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http://dx.doi.org/10.6116/kjh.2013.28.2.9.

Effects of the ethanol extract of Codonopsis Pilosulae Radix on ovalbumin-induced allergic responses in mice  

Kang, Seok Yong (Department of Herbology, College of Oriental Medicine, Dongguk University)
Jung, Jin Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
Lee, Sang Kook (College of Pharmacy, Natural Products Research Institute, Seoul National University)
Lee, Seung Ho (College of Pharmacy, Yeungnam University)
Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
Publication Information
The Korea Journal of Herbology / v.28, no.2, 2013 , pp. 9-15 More about this Journal
Abstract
Objectives : The root of Codonopsis pilosula (Fr.) Nannf. (Codonopsis Pilosulae Radix) has been traditionally used as a oriental medicine with an anti-thrombotic, antidiabetic, anticancer, and anti-gastric ulcer effects and immunological adjuvant. In this study, we investigated the effect of 70% ethanol extract of Codonopsis Pilosulae Radix (CPR-E) on ovalbumin (OVA)-induced allergic responses in mice. Methods : Mice were sensitized (1, 8, and 15 days) with OVA and airway challenged(22, 24, 26, 28, and 30 days) to induced allergic responses. CPR-E extract at doses of 50 and 100 mg/kg/body weight was orally administered from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specific immunoglobulin (Ig) E, and Th1/Th2 cytokines such as IFN-${\gamma}$ and IL-4 were measured in the sera of mice by ELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining. Results : CPR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and IL-4 compared with those of OVA control group, but significantly increased the serum level of IFN-${\gamma}$. Based on H&E staining, CPR-E extract inhibited the infiltration of inflammatory cells into lung tissues with histological changes. Conclusions : These results indicate that CPR-E extract has anti-inflammatory and anti-allergic responses through regulating the cytokine balance, suggesting that the extract may be useful for the treatment of allergic inflammatory diseases such as bronchial asthma and allergic rhinitis.
Keywords
Codonopsis Pilosulae Radix; ovalbumin; allergic response; inflammation; Th1/Th2 cytokine;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Jung JK, Park YK. Dose range finding study of KOB03, a new polyherbal medicine for the treatment of allergic rhinitis, by oral administration for four weeks in Sprague-Dawley rats. Kor J Herbology. 2012 ; 27(3) : 101-6.   과학기술학회마을   DOI   ScienceOn
2 Park YC, Lim JD, Park YK, Yoon MS, Lee SD. Review : Clinical application and efficacy of herbal medicines by modulating cytokines in atopic dermatitis-induced animal model. Kor J Herbology. 2012 ; 27(4) : 33-44.   과학기술학회마을   DOI   ScienceOn
3 Korean Journal of Asthma, Allergy and Clinical Immunology. Asthma and Allergy disease. Seoul : Koonja publisher. 2002 ; 66 : 431-3.
4 seoul National University College of Medicine. Immunology. Seoul : Seoul National University publisher. 1987 : 188-97.
5 Ahn KM. Role of mast cell in allergic inflammation and innate immunity. Korean J Pediatrics. 2004 ; 47(11) : 1137-41.
6 Kim SH, Kim SA, Park MK, Kim SH, Park YD, Na HJ, Kim HM, Shin MK, Ahn KS. Paeonol inhibits anaphylactic reaction by regulation histamine and TNF-${\alpha}$. International Immunopharmacology. 2004 ; 4 : 279-87.   DOI   ScienceOn
7 Kawakami T, Galli SJ. Regulation of mast cell and basophil function and survival by IgE. Nature Reviews Immunology. 2002 ; 2 : 773-86.   DOI   ScienceOn
8 Choi KJ. Effect of Saururus chinensis (Lour.) Baill Aquacupuncture on Allergy in Mice. Dongeui university. 2004 : 1-23.
9 Hong WS. JungkyoHuangjenaegyeongsomun. seoul : Dongyang medicine Institute publisher. 1981 : 24, 39, 119.
10 The whole country a college of Orental medicine The joint textbook publish commission compilatioin. herbology. seoul : yonglimsa. 2006. 578-9.
