract of A. sinensis (HT008 SF). Anti-inflammatory effects were evaluated using acetic acid induced pain model and ${\lambda}$-carageenan induced paw edema model. Compound contents were evaluated by HPLC quantitative analysis of standard compounds of HT008, eleutheroside E, baicalin, z-ligustilide. Results : HT008 FD, HT008 SD and HT008 SF significantly decreased acetic acid induced pain index and ${\lambda}$-carrageenan induced paw edema volume compared with that of control group. There was no significant difference in efficacy among the HT008 FD, HT008 SD and HT008 SF. Standard compound contents of HT008 FD, HT008 SD and HT008 SF were quantified within the range of Korean pharmacopoeia or other research. Conclusions : Three different manufacturing methods of multi-herbal extracts have been developed without noticeable difference in the efficacy or compound contents. The results might be used to establish manufacturing process and industrialization of herbal extracts.
Keywords
comparison of efficacy; compound contents; manufacturing methods; HT008; acetic acid; ${\lambda}$-carageenan; supercritical fluid extraction;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Yim HJ, Jung IC. The effects of KagamSinKiHwan (KSKH) hot water extract & ultra-fine powder on proinflammatory cytokine of microglia & memory deficit of amnesia mice model. J Ori Neuropsychiatr. 2008 ; 19(3) : 85-100.   과학기술학회마을
2 Arun S M. Handbook of Industrial Drying. CRC Press. Boca Raton. 2006 : 20-1.
3 Franks F. Freeze-drying of bioproducts: putting principles into practice. Eur J Pharm Biopharm. 1998 ; 45(3) : 221-9.   DOI   ScienceOn
4 Mujumdar SA, Huang LX, Chen XD. An overview of the recent advances in spray-drying. Dairy Sci Technol. 2010 ; 90 : 211–24.   DOI
5 Lucchesi ME, Chemat F, Smadja J. Solvent-free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro-distillation. J Chromatogr A. 2004 ; 1043(2) : 323-7.   DOI   ScienceOn
6 Baritaux O, Richard H, Touche J, Derbesy M. Effects of drying and storage of herbs and spices on the essential oil. Flav Fragr J. 1992 ; 7(5) : 267–71.   DOI
7 Kim NM, Kim DH, Quality change of cinnamon extract prepared with various drying methods. Kor J Food Nutr. 2000 ; 13(2) : 152-7.   과학기술학회마을
8 Choi SR, Jang I, Kim CS, You DH, Kim JY, Kim YG. Changes of components and quality in gastrodiae rhizoma by different dry methods. Kor J Med Crop Sci. 2011 ; 19(5) : 354-61.   과학기술학회마을   DOI   ScienceOn
9 Kim MJ, Chu WM, Park EJ. Antioxidant and antigenotoxic effects of Shiitake mushrooms affected by different drying methods. J Korean Soc Food Sci Nutr. 2012 ; 41(8) : 1041-8.   과학기술학회마을   DOI   ScienceOn
10 Li P, Li SP, Lao SC, Fu CM, Kan KK, Wang YT.. Optimization of pressurized liquid extraction for Z-ligustilide, Z-butylidenephthalide and ferulic acid in Angelica sinensis. J Pharm Biomed Anal. 2006 ; 40(5) : 1073-9.   DOI   ScienceOn
11 Xie JJ, Lu J, Qian ZM, Yu Y, Duan JA, Li SP. Optimization and comparison of five methods for extraction of coniferyl ferulate from Angelica sinensis. Molecules. 2009 ; 14(1) : 555-65.   DOI   ScienceOn
12 Jung SM, Schumacher HR, Kim H, Kim M, Lee SH, Pessler F. Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2 levels. Arthritis Res Ther. 2007 ; 9(4) : R64.   DOI   ScienceOn
13 Lim DW, Kim MY, Song M, Kim J, Lee D, Park J, Bu Y, Kim H. Effect of New Herbal Mixture, HT008 on Arthritis Rat Model. Kor J herbology. 2009 ; 24(4) : 197-204.   과학기술학회마을
14 Korea Food & Drug Administration. Korean Pharmacopoeia. 9th edition. 2007.
15 Song MK, Kim MY, Kim H. Standardization of Eleutherococcus species and HPLC Method Validation for Quantitative Analysis. Kor J Herbology. 2011 ; 26(1) : 103-10.   과학기술학회마을
16 Kim KW, Jeong HJ, Chung GY, Sohn HJ, Oh SM, Kim SY, Nam SH, Park JH, Shim YE. Comparison on the Phthalides content of Cinidil Rhizoma and Angelicae Radix cultivated in Korea and China. Korean J Plant Res. 2001 ; 14(1) : 24-31.   과학기술학회마을
17 Yamazaki T, Matsumura T, Tsukiyama T, Tokiwa T. Anti-inflammatory effect of eleutheroside E from Acanthopanax senticosus. Tiss Cult Res Comm. 2006 ; 25(3) : 137-45.
18 Chung JW, Choi RJ, Seo EK, Nam JW, Dong MS, Shin EM, Guo LY, Kim YS. Anti-inflammatory effects of (Z)-ligustilide through suppression of mitogen-activated protein kinases and nuclear factor-$\kappa{B}$ activation pathways. Arch Pharm Res. 2012 ; 35(4) : 723-32.   과학기술학회마을   DOI   ScienceOn
19 Lu JL, Zhao J, Duan JA, Yan H, Tang YP, Zhang LB. Quality Evaluation of Angelica sinensis by Simultaneous Determination of Ten Compounds Using LC-PDA. Chromatographia. 2009 ; 70(3-4) : 455-65.   DOI
20 Cherng CH, Lee KC, Chien CC, Chou KY, Cheng YC, Hsin ST, Lee SO, Shen CH, Tsai RY, Wong CS. Baicalin ameliorates neuropathic pain by suppressing HDAC1 expression in the spinal cord of spinal nerve ligation rats. J Formos Med Assoc. 2013 ; pii : S0929-6646 (13) 00134-4.
21 Je HD. Antinociceptive and anti-inflammatory properties of Cinnamomum cassia derived-cinnamaldehyde in rodents. Yakhak Hoeji. 2012 ; 56(4) : 236-9.   과학기술학회마을
22 Franzotti EM, Santos CV, Rodrigues HM, Mourao RH, Andrade MR, Antoniolli AR. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva-branca). J Ethnopharmacol. 2000 ; 72(1-2) : 273-7.   DOI   ScienceOn
23 Saito H, Nomura Y. Screening methods for drug evaluation 3. Pharmaceutical Research and Development. Tokyo, Hirokawa. 1989 : 22.
24 Lee YS, Park JY, Lim HJ. Peripheral analgesic effects of NSAIDs and expressions of cyclooxygenase-1 and cyclooxygenase-2 in Acute arthritic model in rats. Korean J Anesthesiol. 2004 ; 47 : 862-9.   과학기술학회마을   DOI
25 Costa B, Colleoni M, Conti S, Parolaro D, Franke C, Trovato AE, Giagnoni G. Oral anti-inflammatory activity of cannabidiol, a non-psychoactive constituent of cannabis, in acute carrageenan-induced inflammation in the rat paw. Naunyn Schmiedebergs Arch Pharmacol. 2004 ; 369(3) : 294-9.   DOI   ScienceOn
26 Park EH, Hwang SE, Kahng JH. Anti-inflammatory activity of Opuntia ficus-indica. Yakhak Hoeji. 1998 ; 42(6) : 621-6.   과학기술학회마을