11 Ryu HY, Ahn SM, Kim JS, Sohn HY. Evaluation of In-vitro Anticoagulation Activity of 33 Different Medicinal Herbs. Journal of Life Science. 2010 ; 20(6) : 922-8.   과학기술학회마을   DOI   ScienceOn
12 Chen HT, Tsai YL, Chen YS, Jong GP, Chen WK, Wang HL, Tsai FJ, Tsai CH, Lai TY, Tzang BS, Huang CY, Lu CY. Dangshen (Codonopsis pilosula) activates IGF-I and FGF-2 pathways to induce proliferation and migration effects in RSC96 Schwann cells. Am J Chin Med. 2010 ; 38(2) : 359-72.   DOI   ScienceOn
13 He K, Li X, Chen X, Ye X, Huang J, Jin Y, Li P, Deng Y, Jin Q, Shi Q, Shu H. Evaluation of antidiabetic potential of selected traditional Chinese medicines in STZ-induced diabetic mice. J Ethnopharmacol. 2011 ; 137(3) : 1135-42.   DOI   ScienceOn
14 Sun YX. Immunological adjuvant effect of a water-soluble polysaccharide, CPP, from the roots of Codonopsis pilosula on the immune responses to ovalbumin in mice. Chem Biodivers. 2009 ; 6(6) : 890-6.   DOI   ScienceOn
15 Wang ZT, Du Q, Xu GJ, Wang RJ, Fu DZ, Ng TB. Investigations on the protective action of Condonopsis pilosula (Dangshen) extract on experimentally-induced gastric ulcer in rats. Gen Pharmacol. 1997 ; 28(3) : 469-73.   DOI   ScienceOn
16 Xu C, Liu Y, Yuan G, Guan M. The contribution of side chains to antitumor activity of a polysaccharide from Codonopsis pilosula. Int J Biol Macromol. 2012 ; 50(4) : 891-4.   DOI   ScienceOn
17 Reiss Y, Proudfoot AE, Power CA, Campbell JJ, Butcher EC. CC chemokine receptor (CCR)4 and the CCR10 ligand cutaneou T cell-attracting chemokine (CTACK) in lymphocyte trafficking to inflamed skin. J Exp Med. 2001 ; 194(10) : 1541-7.   DOI   ScienceOn
18 Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Biochem Biophys Res Commun. 2009 ; 387(3) : 435-9.   DOI   ScienceOn
19 Oh SW, Pae CI, Lee DK, Jones F, Chiang GK, Kim HO, Moon SH, Cao B, Ogbu C, Jeong KW, Kozu G, Nakanishi H, Kahn M, Chi EY, Henderson WR Jr. Tryptase inhibition blocks airway inflammation in a mouse asthma model. J Immunol. 2002 ; 168(4) : 1992-2000.   DOI
20 Sun YX. Immunological adjuvant effect of a water-soluble polysaccharide, CPP, from the roots of Codonopsis pilosula on the immune responses to ovalbumin in mice. Chem Biodivers. 2009 ; 6(6) : 890-6.   DOI   ScienceOn
21 Kim HJ, Hwang SY, Mok JY, Hwang BS, Jeong SI, Jang SI. Gagam-Gongjin-dan Extract Attenuates Immune Responses to Ovalbumin in Balb/c Mice. Kor J Herbology. 2009；24(4)：127-35.   과학기술학회마을
22 Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matsuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Biochem Biophys Res Commun. 2009 ; 387(3) : 435-9.   DOI   ScienceOn
23 Kimura M, Sasada T, Kanai M, Kawai Y, Yoshida Y, Hayashi E, Iwata S, Takabayashi A. Preventive effect of a traditional herbal medicine, Hochuekki-to, on immunosuppression induced by surgical stress. Surg Today. 2008 ; 38(4) : 316-22.   DOI   ScienceOn
24 Kralovec JA, Power MR, Liu F, Maydanski E, Ewart HS, Watson LV, Barrow CJ, Lin TJ. An aqueous Chlorella extract inhibits IL-5 production by mast cells in vitro and reduces ovalbumininduced eosinophil infiltration in the airway in mice in vivo. Int Immunopharmacol. 2005 ; 5(4) : 689-98.   DOI   ScienceOn
25 Weber A, Knop J, Maurer M. Pattern analysis of human cutaneous mast cell populations by total body surface mapping. Br J Dermatol. 2003 ; 148(2) : 224-8.   DOI   ScienceOn
26 Schwartz LB, Huff TF. Biology of mast cells. In : Middleton E, editor. Allergy : Principles and practice. 5th ed. Mosby St. Louis. 1998 : 261-76.
27 Koh YY. Pathophysiology of asthma. Pediatr Allergy Respir Dis. 2000 ; 10(4) : 255-62.
28 Kim YH. The role of mast cells in innate and adaptive immunity. J Life Sci. 2008 ; 18(6) : 891-6.   과학기술학회마을   DOI   ScienceOn
29 Jung HY. Molecular bioloy of cytokines. worldscience publishing company. 2002 : 117-24.